We narrowed to 111 results for: addgene

1. Mouse sgRNA library Gouda in pRDA_118 backbone

   Pooled Library

   #136987

   Purpose

   Expresses sgRNAs targeting mouse genes

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Expression

   Mammalian

   Species

   Mus musculus

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

2. Human messenger-RBP sgRNA library

   Pooled Library

   #168791

   Purpose

   CRISPR library targets sub-pool of human mRNA binding proteins

   Depositor

   Martin Turner

   Article

   Turner et al CRISPR J. 2021 Jun;4(3):427-437. doi: 10

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

3. p11-3’_8xN_PAM-site1 (RTW554)

   Pooled Library

   #160132

   Purpose

   Plasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

4. sgRNA pool for Streptococcus pneumoniae

   Pooled Library

   #170432

   Purpose

   CRISPR library targeting S. pneumoniae D39V

   Depositor

   Jan-Willem Veening

   Article

   Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

   Expression

   Bacterial

   Use

   CRISPR

   Available since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

5. p11-5’_10xN_PAM-site4 (RTW574)

   Pooled Library

   #160135

   Purpose

   Plasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

6. p11-3’_8xN_PAM-site2 (RTW555)

   Pooled Library

   #160133

   Purpose

   Plasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

7. Mouse messenger-RBP sgRNA library

   Pooled Library

   #169082

   Purpose

   CRISPR library targeting mouse genes

   Depositor

   Martin Turner

   Article

   Turner et al eLife. 2022 Apr 22;11. pii: 72313. doi:

   Expression

   Mammalian

   Species

   Mus musculus

   Use

   CRISPR and Lentiviral

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

8. Human Metabolic Gene CRISPRa sgRNA library

   Pooled Library

   #187080

   Purpose

   Lentiviral CRISPRa library targeting human metabolic genes for activation of gene expression

   Depositor

   Kivanc Birsoy

   Article

   Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   Lentiviral

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

9. RLC11

   Pooled Library

   #163955

   Purpose

   RLC11 pooled library

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Expression

   Bacterial

   Use

   CRISPR

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

10. RLC12

    Pooled Library

    #163954

    Purpose

    RLC12 pooled library

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Expression

    Bacterial

    Use

    CRISPR

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

11. Mouse Metabolic Gene CRISPRa sgRNA library

    Pooled Library

    #187081

    Purpose

    Lentiviral CRISPRa library targeting mouse metabolic genes for activation of gene expression

    Depositor

    Kivanc Birsoy

    Article

    Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    Lentiviral

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

12. Human Genome-Wide Reduced Double-gRNA library

    Pooled Library

    #137999

    Purpose

    This gRNA library targets the human coding genome, aimed to reduce the scale of genome-wide perturbation screens up to fivefold compared to conventional methods, without sacrificing performance.

    Depositor

    Leopold Parts

    Article

    Peets et al bioRxiv 859652

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

13. lentiGuide-Puro-pKO-library

    Pooled Library

    #190744

    Purpose

    CRISPR/Cas9 paired-guide RNA library to delete proximal poly(A) sites in mouse melanoma.

    Depositor

    Robert Bradley

    Article

    Gabel et al Nat Commun. 2024 Feb 1;15(1):959. doi: 1

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

14. KSHV Tiling Library

    Pooled Library

    #180272

    Purpose

    sgRNA library tiling entire ~154 kb of the BAC16 Kaposi’s Sarcoma-associated Herpes viral genome. A guide every ~8 bp allows pooled screening of viral genome.

    Depositor

    Britt Glaunsinger

    Article

    Morgens et al PLoS Pathog. 2022 Jan 18;18(1):e1010236.

    Expression

    Mammalian

    Species

    Kaposi’s sarcoma-associated herpes virus

    Use

    Lentiviral

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

15. Human Whole Genome sgRNA iBAR Library

    Pooled Library

    #140633

    Purpose

    CRISPR pooled screen at high MOI by the embedded internal barcodes

    Depositor

    Wensheng Wei

    Article

    Zhu et al Genome Biol. 2019 Jan 24;20(1):20. doi:

    Use

    CRISPR and Lentiviral

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

16. mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP

    Pooled Library

    #197349

    Purpose

    To allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2.

    Depositor

    Rebecca Voorhees, Jonathan Weissman

    Article

    Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

17. Humagne Set C

    Pooled Library

    #172650

    Purpose

    Set C contains best-ranked guides 1 and 4.

    Depositor

    John Doench, David Root

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Expression

    Mammalian

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

18. DNA repair library with 1,573 sgRNAs

    Pooled Library

    #177663

    Purpose

    CRISPR-interference (CRISPRi) library of 1,573 sgRNAs targeting 476 genes encoding factors involved in DNA repair and associated processes

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Species

    Homo sapiens

    Use

    CRISPR

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

19. Humagne Set D

    Pooled Library

    #172651

    Purpose

    Set D contains best-ranked guides 2 and 3.

    Depositor

    John Doench, David Root

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Expression

    Mammalian

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

20. Inzolia library in pRDA_052

    Pooled Library

    #209551

    Purpose

    The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

    Depositor

    John Doench

    Article

    Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

    Use

    CRISPR and Lentiviral

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only