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Plasmid#171208Purposebacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV proteaseDepositorInsertpgl-3 (pgl-3 Nematode)
UseTagsMBP::HIS::TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDSP33
Plasmid#199375PurposeEncodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEZ-1
Plasmid#51868PurposeExpression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegansDepositorInsertpsdhb-1::mtLS-cpYFP
UseTagsExpressionWormMutationPromotersdhb-1Available sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab-3P::his-24::tagBFP
Plasmid#124343PurposeNuclear Localized Pan-Neuronal tagBFPDepositorInsertrab-3, tagBFP (rab-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNM3725
Plasmid#154113PurposeUAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vectorDepositorInsertUAS 11X::∆pes-10::GFP-C1::tbb-2 3'
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEY48
Plasmid#191052Purposeklp-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertklp-6 (klp-6 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY47
Plasmid#191051Purposegcy-21 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertgcy-21 (gcy-21 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDSP32
Plasmid#199374PurposeEncodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD400
Plasmid#137024PurposeSapTrap mNG-GLO donorDepositorInsertC. elegans GLO-optimized mNeonGreen
UseCRISPR; Saptrap donor for cloning repair templat…TagsC. elegans GLO-optimized mNeonGreenExpressionWormMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNM3813
Plasmid#154121Purposemec-4 promoter driving lexA-L-QFAD in an RCME vectorDepositorInsertmec-4p::lexA-L-QFAD::act-4 '3
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNM3811
Plasmid#154120Purposemec-4 promoter driving QF2 in an RCME vectorDepositorInsertmec-4p::QF2::act-4 '3
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNM3727
Plasmid#154115PurposelexO 5X ∆pes-10 promoter driving GFP-C1 in an RCME vectorDepositorInsertlexO 5X::∆pes-10::GFP-C1::tbb-2 3'
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNM3726
Plasmid#154114PurposeQUAS 5X ∆pes-10 promoter driving GFP-C1 in an RCME vectorDepositorInsertQUAS 5X::∆pes-10::GFP-C1::tbb-2 3'
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
Plasmid#159798PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmTagBFP2
Plasmid#184934PurposeCodon optimized blue fluorescent protein mTagBFP2 in C.elegans expresison plasmidDepositorInsertmTagBFP2
UseTagsExpressionWormMutationPromotertag-168Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-17b_Unc104-KIF5B-HALO
Plasmid#169996PurposeUltra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labelingDepositorInsertKinesin-like protein Unc-104 fused with KIF5B
UseTagsHALO-tag, 6xHISExpressionBacterialMutationPromoterT7Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
Plasmid#159803PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHJL3
Plasmid#199378PurposeEncodes dual cistronic cytosolic jGCaMP7s and mScarlet-I optimized for C. elegans expressionDepositorInsertjGCaMP7s::SL2::mScarlet-I
UseTagsExpressionBacterialMutationno mutationsPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMLS260
Plasmid#73685PurposePunc-17::2xNLS-FLP-D5 Expression vectorDepositorInsertPunc-17::2xNLS-FLP-D5::let-858 3'-UTR
UseFlp/frtTagsExpressionWormMutationG5DPromoterPunc-17Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only