We narrowed to 59 results for: cag promoter

...Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1 with a CAG promoter exhibits high specificity for... 59462  AAV2  pAAV-CAG-tdTomato (codon diversified) 37825  AAV8*, AAV9*  pAAV-CAG-GFP 50465  AAV8*...pAAV-FLEX-tdTomato pAAV-GFAP104-mCherry pAAV-mDlx-NLS-mRuby2 CAG-NLS-GFP pAAV-hSyn-mCherry Read on to learn more...pAAV-FLEX-tdTomato, pAAV-GFAP104-mCherry, pAAV-mDlx-NLS-mRuby2, CAG-NLS-GFP,and pAAV-hSyn-mCherry at Addgene. Viral vectors...hM4D(Gi)-mCherry (CD68 is a  microglia/macrophage promoter) 121539  AAV5  pOTTC1596 - pAAV SYN1 HA-hM3D...

...Synapsin promoter directs broad, neuronal expression. AAV pCAG-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (104493-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (104495-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (104496...serotypes. This collection is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465... to give scientists access to a wide array of promoters and fluorophores for validating and testing purposes...-EGFP (112677-AAV5 and 112677-AAVrg): The EF1a promoter directs strong, ubiquitous expression. This is...

...work with many cell types, as CAG is a ubiquitous and constitutive promoter. Use this for doxycycline inducible...pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes pAAV-CAG-tTA AAV2...repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser from the Gradinaru lab. This...pAAV-Ef1a-fDIO-ChrimsonR-tdTomato AAV9 Controls Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls...

...protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter...Human Synapsin-1 promoter. Good for neuronal expression. hSynap-FLEX: Synapsin-1 promoter, FLEXed for expression...Synapsin-1 promoter, FLP-dependent expression GFAP: Glial fibrillary acidic protein (promoter). Good for...Podgorski Lab has provided a brief breakdown of the promoter systems you can use to express the iGluSnFR3 variants...

...potentials, is now offered under control of the CAG promoter for ubiquitous expression. (For more details...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE 104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714...collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471 AAV1, AAV5 pAAV-Ef1a-fDIO...

...pyogenes (Sp) Cas9 from a Cbh promoter. Cbh is a modified version of CAG promoter that the Zhang lab has tested...imporant is that, upstream of the Cbh promoter, this plasmid has a U6 promoter followed by BbsI restriction sites...

...interest. Promoter The promoter drives the expression of the AAV transgene. On a plasmid map, promoters are ...location. Promoters can also drive different levels of expression. For example, CAG is a strong promoter that...synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer plasmids have a promoter, but...are upstream or 5’ of the gene they control. Promoters can be organism or tissue type-specific, which can...nuclear export of RNA and RNA translation, and promotes RNA transcript longevity. The polyA is located...for the perfect AAV tool Learn about synthetic promoter AAVs for cell-type expression in retinal cells...

... trying to pick your perfect promoter! Eukaryotic Promoters   Promoter   Primarily used for RNA     ...commonly used in promoter hybrids. CAG General expression mRNA Strong hybrid mammalian promoter Constitutive.... In practice, the term "promoter" describes the combination of the promoter (RNA polymerase binding site...recognize and bind to specific promoter elements. This means that the promoter present in your plasmid backbone...RNAP II promoter, whereas small RNAs (such as shRNA) are transcribed from the RNAP III promoters. This ...post on viral vector parts. Promoter specificity Aside from choosing a promoter based on type of RNA transcript...cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...

...rat neurons over other promoters, including ubiquitous ones such as EF-1α and CAG. Figure 2: Dual...plasmids used to express CRE under neuron-specific promoters (aCaMKII and hSyn). Created with BioRender.com...plasmids used to express Cre under neuron-specific promoters, including calmodulin-dependent kinase II alpha...excitatory neurons) and Synapsin-1 (pan-neuronal) promoters (Figure 1b), and sgRNAs can be found here. Neuron-optimized...expression is mediated by the synapsin 1 (SYN) promoter, which was superior in its ability to induce CRISPRa...consequently regulates neuronal physiology by promoting the development of neurons and synapses as well...cells to determine which transcription factors promote neuronal differentiation (Liu et al., 2018). The...

..., the CAG promoter, a hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter, and rabbit... tags and fusion proteins Finally, we confirm promoters, tags, fusion proteins, and selectable markers...

...Plasmid Serotype Name Depositor 75470 AAV1 pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry Connie Cepko 83898 ...activated, this DREADD acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor...more accurate cell and tissue targeting. While promoters and AAV serotypes can sometimes be used to target...

...neuron-specific expression (hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved version ...

...your experiment site or circuit can occur. Promoters The promoter controls how the gene of interest will ...selective, or inducible.  Ubiquitous promoters  These often-strong promoters offer the most flexibility for ...large number of different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression...vitro) or months (in vivo).  Selective promoters Selective promoters, such as synapsin, glial fibrillary ...International License.   Inducible promoters  This type of promoter allows you to decide when to turn ...three key variables of an AAV vector: serotype, promoter, and titer.     Figure 1: Flow chart depicting...depicting a) steps for selecting serotypes and promoters to test in your AAV system and b) steps for testing...

...the injection site. Test the promoter It is also essential that the promoter be thoroughly tested in your...your cells.  Some promoters, such as CAG are very strong and may require less viral particles or relatively...cell-type specific” promoter, such as the truncated tyrosine hydroxylase (TH) promoter for catecholamine...open reading frame is inverted in relation to the promoter, hence the name, double-floxed inverted open-reading...what can we do about it?  Test the serotype and promoter The first step is to know how the AAV vector you...includes a thorough testing of both the serotype and promoter with in vivo experiments to determine the optimal...expressing TH protein indicating that there is promoter activity. Additionally, some neurons express low...

...Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral vector...under the control of Murine Stem Cell retrovirus promoter for mammalian gene knockdown. Vector provided ...

...fluorescent protein EYFP from the CAG promoter Viviana Gradinaru 104055-AAV2 pAAV-CAG-eYFP An AAV genome that ubiquitously...fluorescent protein eYFP from the CAG promoter Viviana Gradinaru 104055-AAV5 pAAV-CAG-eYFP An AAV genome that ubiquitously...fluorescent protein eYFP from the CAG promoter Viviana Gradinaru 104055-AAVrg pAAV-CAG-eYFP An AAV genome that ...fluorescent protein eYFP from the CAG promoter Viviana Gradinaru 104055-PHPeB pAAV-CAG-eYFP An AAV genome that ...fluorescent protein eYFP from the CAG promoter Viviana Gradinaru 104061-PHPeB CAG-NLS-GFP AAV expression of nuclear...expression of dLight1.1 under CAG promoter Lin Tian 111067-AAV5 pAAV-CAG-dLight1.1 To generate Adeno-Associated...Li 158757-AAV8 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by...

...Gradinaru 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE... PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP, Cre-dependent ...Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden MaCPNS2...Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-...Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-...Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden AAV9...

... pAAV-CAG-eYFP CAG EYFP Constitutive 2, 5, rg*, PHP.eB Viviana Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP...116869 pAAV-CAG-H2B-GFP CAG H2B-GFP Constitutive rg* Loren Looger 116870 pAAV-CAG-H2B tdTomato CAG H2B-tdTomato... Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive ...-EGFP CAG EGFP Cre dependent 1 Ian Wickersham 104052 pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1 Viviana...51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent 1, 2, 5, 8, 9, rg* Hongkui Zeng 59331 pAAV-CAG-FLEX-EGFP...PHP.V1 CAP-B10 CAP-B22 MaCPNS1 MaCPNS2 AAV9-X1.1 Promoter CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants Synapsin...EYFP Constitutive 1 Brandon Harvey 59462 pAAV-CAG-tdTomato CAG tdTomato Constitutive 1, 2, 5, 8, 9, rg*, ...

...Positive Control CAG promoter pEMS1293 cre N/A CAG promoter pEMS1164 EGFP/cre N/A CAG promoter pEMS1114 EGFP...cre/NLS N/A CAG promoter pEMS1157 EGFP/NLS N/A CAG promoter pEMS1277 EGFP/NLS N/A CAG promoter pEMS1294 ...Collections Pleiades Promoter Plasmids Pleiades Promoter Project The Pleiades Promoter Project (Link opens...pEMS1294 intron-lacZ/NLS N/A CAG promoter pEMS1488 intron-lacZ/NLS References Portales-Casamar, E., Swanson, ...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective expression...mouse brain through these human MiniPromoters. Browse the Pleiades Promoter Plasmids using the table below...Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters MiniPromoter Source Gene Construct Reporter Ple2 ADORA2A...

... Zea mays ubiquitin promoter. Paul Schulze-Lefert 83282 pAAV-CAG-RLuc Renilla CAG AAV expression of Renilla...of 5' promoter/enhancer regions Joshua Mendell 60323 pGL4.23-GW Firefly Insertion of 5' promoter/enhancer...luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc Firefly CAG AAV expression of firefly luciferase Mark...Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc Renilla CAG Vector for packaging self-complementary ...tool for scientists. Regulatory elements such as promoters, enhancers and untranslated regions, or shRNA ...Jorge Ferrer 16539 pBV-Luc Firefly Insertion of 5' promoter/enhancer regions Bert Vogelstein 12178 pIS0 Firefly... Renilla luciferase under the control of a CMV promoter is present for normalization William Kaelin 71248...

...Wilson 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana Gradinaru...Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana Gradinaru...Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana Gradinaru...Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana Gradinaru...Viviana Gradinaru 163489-AAV1 AiP1051-pAAV-Synapsin promoter-oNigri-WPRE-hGHpA Direct-expressing oNigri AAV...

... AAV-PHP.eB, when paired with the ubiquitous CAG promoter, is relatively biased toward L5 and inhibitory...quite pronounced, even when using a ubiquitous CAG promoter. Spatial transcriptomics shows that its tropism...transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target sites, and recombination...over systemic delivery. Otherwise, cell-specific promoters and enhancers (Mich et al., 2021) can help restrict...optogenetic and chemogenetic tools. Dual expression Promoters, miRNAs, enhancers, and switches all take up precious...particular experiment is determined by the capsid and promoter/enhancer strengths in the cell type of interest...candidate regulatory elements such as enhancers and promoters for cell type-specific targeting (Chan et al.,...

...′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately 500 bp genomic fragment containing...cassette. (b) pCAG-EGxxFP target plasmid and pX330-sgRNA plasmid (Mashiko et al. 2013). The pCAG-EGxxFP target...cloned directionally into the BbsI site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330...Once amplified, you can insert this region into a pCAG-EGXXFP plasmid using standard cloning techniques...of transfecting HEK293T cells with your modified pCAG-EGXXFP plasmid along with the individual gRNAs in...Remember the primers you designed to generate your pCAG-EGXXFP plasmid? They are the perfect primer sets...

...128485 pAAV-CAG-iSeroSnFR-Nlgn CAG iSeroSnFR none Constitutive 9 Lin Tian 128486 pAAV-CAG-FLEx-iSeroSnFR...5-HT Sensors GRAB_5-HT Lactate Sensors LiLac Promoter CAG CaMKII Dlx EF1a GFAP/GfaABC1D Synapsin Other..., 9, rg* GENIE Project 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, ...Constitutive 1, 9 Loren Looger 179254 AAV-CAG-jGCaMP8f-WPRE CAG jGCaMP8f none Constitutive 1, 9, rg* Loren..., 9, rg* GENIE Project 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent 1, 9, rg*...Constitutive 1, 9 Loren Looger 179256 AAV-CAG-jGCaMP8s-WPRE CAG jGCaMP8s none Constitutive 1, 9, rg* Loren..., 9, rg* GENIE Project 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE CAG jGCaMP8m none Cre-dependent 1, 5, 9, ...

...AAV-CAG-hChR2-H134R-tdTomato CAG ChR2/H134R tdTomato Constitutive rg* Karel Svoboda 75470 pAAV-CAG-FLEXFRT-ChR2...Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus ...stGtACR iC++ OPN3 PdCO PPO Bidirectional BiPOLES Promoter CAG CaMKII CBA Dlx E2 regulatory element EF1a/nEF... 2, 5, 9 Karl Deisseroth 127090 pAAV-CAG-DIO-ChR2(H134R)-eYFP CAG ChR2/H134R EYFP Cre dependent PHPeB ...Constitutive 8 Edward Boyden 130909 AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] CAG ChrimsonR tdTomato Flp dependent...9 Christopher Harvey 108912 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 CAG ChroME (soma-targeted) mRuby3 Cre...dependent 9 Hillel Adesnik 170163 pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3 CAG ChroME2s mRuby3 Cre dependent 1...

...Expression System Activity Serotype PI 99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off...tdTomato. 8 Massimo Scanziani 34910 paavCAG-pre-mGRASP-mCerulean CAG-driven, constitutive Expression of...connectivity mapping. 5 Jinhyun Kim 34912 paavCAG-post-mGRASP-2A-dTomato CAG-driven, constitutive Expression of...Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator...pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of the tet-off...adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner. 1 Don Arnold 124364... with cysteine-free GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev 200778 AAV-∆Hapln1 hSyn-...

...the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter. Truncations...directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed for in vitro...DNA sequences within core promoter regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into...fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence to drive efficient TALEN ...galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built...double-transfected mammalian cells. In addition, a T7 promoter was included for generating TALEN mRNAs through...expression. The vectors listed below have the EF1α promoter for efficient mammalian cell expression, and encode...

...Wilson 116869 pAAV-CAG-H2B-GFP CAG GFP Control Loren Looger 116870 pAAV-CAG-H2B tdTomato CAG tdTomato Control...Name Promoter Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE... AAV-CAG-jGCaMP8f-WPRE EF1a GCaMP8f Calcium sensor Loren Looger 179255 AAV-CAG-jGCaMP8m-WPRE CAG GCaMP8m...Cre-dependent Control Bryan Roth 59462 pAAV-CAG-tdTomato CAG tdTomato Control Edward Boyden 50457 pAAV-...Dlx dTomato Control Gordon Fishell 104055 pAAV-CAG-eYFP CAG EYFP Control Viviana Gradinaru 112677 pOTTC1032...expression Recombinases Ian Wickersham 183412 pAAV-CAG-FlpO CAG Flpo expression Recombinases Janelia Viral Tools...Calcium sensor Douglas Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG GCaMP7s, Cre-dependent Calcium sensor...

...-DOG-NW EF1a none 1 Connie Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia Viral Tools 183535 pAAV-...information about these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40...AAV.TBG.PI.Cre.rBGe TBG none 8 James M. Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)... Syn none 5, rg* Hongkui Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)...Research Program Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV...EF1a none 1, PHPeB Hongkui Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre...

... Boyden ) and direct GFP expression from the CAG promoter. For information about each catalog item, including...example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV...direct EGFP expression from the human synpasin promoter. For information about each catalog item, including...