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Addgene
Showing: 161 - 180 of 334 results

  2. POLG-pcDNA3.1

    Plasmid
    #191487
    Purpose
    Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.
    Depositor
    Hans Zempel
    Insert
    POLG (POLG Human)
    Use
    Tags
    V5 tag, 6X His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Availability
    Academic Institutions and Nonprofits only
    191487-plasmid-map-sequence-id-415364

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  11. rFIXX-bp chain A (IRESneo)

    Plasmid
    #82450
    Purpose
    Chain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.
    Depositor
    Robert Peters
    Insert
    rFIXX-bp chain A
    Use
    Tags
    Polyhistidine
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Availability
    Academic Institutions and Nonprofits only
    82450-plasmid-map-sequence-id-156201

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  12. rFIXX-bp chain B (IRESpuro)

    Plasmid
    #82451
    Purpose
    Chain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.
    Depositor
    Robert Peters
    Insert
    rFIXX-bp chain B
    Use
    Tags
    Biotin
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Availability
    Academic Institutions and Nonprofits only
    82451-plasmid-map-sequence-id-156993

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  13. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

    Plasmid
    #105921
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs
    Depositor
    Marcella Patrick
    Has Service
    AAV2
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Tags
    Expression
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Availability
    Industry, Academic Institutions, and Nonprofits
    105921-plasmid-map-sequence-id-200621

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  14. pST50Trc1-HISDHFR

    Plasmid
    #63935
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    63935-plasmid-map-sequence-id-177341

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  15. pST50Trc1-STRDHFR

    Plasmid
    #63937
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Strep II (STR)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    63937-plasmid-map-sequence-id-177343

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  17. pST50Trc1-HPCDHFR

    Plasmid
    #63939
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    63939-plasmid-map-sequence-id-177345

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  18. pST50Trc1-HISNDHFR

    Plasmid
    #63940
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    63940-plasmid-map-sequence-id-177346

    Enlarge

  19. pST50Trc1-STRNDHFR

    Plasmid
    #63942
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    63942-plasmid-map-sequence-id-177348

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  20. pST50Trc1-FLAGNDHFR

    Plasmid
    #63943
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    63943-plasmid-map-sequence-id-177349

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Showing: 161 - 180 of 334 results