Showing: 121 - 140 of 153 results
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Plasmid#127670PurposeAmpicillin resistant multiplexing backbone without CRY2HA and mCherryDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
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S12.2_Bridge + Target
Plasmid#127671PurposeAmpicillin resistant multiplexing backbone with CRY2HA and mCherryDepositorTypeEmpty backboneUseCRISPRTagsHAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHAGE-UBC-TagRFP-T-ps
Plasmid#178656PurposeExpression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cellsDepositorInsertTagRFP-T with palmitoylation signal at N-terminus
UseLentiviralTagsPalmitoylation signalExpressionMutationPromoterUbcAvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC1-1-170
Plasmid#173467PurposePGRMC1 lacking the extreme C-terminusDepositorInsertPGRMC1 (PGRMC1 Human)
UseTagsFLAGExpressionMammalianMutationDeleted for amino acids 171-194PromoterAvailabilityAcademic Institutions and Nonprofits only -
PB533-42B3mutAC2-sfGFP
Plasmid#186777PurposeRNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cellsDepositorInsert42B3mutAC2-scFv
UseTagssfGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
myc-hRAD18
Plasmid#68827PurposeExpresses c-myc-tagged human RAD18DepositorInsertc-myc tagged human RAD18 (RAD18 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
mCherry Codon 59 sgRNA
Plasmid#109431PurposeMLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporterDepositorInsertmCherry codon 59 gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
Non-specific sgRNA
Plasmid#109432PurposeMLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.DepositorInsertNon-specific gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
Empty Target Control
Plasmid#127667PurposeEncodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoterDepositorInsertCRY2-HA-2A-mCherry
UseTagsHAExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
iRFP713-N2-Frankenbody-Flag
Plasmid#175291PurposeVisualize endogeneous loci coupled with ZF probes harboring 10xFLAG tagDepositorInsertanti-FLAG frankenbody fused with iRFP713
UseTagsiRFP713ExpressionMammalianMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-N2-Frankenbody-HA
Plasmid#175292PurposeVisualize endogeneous loci coupled with ZF probes harboring 10xHA tagDepositorInsertanti-HA frankenbody fused with mCherry
UseTagsmCherryExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
cfmTurquoise-N
Plasmid#139284PurposeCys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.DepositorInsertcfmTurquoise
UseTagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC-Hybrid1
Plasmid#173469PurposeN-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1DepositorUseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC-Hybrid2
Plasmid#173470PurposeN-terminal and transmembrane region of PGRMC2 fused to the C-terminal region of PGRMC1DepositorUseTagsFLAGExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC-Hybrid3
Plasmid#173471PurposeN-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2DepositorUseTagsFLAGExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
myc-hRAD18 C207F
Plasmid#68829PurposeExpresses c-myc-tagged human RAD18 with C207F mutationDepositorInsertc-myc tagged human RAD18 with C207F mutation (RAD18 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
myc-hRAD18 dSAP
Plasmid#68830PurposeExpresses c-myc-tagged human RAD18 deleting SAP domainDepositorInsertc-myc tagged human RAD18 deleting SAP domain (RAD18 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
myc-hRAD18 d6BD
Plasmid#68832PurposeExpresses c-myc-tagged human RAD18 deleting hRAD6 binding domainDepositorInsertc-myc tagged human RAD18 deleting hRAD6 binding domain (RAD18 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
LADL Anchor
Plasmid#127664PurposeEncodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoterDepositorInsertdCas9-CIBN-2A-Puro
UseTags3XFLAGExpressionMammalianMutationCas9 (D10A,H840A) mutantPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-mEGFP
Plasmid#175293PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 121 - 140 of 153 results