We narrowed to 4 results for: pam

1. ALL-PAM sensor Library

   Pooled Library

   #179219

   Purpose

   10 human and mouse sgRNAs each where for each sgRNA the tethered target site has 1 of 64 possible 3 bp PAM sequences.

   Depositor

   Lukas Dow

   Article

   Sanchez-Rivera et al Nat Biotechnol. 2022 Feb 14. pii: 10.103

   Expression

   Mammalian

   Use

   Lentiviral

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

2. p11-3’_8xN_PAM-site1 (RTW554)

   Pooled Library

   #160132

   Purpose

   Plasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

3. p11-5’_10xN_PAM-site4 (RTW574)

   Pooled Library

   #160135

   Purpose

   Plasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

4. p11-3’_8xN_PAM-site2 (RTW555)

   Pooled Library

   #160133

   Purpose

   Plasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only