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Showing: 1 - 20 of 50 results

  7. pCW-eGFP-FHA

    Plasmid
    #114129
    Purpose
    Lentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin
    Depositor
    Daniel Durocher
    Article
    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:
    Insert
    RNF8 FHA domain (amino acids 2-160) (RNF8 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    aa 2-160
    Promoter
    TRE promoter, Tet ON
    Availability
    Academic Institutions and Nonprofits only
    114129-plasmid-map-sequence-id-220207

    Enlarge

  8. pCW-eGFP-FHA-SHLD2

    Plasmid
    #114121
    Purpose
    Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatin
    Depositor
    Daniel Durocher
    Article
    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:
    Insert
    SHLD2 (SHLD2 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    RNF8 FHA domain (aa 2-160) and eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TRE promoter, Tet ON
    Availability
    Academic Institutions and Nonprofits only
    114121-plasmid-map-sequence-id-220199

    Enlarge

  9. pCW-eGFP-FHA-SHLD2-m1

    Plasmid
    #114122
    Purpose
    Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin
    Depositor
    Daniel Durocher
    Article
    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:
    Insert
    SHLD2 (m1 mutant) (SHLD2 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    RNF8 FHA domain (aa 2-160) and eGFP
    Expression
    Mammalian
    Mutation
    W489A, F494A, W495A mutations introduced
    Promoter
    TRE promoter, Tet ON
    Availability
    Academic Institutions and Nonprofits only
    114122-plasmid-map-sequence-id-220204

    Enlarge

  12. GST-TRIM24

    Plasmid
    #28146
    Depositor
    Michelle Barton
    Insert
    TRIM24 (TRIM24 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  14. GST-TRIM24+TEV

    Plasmid
    #28147
    Depositor
    Michelle Barton
    Insert
    TRIM24 (TRIM24 Human)
    Use
    Tags
    GST and TEV cleavage site
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  15. GST-TRIM24ΔRING

    Plasmid
    #28148
    Depositor
    Michelle Barton
    Insert
    TRIM24 (TRIM24 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Deletion of the RING domain: aa 1-139
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  17. Flag-TRIM24-F979A/N980A

    Plasmid
    #28142
    Depositor
    Michelle Barton
    Insert
    TRIM24 (TRIM24 Human)
    Use
    Tags
    FLAGX2
    Expression
    Mammalian
    Mutation
    F979A and N980A
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  18. Flag-TRIM24-F869D

    Plasmid
    #28141
    Depositor
    Michelle Barton
    Insert
    TRIM24 (TRIM24 Human)
    Use
    Tags
    FLAGX2
    Expression
    Mammalian
    Mutation
    A489V, F869D; aa 308 and 309 mutated for shRNA re…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  19. TIF1A

    Plasmid
    #74647
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TIF1A (TRIM24 Human)
    Use
    Tags
    His-6-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  20. Flag-TRIM24-F979A/N980A shRescue

    Plasmid
    #28143
    Depositor
    Michelle Barton
    Insert
    TRIM24 (TRIM24 Human)
    Use
    Tags
    FLAGX2
    Expression
    Mammalian
    Mutation
    A489V, F979A and N980A; aa 308 and 309 mutated fo…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 50 results