We narrowed to 542 results for: prime editing

1. PrimeRoot.v2C-Cre

   Plasmid

   #201739

   Purpose

   For DNA insertion test in protoplast

   Depositor

   Caixia Gao

   Article

   Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   ePPE,Cre
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PrimeRoot.v2C-FLP

   Plasmid

   #201740

   Purpose

   For DNA insertion test in protoplast

   Depositor

   Caixia Gao

   Article

   Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   ePPE,FLP
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDAS12395_PEAR-GFP_2.0-preedited

   Plasmid

   #177185

   Purpose

   PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDAS12124_PEAR-GFP-preedited

   Plasmid

   #177179

   Purpose

   PEAR-GFP control plasmid with split EGFP and intact 5' splice site

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. XLone-PE2

   Plasmid

   #136463

   Purpose

   Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment

   Depositor

   Xiaojun Lian

   Insert

   PE2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-TREG3G-PE2-rtTA3G-P2A-eGFP

   Plasmid

   #196971

   Purpose

   Piggybac vector with doxycyclin-inducible prime editor for mammalian cells

   Depositor

   Leopold Parts

   Inserts

   Cas9-RT
   rtTA3G-P2A-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK and TREG3G

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBtet-RB-PE2

   Plasmid

   #173903

   Purpose

   SB-transposon with inducible expression of SpCas9 PE2

   Depositor

   Ronald Cohn

   Insert

   PE2
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBbi-RB-PE2

   Plasmid

   #173902

   Purpose

   SB-transposon with constitutive expression of SpCas9 PE2

   Depositor

   Ronald Cohn

   Insert

   PE2
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PE_CO

   Plasmid

   #190335

   Purpose

   Prime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cells

   Depositor

   Rasmus Bak

   Article

   Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

   Insert

   PE2 with codon-optimized reverse transcriptase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-PEmax-SpG

    Plasmid

    #211549

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pePEmax-pPuro

    Plasmid

    #215688

    Purpose

    Cloning vector for Uni-Vector prime editing system

    Depositor

    Kou-Juey Wu

    Article

    Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

    Insert

    PEmax
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPE3-pPuro

    Plasmid

    #215722

    Purpose

    Cloning vector for Uni-Vector prime editing system

    Depositor

    Kou-Juey Wu

    Article

    Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

    Insert

    PE2
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid

    #217800

    Purpose

    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR-PE-bacteria

    Plasmid

    #172715

    Purpose

    Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    Cas9n-linker-M-MLV2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H840A of Cas9

    Promoter

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-PEmax-SpG-P2A-hMLH1dn

    Plasmid

    #211550

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG-P2A-hMLH1dn
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEntr_PE2

    Plasmid

    #149548

    Purpose

    Gateway entry clone with PE2 enzyme (with stop codon)

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    PE2
    

    Use

    CRISPR; Gateway shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PE2-P2A-PuroR

    Plasmid

    #196962

    Purpose

    Expresses PE2 with puromycin resistance

    Depositor

    Leopold Parts

    Insert

    PE2-P2A-Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAVS1-iPEmax-Hygro

    Plasmid

    #214020

    Purpose

    inducible PEmax system for controllable prime editing; this plasmid is used to insert PEmax prime editor in one allele of AAVS1 locus

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PEmax
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAVS1-iPE2-Hygro

    Plasmid

    #214019

    Purpose

    inducible PE2 system for controllable prime editing; this plasmid is used to insert PE2 prime editor in one allele of AAVS1 locus

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC57-CmYLCV

    Plasmid

    #205245

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCV

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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