Showing: 61 - 80 of 2137 results
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Plasmid#121175PurposeDonor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell linesDepositorInsertGCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A,K276A, R280A,K284A]
Plasmid#188599PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG], K276A [AAG=>GCG], R280A …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A,K276A]
Plasmid#188596PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG], K276A [AAG=>GCG]PromoterAvailabilityAcademic Institutions and Nonprofits only -
pAWp63-clone32 (Opti-SpCas9)
Plasmid#131736PurposeExpresses Opti-SpCas9 in mammalian cellsDepositorInsertOpti-SpCas9
UseLentiviralTagsFlagExpressionMutationR661A+K1003HPromoterEFSAvailabilityAcademic Institutions and Nonprofits only -
pAWp63-clone3-12 (OptiHF-SpCas9)
Plasmid#131737PurposeExpresses OptiHF-SpCas9 in mammalian cellsDepositorInsertOptiHF-SpCas9
UseLentiviralTagsFlagExpressionMutationQ695A+K848A+E923M+T924V+Q926APromoterEFSAvailabilityAcademic Institutions and Nonprofits only -
pAWp92
Plasmid#157975PurposepBT264-mutH1p-{2xBbsI}-sgRNA scaffold-{MfeI}DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAWp100
Plasmid#157977PurposepBT264-mutmU6p-{2xBbsI}-sgRNA scaffold-{MfeI}DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAWp40
Plasmid#157980PurposepFUGW-EFSp-BFP-{SbfI}DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAWp28-v1 scaffold
Plasmid#157976PurposepBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAWp102-v2 scaffold
Plasmid#157978PurposepBT264-mutmU6p-{2xBbsI}-v2 sgRNA scaffold-{MfeI}DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNM3749
Plasmid#154108PurposeloxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3
UseCre/Lox; RmceTagsExpressionWormMutationPromoternot applicableAvailabilityAcademic Institutions and Nonprofits only -
pBluescript GPX-1
Plasmid#13038DepositorInsertGPX-1 (GPX1 Human)
UseTagsExpressionBacterialMutationAdditional GCG upstream of codon 11. Codon 92 is …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pNM3746
Plasmid#154109PurposeloxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'
UseCre/Lox; RmceTagsExpressionWormMutationPromoternot applicableAvailabilityAcademic Institutions and Nonprofits only -
pNAS1B
Plasmid#61968PurposeDuet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectorsDepositorInsertsmCherry
GFP
UseTagsExpressionBacterialMutationSerine 65 to Glycine (GGT), Serine 72 to Alanine …PromoterPBAD and PLtetOAvailabilityAcademic Institutions and Nonprofits only -
pGL3-p107 1*2*
Plasmid#32387DepositorInsertp107 promoter (Rbl1 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutation1st and 2nd E2F binding sites mutated: GCG-->A…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pGL3-p107 1*
Plasmid#32389DepositorInsertp107 promoter (Rbl1 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutation1st E2F binding site mutation: GCG-->AAAPromoterAvailabilityAcademic Institutions and Nonprofits only -
MYMH 29
Plasmid#138029PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-5AC-GFP
UseTagsExpressionBacterialMutationAAA GCG GAG AAAPromoterAvailabilityAcademic Institutions and Nonprofits only -
MYMH 47
Plasmid#138038PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-8AC-GFP
UseTagsExpressionBacterialMutationAAA GAG GCG AAAPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSUPER-scramble
Plasmid#118349PurposeThis plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.DepositorInsertscrambled shRNA
UseTagsExpressionMammalianMutationWTPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A]
Plasmid#188594PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG]PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 61 - 80 of 2137 results