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Showing: 1 - 20 of 35 results

  1. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Gavin Thomas, Emmanuele Severi
    Insert
    F5-flanked tetracycline resistance cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Promoter
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  2. pES134

    Plasmid
    #127551
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Gavin Thomas, Emmanuele Severi
    Article
    Wu et al Microbiology (Reading). 2024 Mar;170(3).
    Insert
    F3-flanked spectinomycin resistance cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127551-plasmid-map-sequence-id-251734

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  6. TFORF3101

    Plasmid
    #144577
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PES1 (PES1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144577-plasmid-map-sequence-id-404272

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  7. TFORF0162

    Plasmid
    #142471
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PES1 (PES1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142471-plasmid-map-sequence-id-397255

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  8. TFORF0163

    Plasmid
    #142472
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PES1 (PES1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142472-plasmid-map-sequence-id-397209

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  9. L4438

    Plasmid
    #1653
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    RNAi
    Tags
    pes10UAS-270, pes10Tsa, IVSA, SV40NLS, IVSL
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1653-plasmid-map-sequence-id-263

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  10. L4437

    Plasmid
    #1652
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    RNAi
    Tags
    pes10UAS-270, pes10Tsa, IVSA, SV40NLS, T7polN, T7…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1652-plasmid-map-sequence-id-262

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  11. pPD177.62

    Plasmid
    #1701
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    pes10UAS-270, pes10Tsa, IVSA, SV40NLS, TetRN, Tet…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1701-plasmid-map-sequence-id-307

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  12. pPD177.66

    Plasmid
    #1717
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, pes10UAS-270, pes10Tsa, T7p, gfpN, IVSR, gf…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1717-plasmid-map-sequence-id-323

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  13. pPD177.53

    Plasmid
    #1702
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    pes10UAS-270, pes10Tsa, IVSA, SV40NLS, TetRN, Tet…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1702-plasmid-map-sequence-id-308

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  14. pPD176.86

    Plasmid
    #1716
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, pes10UAS-270, pes10Tsa, T7p, gfpN, IVSR, gf…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1716-plasmid-map-sequence-id-322

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  15. pPD177.71

    Plasmid
    #1718
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, pes10UAS-270, pes10Tsa, T7p, gfpN, IVSR, gf…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1718-plasmid-map-sequence-id-324

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  16. L4753

    Plasmid
    #1676
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, pes10UAS-270, pes10Tsa, T7p, gfpN, IVSR, gf…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1676-plasmid-map-sequence-id-286

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  17. L4752

    Plasmid
    #1675
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, pes10UAS-270, pes10Tsa, T7p, gfpN, IVSR, gf…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1675-plasmid-map-sequence-id-285

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  18. pPD95_25

    Plasmid
    #1486
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, ATG-SecSig, pes10
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1486-plasmid-map-sequence-id-97

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  19. L4053

    Plasmid
    #1631
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, ATG-SecSig, pes10
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1631-plasmid-map-sequence-id-241

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  20. L4051

    Plasmid
    #1629
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, ATG-SecSig, pes10
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1629-plasmid-map-sequence-id-239

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Showing: 1 - 20 of 35 results