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pLenti X2 Hygro/pTER shLUC (w607-1)

Plasmid

#20958

Purpose

3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Hygro

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly luciferase shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti X2 Blast/pTER shLUC (w618-1)

Plasmid

#20962

Purpose

3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Blast

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly luciferase shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti X2 Puro/pTER shLUC (w589-1)

Plasmid

#21472

Purpose

3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Puro

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly luciferase shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti X2 Zeo/pTER shLUC (w648-1)

Plasmid

#21561

Purpose

3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Zeo

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly luciferase shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIN X2/pTER shEGFP#1 (w491-1)

Plasmid

#19167

Purpose

Retroviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #2

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti X2 Neo/pTER shEGFP#1 (w21-2)

Plasmid

#17479

Purpose

3rd gen lentiviral eGFP shRNA #1 (H1/TO promoter), 3’LTR insertion, Neo

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #1

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti X2 Neo/pTER shEGFP#2 (w22-2)

Plasmid

#17480

Purpose

3rd gen lentiviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #2

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcBac1-Ma-sfGFP WT

Plasmid

#197567

Purpose

Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP WT
M. alvus Pyl-tRNA (6) (4x copies)

Use

Tags

V5-His6

Expression

Mammalian

Mutation

Promoter

CMV and U6/H1

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcBac1-Mb-sfGFP WT

Plasmid

#197569

Purpose

Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP WT
Pyl-tRNA (4 copies)

Use

Tags

V5-His6

Expression

Mammalian

Mutation

Promoter

CMV and U6/H1

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcBac1-Ma-Acridone-RS1 (A7)

Plasmid

#197566

Purpose

Expression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

M. alvus PylRS - Acd RS1 (A7)
M. alvus Pyl-tRNA (6) (4x copies)

Use

Tags

None

Expression

Mammalian

Mutation

N166A, V168G, W239C

Promoter

CMV and U6/H1

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcBac1-Ma-sfGFP 150TAG

Plasmid

#197568

Purpose

Expression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
M. alvus Pyl-tRNA (6) (4x copies)

Use

Tags

V5-His6

Expression

Mammalian

Mutation

N150TAG

Promoter

CMV and U6/H1

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcBac1-Mb-sfGFP 150TAG

Plasmid

#197570

Purpose

Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
Pyl-tRNA (4 copies)

Use

Tags

V5-His6

Expression

Mammalian

Mutation

N150TAG

Promoter

CMV and U6/H1

Availability

Academic Institutions and Nonprofits only

Enlarge