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Addgene
Showing: 41 - 60 of 62 results

  1. A1-LCD-8F+4Y

    Plasmid
    #178662
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with eight Phe residues removed, four Tyr added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD-8F+4Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    eight Phe residues removed, four Tyr added
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178662-plasmid-map-sequence-id-356201

    Enlarge

  2. A1-LCD-9F+3Y

    Plasmid
    #178663
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, three Tyr added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD-9F+3Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    nine Phe residues removed, three Tyr added
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178663-plasmid-map-sequence-id-356435

    Enlarge

  3. A1-LCD-3R+3K

    Plasmid
    #178669
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with three Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD-3R+3K (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    three Arg residues replaced with Lys
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178669-plasmid-map-sequence-id-356159

    Enlarge

  4. A1-LCD-6R+6K

    Plasmid
    #178670
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD-6R+6K (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    six Arg residues replaced with Lys
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178670-plasmid-map-sequence-id-356194

    Enlarge

  5. A1-LCD+7R+10D

    Plasmid
    #178677
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, ten Glu residues added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD+7R+10D (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Arg residues added, ten Glu residues added
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178677-plasmid-map-sequence-id-356191

    Enlarge

  6. A1-LCD+7R+12D

    Plasmid
    #178678
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD+7R+12D (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Arg residues added, twelve Glu residues add…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178678-plasmid-map-sequence-id-357023

    Enlarge

  7. A1-LCD+7K+12D

    Plasmid
    #178679
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Lys residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD+7K+12D (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Lys residues added, twelve Glu residues add…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178679-plasmid-map-sequence-id-356398

    Enlarge

  8. A1-LCD-2R-2K+3D

    Plasmid
    #178680
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues removed, two Lys removed, three Asp added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD-2R+2K+3D (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    two Arg residues removed, two Lys added, three As…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178680-plasmid-map-sequence-id-356258

    Enlarge

  9. A1-LCD-4R-2K+5D

    Plasmid
    #178681
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with four Arg residues removed, two Lys removed, five Asp added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD-4R+2K+5D (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    four Arg residues removed, two Lys added, five As…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178681-plasmid-map-sequence-id-356295

    Enlarge

  10. A1-LCD-14N-4Q+18G

    Plasmid
    #178694
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues removed, four Asn removed, replaced with eighteen Gly (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD-14N-4Q+18G (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    fourteen Gln residues removed, four Asn removed, …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178694-plasmid-map-sequence-id-356315

    Enlarge

  12. A1-LCD-30G+30S+7F-7Y

    Plasmid
    #178686
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD-30G+30S+7F-7Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    thirty Gly residues replaced with Ser, seven Tyr …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178686-plasmid-map-sequence-id-357017

    Enlarge

  13. A1-LCD-30G+30S-12F+12Y

    Plasmid
    #178687
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD-30G+30S-12F+12Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    thirty Gly residues replaced with Ser, twelve Phe…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178687-plasmid-map-sequence-id-357019

    Enlarge

  14. A1-LCD-20G+20S+7F-7Y

    Plasmid
    #178688
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD-20G+20S+7F-7Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty Gly residues replaced with Ser, seven Tyr …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178688-plasmid-map-sequence-id-356407

    Enlarge

  15. A1-LCD-20G+20S-12F+12Y

    Plasmid
    #178689
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD-20G+20S-12F+12Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty Gly residues replaced with Ser, twelve Phe…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178689-plasmid-map-sequence-id-356275

    Enlarge

  16. A1-LCD+23G-23S+7F-7Y

    Plasmid
    #178690
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, seven Tyr replaced with Phe (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD+23G-23S+7F-7Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly, seve…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178690-plasmid-map-sequence-id-356145

    Enlarge

  17. A1-LCD+23G-23S-12F+12Y

    Plasmid
    #178691
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Insert
    A1-LCD+23G-23S-12F+12Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly, twel…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178691-plasmid-map-sequence-id-356195

    Enlarge

  19. pAAV-U6-racRNA-A

    Plasmid
    #200824
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Availability
    Academic Institutions and Nonprofits only
    200824-plasmid-map-sequence-id-396714

    Enlarge

  20. pAAV-U6-racRNA-B

    Plasmid
    #200825
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Availability
    Academic Institutions and Nonprofits only
    200825-plasmid-map-sequence-id-396771

    Enlarge

Showing: 41 - 60 of 62 results