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  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...92247 pJOP-HTT-HR30Q HTT copGFP E1Fa Huntington's Mahmoud Pouladi 92248 pJOP-HTT-HR45Q HTT copGFP E1Fa...92249 pJOP-HTT-HR65Q HTT copGFP E1Fa Huntington's Mahmoud Pouladi 92250 pJOP-HTT-HR81Q HTT copGFP E1Fa...His6-Ssp-Httex1-7Q HTT His T7 Huntington's Hilal Lashuel 84348 pTWIN1-His6-Ssp-Httex1-15Q HTT His T7 Huntington's...His6-Ssp-Httex1-23Q HTT His T7 Huntington's Hilal Lashuel 84350 pTWIN1-His6-Ssp-Httex1-29Q HTT His T7 Huntington's...His6-Ssp-Httex1-37Q HTT His T7 Huntington's Hilal Lashuel 84352 pTWIN1-His6-Ssp-Httex1-43Q HTT His T7 Huntington's...Garcia 157860 61427-sgHtt_6 HTT U6 Huntington's Xinyu Zhao 157861 61427-sgHtt_10 HTT U6 Huntington's Xinyu...-U6-sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6,...

  2. Neurodegeneration Research Collection

    Type
    Collection
    ...series within the huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin proteins...neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. New and Noteworthy...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...Gene Expression . Curr Gene Ther, 16 (3), 156–167. https://doi.org/10.2174/1566523216666160524144041 (Link.... Proc Natl Acad Sci U S A, 89 (12), 5547–5551. https://doi.org/10.1073/pnas.89.12.5547 (Link opens in...mammalian cells . Science, 268 (5218), 1766–1769. https://doi.org/10.1126/science.7792603 (Link opens in...dynamic range . Hum Gene Ther, 22 (2), 166–176. https://doi.org/10.1089/hum.2010.099 (Link opens in a ...transregulators . BioTechniques, 37 (4), 546–550. https://doi.org/10.2144/04374BM04 (Link opens in a new...background expression . BMC Biotechnol, 10 , 81. https://doi.org/10.1186/1472-6750-10-81 (Link opens in...inducible system . Mol Cell Biol, 14 (3), 1669–1679. https://doi.org/10.1128/mcb.14.3.1669-1679.1994 (Link ...

  4. Structural Genomics Consortium Plasmids

    Type
    Collection
    ..., AmpR Related Resources SGC: http://www.thesgc.org/ SGC Toronto: https://www.thesgc.org/scientists/groups...groups/toronto SGC Oxford: http://www.sgc.ox.ac.uk/ Protein Database (PDB): http://www.pdb.org Request Plasmids...

  5. Gibson Assembly Protocol

    Type
    Protocol
    ...hundred kilobases. Nature Methods , 6(5), 343–345. https://doi.org/10.1038/nmeth.1318 (Link opens in a new...mitochondrial genome. Nature Methods , 7(11), 901–903. https://doi.org/10.1038/nmeth.1515 (Link opens in a new...Enhancement for Gibson Isothermal Assembly. bioRxiv . https://doi.org/10.1101/2020.06.14.150979 (Link opens ...

  8. Lentiviral Vector Guide

    Type
    Guide
    ...Pseudotyping. Current Gene Therapy , 5 (4), 387–398. https://doi.org/10.2174/1566523054546224 PMID: 16101513...System. Journal of Virology , 72 (11), 8463–8471. https://doi.org/10.1128/jvi.72.11.8463-8471.1998 PMID:...of lentiviral vectors. Viruses , 3 (2), 132–159. https://doi.org/10.3390/v3020132 PMID: 22049307 Ghaleh.... Biomedicine & Pharmacotherapy , 128 , 110276. https://doi.org/10.1016/j.biopha.2020.110276 PMID: 32502836... Methods & Clinical Development , 21 , 451–465. https://doi.org/10.1016/j.omtm.2021.03.018 PMID: 33981779... Methods & Clinical Development , 20 , 792–800. https://doi.org/10.1016/j.omtm.2020.10.012 PMID: 33768124...Integration Targeting. PLoS ONE , 2 (12), e1340. https://doi.org/10.1371/journal.pone.0001340 PMID: 18092005...

  9. Adenovirus Guide

    Type
    Guide
    ...strategies . Current Gene Therapy, 11 (4), 307–320. https://doi.org/10.2174/156652311796150372 (Link opens...Signal Transduction and Targeted Therapy, 6 (1), 53. https://doi.org/10.1038/s41392-021-00487-6 (Link opens...virus vaccine . Scientific Reports, 10 (1), 3613. https://doi.org/10.1038/s41598-020-60238-5 (Link opens...Vaccines & Immunotherapeutics, 13 (12), 3020–3032. https://doi.org/10.1080/21645515.2017.1383575 (Link opens...challenge . Journal of Virology, 85 (9), 4222–4233. https://doi.org/10.1128/JVI.02407-10 (Link opens in a ... Expert Review of Vaccines, 14 (10), 1347–1357. https://doi.org/10.1586/14760584.2015.1077122 (Link opens...the United States of America, 95 (5), 2509–2514. https://doi.org/10.1073/pnas.95.5.2509 (Link opens in ...

  10. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...nervous system . Molecular Therapy, 10 (2), 302–317. https://doi.org/10.1016/j.ymthe.2004.05.024 (Link opens...systems . Nature Neuroscience, 20 (8), 1172–1179. https://doi.org/10.1038/nn.4593 (Link opens in a new window...comparison . Molecular Therapy, 4 (4), 383–391. https://doi.org/10.1006/mthe.2001.0456 (Link opens in ...therapy . Current Gene Therapy, 3 (4), 281–304. https://doi.org/10.2174/1566523034578285 (Link opens in...production . Journal of Virology, 77 (8), 4881–4887. https://doi.org/10.1128/jvi.77.8.4881-4887.2003 (Link ...transfer . Pharmaceutical Research, 25 (3), 489–499. https://doi.org/10.1007/s11095-007-9431-0 (Link opens ...Cytokine & Growth Factor Reviews, 80 , 109–120. https://doi.org/10.1016/j.cytogfr.2024.09.003 (Link opens...

  11. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...integration. Journal of Virology , 88 (8), 4504–4513. https://doi.org/10.1128/jvi.00011-14 PMID: 24501411 Gilroy...in cancer genomics. PLoS ONE , 11 (4), e0154070. https://doi.org/10.1371/journal.pone.0154070 PMID: 27097319...promoters. Nucleic Acids Research , 42 (7), 4257–4269. https://doi.org/10.1093/nar/gkt1399 PMID: 24464997 Maetzig...technology and application. Viruses , 3 (6), 677–713. https://doi.org/10.3390/v3060677 PMID: 21994751 Mekkaoui... Methods & Clinical Development , 28 , 116–128. https://doi.org/10.1016/j.omtm.2022.12.006 PMID: 36620071...chimeric GALV envelope. Viruses , 13 (8), 1471. https://doi.org/10.3390/v13081471 PMID: 34452336 Schnierle...National Academy of Sciences , 94 (16), 8640–8645. https://doi.org/10.1073/pnas.94.16.8640 PMID: 9238030 ...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...more information on reagents and protocols, visit https://clover.caltech.edu. Figure adapted from Challis... Review of Neuroscience. 2022 Jul 8;45:447-469. https://doi.org/10.1146/annurev-neuro-111020-100834 Challis...in rodents. Nature Protocols;14, 379–414 (2019). https://doi.org/10.1038/s41596-018-0097-3 More resources...Using AAV-PHP.S. eNeuro,9(3), ENEURO.0373-21.2022. https://doi.org/10.1523/ENEURO.0373-21.2022 Brown, D.,...Sequencing. Frontiers in immunology, 12, 730825. https://doi.org/10.3389/fimmu.2021.730825 Campos LJ, Arokiaraj...system. Current Research in Neurobiology, 4, 100086. https://doi.org/10.1016/j.crneur.2023.100086. Challis... Annual Review of Neuroscience, 45(1), 447–469. https://doi.org/10.1146/annurev-neuro-111020-100834 Chan...

  13. Typing CRISPR Systems

    Type
    Blog Post
    ... with CRISPR–Cas13. Nature, 550(7675), 280–284. https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan... deep terascale clustering. Science, 382(6673). https://doi.org/10.1126/science.adi1910 Chylinski, K.,...systems. Nucleic Acids Research, 42(10), 6091–6105. https://doi.org/10.1093/nar/gku241 Cong, L., Ran, F. A...CRISPR/CAS systems. Science, 339(6121), 819–823. https://doi.org/10.1126/science.1231143 Cox, D. B. T.,...with CRISPR-Cas13. Science, 358(6366), 1019–1027. https://doi.org/10.1126/science.aaq0180 Harrington, L....CRISPR-Cas14 enzymes. Science, 362(6416), 839–842. https://doi.org/10.1126/science.aav4294 Hu, C., Myers,...applications. Molecular Cell, 84(3), 463-475.e5. https://doi.org/10.1016/j.molcel.2023.12.034 Kolesnik,...

  14. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... Gene Therapy: An Overview. Cells, 12(5), 1–41. https://doi.org/10.3390/cells12050785 Murlidharan, G.,...system. Frontiers in Molecular Neuroscience, 7. https://doi.org/10.3389/fnmol.2014.00076 References and...and 9 in the Mouse Brain. PLOS ONE, 8(9), e76310. https://doi.org/10.1371/journal.pone.0076310 Damdindorj...adeno-associated viral vectors. PLoS ONE, 9(8), 1–10. https://doi.org/10.1371/journal.pone.0106472 de Backer...Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169 Dudek, A. M., Pillay...Independent of AAV Receptor. Journal of Virology, 92(7). https://doi.org/10.1128/jvi.02213-17 Favre, D., Blouin...Virus. Journal of Virology, 76(22), 11605–11611. https://doi.org/10.1128/JVI.76.22.11605-11611.2002 Griffin...

  15. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...miRFP703 https://www.addgene.org/79988/ 674 703 90,900 8.6 127 Monomer 394 4.5 37 miRFP709 https://www.addgene.org...mIFP https://www.addgene.org/54620/ 683 705 82,000 8.4 112 Monomer 54 4.5 15  15 miRFP https://www.addgene.org....0 e https://www.addgene.org/54785/ 688 709 98,000 8.1 128 Dimer 108 4.5 8  17 iRFP670 https://www.addgene.org...iRFP682 https://www.addgene.org/45459/ 663 682 90,000 11.1 162 Dimer 490 4.5 105 iRFP702 https://www.addgene.org...iRFP713 https://www.addgene.org/31857/ 690 713 98,000 6.3 100 Dimer 960 4.5 100  19 iRFP720 https://www.addgene.org...emiRFP703 https://www.addgene.org/136558/ 674 703 90,900 8.6 127 Monomer 700 4.5 90 miRFP713 https://www.addgene.org...iRFP713, % d Ref BphP-based FPs miRFP670 https://www.addgene.org/79987/ 642 670 87,400 14.0 198...

  16. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...Science (PLoS), 18 Feb. 2011, p. e16765. Crossref, https://doi.org/10.1371/journal.pone.0016765. Moore, Simon...Society (ACS), 2 May 2016, pp. 1059–69. Crossref, https://doi.org/10.1021/acssynbio.6b00031. Iverson, Sonya...Society (ACS), 4 Nov. 2015, pp. 99–103. Crossref, https://doi.org/10.1021/acssynbio.5b00124. Obst, Ulrike...Society (ACS), 15 Mar. 2017, pp. 1016–25. Crossref, https://doi.org/10.1021/acssynbio.6b00337. Lee, Michael...Society (ACS), 1 May 2015, pp. 975–86. Crossref, https://doi.org/10.1021/sb500366v. Shaw, William M., et...Society (ACS), 6 Nov. 2023, pp. 3393–405. Crossref, https://doi.org/10.1021/acssynbio.3c00423. Pérez-González...and Business Media LLC, 13 Nov. 2017. Crossref, https://doi.org/10.1186/s12896-017-0393-y. Engler, Carola...

  17. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...and its host response. Nat Methods 13:868–874 . https://doi.org/10.1038/nmeth.3993  Cong L, Ran FA, Cox... Using CRISPR/Cas Systems. Science 339:819–823 .https://doi.org/10.1126/science.1231143  Cox DBT, Gootenberg...editing with CRISPR-Cas13. Science 358:1019–1027 . https://doi.org/10.1126/science.aaq0180  Fine EJ, Appleton...using compact expression cassettes. Sci Rep 5: . https://doi.org/10.1038/srep10777  Garneau JE, Dupuis ...bacteriophage and plasmid DNA. Nature 468:67–71 . https://doi.org/10.1038/nature09523  Ibraheim R, Song ...Neisseria meningitidis Cas9 in vivo. Genome Biol 19: . https://doi.org/10.1186/s13059-018-1515-0  Kim E, Koo ...derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038/ncomms14500  Levy JM, Yeh W-H,...

  18. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...and 9 in the mouse brain. PloS One, 8(9), e76310. https://doi.org/10.1371/journal.pone.0076310 Bish, L. ...and Rat. Human Gene Therapy, 19(12), 1359–1368. https://doi.org/10.1089/hum.2008.123 Dalkara, D., Byrne...Science Translational Medicine, 5(189), 189ra76. https://doi.org/10.1126/scitranslmed.3005708 Goertsen,...Therapy. Methods & Clinical Development, 26, 331–342. https://doi.org/10.1016/j.omtm.2022.07.010 Issa, S. S....in Gene Therapy: An Overview. Cells, 12(5), 785. https://doi.org/10.3390/cells12050785 Kaludov, N., Brown...Specificity. Journal of Virology, 75(15), 6884–6893. https://doi.org/10.1128/jvi.75.15.6884-6893.2001 Pillay...adeno-associated virus infection. Nature, 530(7588), 108–112. https://doi.org/10.1038/nature16465 Rubin, J. D., Nguyen...

  19. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...conservation of a conoid. PLoS Biology 19:e3001020. https://doi.org/10.1371/journal.pbio.3001020  Chen F, ...2015) Expansion microscopy. Science 347:543–548. https://doi.org/10.1126/science.1260088 Damstra HG, Mohar...Robust Expansion Microscopy (TREx). Elife 11:e73775. https://doi.org/10.7554/elife.73775  Dani A, Huang B, ...Chemical Synapses in the Brain. Neuron 68:843–856. https://doi.org/10.1016/j.neuron.2010.11.021  Gambarotto...expansion microscopy (U-ExM). Nat Methods 16:71–74. https://doi.org/10.1038/s41592-018-0238-1  Klimas A, Gallagher... for expansion microscopy. Nat Biotechnol 1–12. https://doi.org/10.1038/s41587-022-01546-1  Mikhaylova...using anti-tubulin nanobodies. Nat Commun 6:7933. https://doi.org/10.1038/ncomms8933  M’Saad O, Bewersdorf...

  20. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ... Caenorhabditis elegans. Genetics 198:837–846 . https://doi.org/10.1534/genetics.114.169730 Bottcher R...Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289 Chen C, Fenk LA, de...recombination. Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani ...Drug Selection Cassette. Genetics 200:1035–1049 . https://doi.org/10.1534/genetics.115.178335 Dokshin GA... Caenorhabditis elegans. Genetics 210:781–787 . https://doi.org/10.1534/genetics.118.301532 Ebbing A, ...alternative gene editing system for AT-rich sequences https://doi.org/10.17912/W2237D Farboud B, Severson AF...Editing Using CRISPR-Cas9. Genetics 211:431–457 . https://doi.org/10.1534/genetics.118.301775 Ghanta KS,...

  21. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...null,"href":"https://www.addgene.org/collections/mammalianrnai/","href_with_scheme":"https://www.addgene.org...D. P. (2004). MicroRNAs. Cell, 116(2), 281–297. https://doi.org/10.1016/s0092-8674(04)00045-5 De Rie, ...and mouse. Nature Biotechnology, 35(9), 872–878. https://doi.org/10.1038/nbt.3947 Fire, A., Xu, S., Montgomery...Caenorhabditis elegans. Nature, 391(6669), 806–811. https://doi.org/10.1038/35888  Iwakawa, H., & Tomari, ...silencing complex. Molecular Cell, 82(1), 30–43. https://doi.org/10.1016/j.molcel.2021.11.026 Kilikevicius...silencing. Nucleic Acids Research, 50(2), 617–634. https://doi.org/10.1093/nar/gkab1256 Lee, Y., Kim, M.,...polymerase II. The EMBO Journal, 23(20), 4051–4060. https://doi.org/10.1038/sj.emboj.7600385 McIntyre, G. ...

  22. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ... editing. Nature Biotechnology, 41(5), 663–672. https://doi.org/10.1038/s41587-022-01532-7 Gaudelli, N...without DNA cleavage. Nature, 551(7681), 464–471. https://doi.org/10.1038/nature24644 Gaudelli, N. M., Lam...application. Nature Biotechnology, 38(7), 892–900. https://doi.org/10.1038/s41587-020-0491-6 Grünewald, J... editing. Nature Biotechnology, 38(7), 861–864. https://doi.org/10.1038/s41587-020-0535-y Kim, Y. B., ... fusions. Nature Biotechnology, 35(4), 371–376. https://doi.org/10.1038/nbt.3803 Koblan, L. W., Doman,...reconstruction. Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt.4172 Komor, A. C., Kim, Y....double-stranded DNA cleavage. Nature, 533(7603), 420–424. https://doi.org/10.1038/nature17946 Komor, A. C., Zhao...

  23. An Introduction to Adenovirus

    Type
    Blog Post
    ...native viral promoters. Mol Ther, 7(4), 526–534. https:/doi.org/10.1016/S1525-0016(03)00023-6. PMID: 12727116...evidence. Emerg Microbes Infect, 8(1), 1679–1687. https://doi.org/10.1080/22221751.2019.1690953. PMID: 31749409...Reference Module in Biomedical Sciences. Elsevier. https://doi.org/10.1016/B978-0-12-801238-3.02523-X. Hofmann...Adenoviruses In Vivo. J Virol, 73(8), 6930–6936. https://doi.org/10.1128/JVI.73.8.6930-6936.1999. PMID:...therapy. Proc Natl Acad Sci U S A, 95(19), 11377. https://doi.org/10.1073/PNAS.95.19.11377. PMID: 9736744...Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/10.1371/journal.ppat.1004821. PMID: 26042599...adenoviruses. J Clin Pathol Suppl (R Coll Pathol)., 6, 39. https://doi.org/10.1136/jcp.s3-6.1.39. PMID: 4376152. ...

  24. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...The unexhausted potential of E. coli. ELife, 4. https://doi.org/10.7554/elife.05826 Blum, M., & Ott, T...Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org/10.1159/000490898 Gibbs, R., Weinstock...mammalian evolution. Nature 428, 493–521 (2004). https://doi.org/10.1038/nature02426 Iannaccone, P. M.,...! Disease Models & Mechanisms, 2(5–6), 206–210. https://doi.org/10.1242/dmm.002733 Idalia, V.-M. N., &...Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef, M., & Meinke,...model plant. The Plant Journal, 61(6), 909–921. https://doi.org/10.1111/j.1365-313x.2009.04086.x Krämer...ecological context with Arabidopsis thaliana. ELife, 4. https://doi.org/10.7554/elife.06100 Liu, L. S., Zhao, ...

  25. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...delivery in mice. J Clin Invest 118:1955–1964 . https://doi.org/10.1172/jci34316 Bak RO, Porteus MH (2017...Using AAV Donor Vectors. Cell Reports 20:750–756 . https://doi.org/10.1016/j.celrep.2017.06.064  Carvalho...Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org/10.3389/fnins.2017.00503  Chamberlain ...Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org/10.1089/hgtb.2015.140 Frischmeyer PA (...Lacking Termination Codons. Science 295:2258–2261 . https://doi.org/10.1126/science.1067338 Ghosh A, Yue Y...Transgene-independent Manner. Molecular Therapy 16:124–130 . https://doi.org/10.1038/sj.mt.6300322 Halbert CL, Allen...parts of a larger gene. Nat Biotechnol 20:697–701 . https://doi.org/10.1038/nbt0702-697 Hirsch ML, Agbandje-McKenna...

  26. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...using the CRISPR/Cas system. Plant Methods 9:39 . https://doi.org/10.1186/1746-4811-9-39 Gao Q, Xu W, Yan...cereal genomic studies. New Phytol 223:2120–2133 . https://doi.org/10.1111/nph.15889 Gelvin SB (2003) Agrobacterium-Mediated...behind the “Gene-Jockeying” Tool. MMBR 67:16–37 . https://doi.org/10.1128/mmbr.67.1.16-37.2003 Khatodia ...Application in Improvement of Crops. Front Plant Sci 7: . https://doi.org/10.3389/fpls.2016.00506 Kwak S-Y, Lew ...nanotube carriers. Nat Nanotechnol 14:447–455 . https://doi.org/10.1038/s41565-019-0375-4 Lowder LG, Zhang...Transcriptional Regulation. Plant Physiol 169:971–985 . https://doi.org/10.1104/pp.15.00636 Ma X, Zhang X, Liu...virally delivered CRISPR–Cas9. Nat Plants 6:773–779 . https://doi.org/10.1038/s41477-020-0704-5 Santana I, Wu...

  27. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...epigenome editing. Nucleic Acids Research 47:e23–e23. https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito...and enhancers. Nature Biotechnology 33:510–517 . https://doi.org/10.1038/nbt.3199 Find plasmids from this...human genome. Nature Biotechnology 35:561–568 . https://doi.org/10.1038/nbt.3853 Find plasmids from this...-MQ1 fusion protein. Nature Communications 8: . https://doi.org/10.1038/ncomms16026 Find plasmids from...in the Mammalian Genome. Cell 167:233–247.e17 . https://doi.org/10.1016/j.cell.2016.08.056 Find plasmids...fusion proteins. Nature Biotechnology 31:1137–1142 . https://doi.org/10.1038/nbt.2726 Find plasmids from this...site-specific DNA methylation. Biology Open 5:866–874 . https://doi.org/10.1242/bio.019067 Find plasmids from...

  28. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...targets. Nature Communications, 7(1), Article 1. https://doi.org/10.1038/ncomms10778 Erdmann, R. S., Baguley...SNAP-tags. Cell Chemical Biology, 26(4), 584-592.e6. https://doi.org/10.1016/j.chembiol.2019.01.003 Gallagher...Reactivity. ACS Chemical Biology, 4(7), 547–556. https://doi.org/10.1021/cb900062k Gautier, A., Juillerat...Living Cells. Chemistry & Biology, 15(2), 128–136. https://doi.org/10.1016/j.chembiol.2008.01.007 Griffin...Inside Live Cells. Science, 281(5374), 269–272. https://doi.org/10.1126/science.281.5374.269 Jing, C.,... Accounts of Chemical Research, 44(9), 784–792. https://doi.org/10.1021/ar200099f Keppler, A., Gendreizig...in vivo. Nature Biotechnology, 21(1), Article 1. https://doi.org/10.1038/nbt765 Lavis, L. (2016, September...

  29. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...double-strand breaks or donor DNA. Nature 576:149–157 . https://doi.org/10.1038/s41586-019-1711-4 Avery OT, MacLeod...The Journal of Experimental Medicine 79:137–158 . https://doi.org/10.1084/jem.79.2.137 Carroll D (2011) ...With Zinc-Finger Nucleases. Genetics 188:773–782 . https://doi.org/10.1534/genetics.111.131433 Doudna JA,...engineering with CRISPR-Cas9. Science 346:1258096 . https://doi.org/10.1126/science.1258096 Gaudelli NM, Komor...genomic DNA without DNA cleavage. Nature 559:E8–E8 . https://doi.org/10.1038/s41586-018-0070-x Griffith F (...Pneumococcal Types. Journal of Hygiene 27:113–159 . https://doi.org/10.1017/s0022172400031879 Hershey AD, ...BACTERIOPHAGE. The Journal of General Physiology 36:39–56 . https://doi.org/10.1085/jgp.36.1.39 Komor AC, Kim YB, ...

  30. To Codon Optimize or Not: That is the Question

    Type
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