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We narrowed to 400 results for: pho;

Showing: 1 - 30 of 400 results

  1. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...generate the phosphosites with serine instead of phosphoserine (i.e. a “phosphorylatable” phosphosite library... serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library ). The phosphorylation sites (“...Reagents Rinehart Lab Phosphoprotein Reagents Protein phosphorylation is one of the most abundant forms of...questions surrounding serine phosphorylation by adding this phosphorylated amino acid to the genetic code...or multiply phosphorylated proteins (Pirman et al., Nat Comm 2015). Improved phosphoprotein production ...(“phosphosites”) consist of a central phosphoserine site flanked by 15 amino acids on either side occurring... site-specific incorporation of phosphoserine in >36,000 phosphosites by tandem mass spectrometry (Barber...

  2. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...SDS-PAGE gels containing Phos-Tag, a small molecule that binds specifically to phosphate moieties and promotes... al. "Detection of phosphorylation on large proteins by Western blotting using Phos-tag containing gel...MEK1 phosphomimetic residues in the above study with phosphoserine (to create a pSer/phosphomimetic dual...shift in phosphorylated, but not non-phosphorylated proteins (8). Quantifying the ratio of phospho- and non-phospho-bands...of the dephosphorylation reaction can be tracked through the comparison of phospho:non-phospho species...modified through phosphorylation (1). However, the expansion of studies on protein phosphorylation in an array...suggest that phosphorylation is far more prevalent than previously appreciated. PhosphoSitePlus, one of the...

  3. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...or dark conditions, the chromophore activates the RR (OmpR) via phosphorylation, promoting transcription...histidine kinase/photosensor senses various light inputs, and an obligate chromophore allows the system...dark conditions, the chromophore activates the response regulator via phosphorylation, promoting transcription...in E. coli easier and more robust. Synthetic photobiology: why use light? In synthetic biology and bioengineering...TCSs). TCSs consist of a histidine kinase that phosphorylates a response regulator (RR). TCS-induced responses...two-component system (TCS). Levskaya et al. fused a photosensory domain from cyanobacteria to a common E. coli...hybrid construct senses light, and an obligate chromophore allows the system to respond to various light...

  4. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...state contains a fluorophore and a quencher in close proximity. For instance, the fluorophore and quencher...of aptamers means they can be used with or as fluorophores in several different ways. Researchers have ...fluorescent in situ hybridization (FISH) where fluorophore-linked antibodies are used to visualize RNA. ...removing the quencher from the proximity of the fluorophore and subsequently leading to a positive signal...signal. The same effect can be achieved for a fluorophore that relies on dimerization for activation, only...used as powerful biosensors, the bound protein fluorophores and quenchers are still relatively bulky. One...fluor. Transcribing and translating a protein fluorophore also takes time - bypassing translation altogether...

  5. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...dimerizing photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation) domains on... Figure 1: Photoswitchable MEK1 (psMEK1). Is it possible to create other photoswitchable kinases? The...Signal transduction pathways are a lot like cell phone networks. Protein kinases deliver messages to the...-specifically activated and, like the game of telephone, often the signal must be transmitted by intermediate... lab present a generic design for creating photoswitchable kinases and use MEK1 as a model kinase. In ...site of a constitutively active MEK1. This photoswitchable MEK1 is called psMEK1. When exposed to 500 ...psMEK1 also worked for creating three other photoswitchable kinases: psMEK2, psRaf1, and psCDK5. MEK2 is...

  6. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...biological research. Photoconvertible fluorescent proteins Newly developed photoconvertible FPs like PsMOrange...Applications and limitations of GFP photoconversion One concern with photoconverting GFP is the expression level...and efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible...Davidson MW, Looger LL. A bright and photostable photoconvertible fluorescent protein. Nat Methods. 2009...accidental discovery that laser treatment can photoconvert GFP from green to RED! This simple technique...time-consuming to repeat the process with these new photoconvertible proteins. With Sattarzadeh et al.’s discovery...discovery that GFP itself is photoconvertible, new studies of spatiotemporal dynamics can be undertaken using...

  7. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...emitted photons before they are destroyed. Factors that affect photostability The photobleaching rate of...will discuss how to select a photostable fluorescent protein. Photobleaching is the irreversible destruction...destruction of a fluorophore under the influence of light. Any fluorescent molecule will photobleach at some point...have fluorescent proteins that are photostable. On top of photobleaching, fluorescent proteins may display...likelihood that a fluorophore will bleach. This is, however, not the full story. The photobleaching rate does ... 2-fold does not reduce photobleaching by half. How exactly the photobleaching rate depends on power is...to compare the photostability of different fluorescent proteins? Measuring photostability Since it is clear...

  8. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...illuminated with UV-violet light (~400 nm), the chromophore photoconverts predominantly to the anionic form, causing...presented first, it causes irreversible photoconversion of the chromophore from the neutral to the anionic form...mEosFP, can form it’s chromophore at a more comfortable 37°C (5). Dendra2 photoconverts from green to red ...reversible PA-FPs (also known as photoswitchers) can be photoswitched from a dark state to a bright fluorescent... for vanishing, and pa, for photoactivation) (6). Dronpa is photoactivated by blue wavelengths of light...consider, such as brightness, photostability, pH stability, chromophore maturation rate at 37 °C, turnover...-FPs offer a reduced potential for photobleaching and phototoxicity that standard FPs might induce though...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...biophysical interaction between two fluorophores. When the first fluorophore (the donor) is excited, it can...acids/lipids of interest are fluorophore labeled and that these fluorophores can undergo FRET when they ...targeting a FRET fluorophore to a biomolecule is a bit like a Fedex delivery. Your fluorophore package needs... energy directly via resonance to the second fluorophore (the acceptor) if the two probes are close together...physical distance between the donor/acceptor fluorophores (Stryer, 1978), a measurement that is unaffected...label your biomolecule of interest with FRET fluorophores? When is absolute labeling specificity needed...measure it). Molecular mailbox: How to get your fluorophore delivered to the right place? FRET experiments...

  10. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...technique! What is a photosensitizer? First, for a few definitions. A photosensitizer is a dye or protein... higher photostability than KillerRed, with KillerRed reaching greater than 75% photobleaching after just...system? Turns out that with light-inducible photosensitizers and a quick zap of the proper color light,...light, you can do just that.  Photosensitizing dyes and proteins have been around for awhile (check out this...Bruchez and Tsang labs recently developed a photosensitizer composed of the protein complex complex and... oxygen species (ROS). The ROS produced by photosensitizers inactivate target proteins or cells while ...having little effect on neighboring cells. The photosensitizer used in this work is composed of two parts:...

  11. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...called “the photon budget”: each sample has a limited number of fluorophores and each fluorophore can emit...the photon budget and pushing the frontiers of fluorescence microscopy often requires more photons. For...cells can decrease the number of fluorophores, compromising the photon budget. Likewise, the switch from...desperate to extract a few more photons from a sample. Chemical fluorophores can be substantially brighter...more photostable than fluorescent proteins, providing a straightforward way to improve the photon budget...rhodamine fluorophores, such as the Alexa Fluor and ATTO dyes, with improved brightness, photostability, and... of photons for your imaging experiment. Innovative labeling strategies and improved fluorophores are ...

  12. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...Listen to the photoswitchable protein podcast segment Reversible control of photoswitchable proteins, which...., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain from A. sativa into a loop...ratio, high sensitivity, responds quickly, is photostable, and is compatible with optogenetic control. ... Discovery of new protein binder tools for photoswitchable proteins cPYP and AsLOV2 Article contributed...temporally control cellular processes in vivo. Many photoactive proteins exist in nature, but developing them... either the light- and dark- states of the photoswitchable cPYP, which changes in conformation in response...bind to only the light- or dark- state of the photoswitchable proteins offers a powerful approach to control...

  13. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PLCG2 phospholipase C, gamma 2 (phosphatidylinositol-specific) - PPP3CA protein phosphatase 3 (formerly...IP1, IP2, IPD2, NEMO INPP5D inositol polyphosphate-5-phosphatase, 145kDa MGC104855, MGC142140, MGC142142...FLJ26625, JTK8 MALT1 mucosa associated lymphoid tissue lymphoma translocation gene 1 DKFZp434L132, MLT... PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase...and cytoplasmic domain, (semaphorin) 6D FLJ11598, KIAA1479 SEMA7A semaphorin 7A, GPI membrane anchor (...BMP1 bone morphogenetic protein 1 FLJ44432, PCOLC, PCP, TLD, pCP-2 BMP10 bone morphogenetic protein 10...MGC126783 BMP15 bone morphogenetic protein 15 GDF9B, ODG2, POF4 BMP2 bone morphogenetic protein 2 BMP2A BMP3...

  14. Genetic Code Expansion

    Type
    Collection
    ... Expansion Accessible Using Phosphoserine to Study Protein Phosphorylation Rinehart Lab reagents tRNA/... mutated, serB deleted for phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic... pMAH2-CageCys leucyl-tRNA-synthetase E. coli photocaged cysteine Mammalian TAG Huiwang Ai 73544 pEvol-pAcFRS...91705 pSupAR-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Bacterial TAG P....91706 pCMV-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Mammalian TAG P.... PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser...140022 pAS_4xMma PylT_FLAG-PcKRS PcKRS M. barkeri photocaged-lysine Mammalian TAG Simon Elsaesser 140023 pAS...

  15. Antibody Guide

    Type
    Collection
    ... curve with known amounts of protein. Fluorophores Fluorophores are light-sensitive molecules which emit...IF uses fluorophore color to differentiate between the targets of interest, if your fluorophore is bound...the fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with...If a bright fluorophore is on an abundant target, it obscures fainter signals. Fluorophores can emit signals...storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °C. Certain isotype...molecules are horseradish peroxidase (HRP) and fluorophores. Horseradish peroxidase (HRP) HRP performs a...specific wavelength. While more expensive than HRP, fluorophores are available in a range of colors activated...

  16. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Webb Phosphate FRET-based phosphate sensor (FLIPPi) A novel analytical method for in vivo phosphate tracking...Ca(2+) indicator with enhanced two-photon absorption. Neurophotonics. 2024 Apr;11(2):024207. doi: 10.1117...29;7. pii: 32638. Kai Johnsson Phosphate Scaffold for inorganic phosphate biosensor Development of a Reagentless.... Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based calcium integrator...calcium sensor with exceptional brightness, photostability, and multiplexing capabilities. bioRxiv. 2025... jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths. Nat Methods...localized zinc sensor ZIBG2 Genetically Encoded, Photostable Indicators to Image Dynamic Zn(2+) Secretion ...

  17. Ras Pathway

    Type
    Collection
    ... Protein phosphatase 1 catalytic subunit alpha PREX2 Phosphatidylinositol-3,4,5-trisphosphate-dependent...receptor PDPK1 3-phosphoinositide dependent protein kinase 1 PEBP1 Phosphatidylethanolamine binding protein...PIK3R5 PIK3R6 Also known as PI3K; Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory...state, regulated by the addition and removal of a phosphate group. Under normal physiological conditions GTPases...thymoma viral oncogene homolog 3 ALK Anaplastic lymphoma receptor tyrosine kinase ARHGAP35 Rho GTPase activating...DUSP2 DUSP3 DUSP4 DUSP5 DUSP6 Dual specificity phosphatase E2F E2F1 E2F2 E2F3 E2F transcription factor ECT2...G1, G2, G3: non-catalytic subunit gamma PTEN Phosphatase and tensin homolog RAC RAC1 RAC2 RAC3 Ras-related...

  18. Optogenetics AAV Preps

    Type
    Collection
    ... Dlx EF1a/nEF Synapsin E2 regulatory element Fluorophore GFP Red-wavelength fluorescent protein Yellow-wavelength...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent 1, 5 Deisseroth... 6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive 8 Deisseroth... pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-nEF-Con/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Cre and Flp dependent...

  19. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... multicolor imaging. Photo-activatable iRFPs can be ‘turned on’ by non-phototoxic far-red light and are...690/717 (after photoactivation) 3.2 pPAiRFP1-N1 PAiRFP2 692/719 (after photoactivation) 3.0 pPAiRFP2-N1... imaging using far red, near-infrared, and photoactivatable fluorescent proteins. Plasmid...imaging are derived from bacterial phytochrome photoreceptors (BphPs). Spectrally distinct permanently fluorescent...The following far red , near-infrared , and photoactivatable fluorescent empty backbone plasmids are ideal...piRFP720-N1 iSplit 690/713 5.3 pPAS-E and pK-GAFm Photoactivatable Protein Excitation/Emission Brightness Find...

  20. Bacterial Expression Systems

    Type
    Collection
    ... Large Stokes Shift , Photoactivatable , Photoconvertible , and Photoswitchable fluorescent proteins Various...monitor in live cells (beware of artifacts!), photoactivatable tags for super resolution imaging, epitope...20341 mEos2 (fluorescent protein) Loren Looger Photoactivable Fluorescent Protein Expression: contains mEos2...mEos2 with an N-terminal His tag. Photoactivable fluorescent proteins like this one can be used in super...protein, MS2. Can be used in combination with a fluorophore-tagged MS2 (such as that found in pZS*12-MS2-... to dsRED. Can be used in combination with a fluorophore-tagged MS2 (such as that found in pZS*12-MS2-...enhances the FRET signal from the CFP and YFP fluorophores linked by an arabinose binding protein (AraF...

  21. Luciferase Plasmid Collection

    Type
    Collection
    ... of photons. The robust signal, sensitivity, wide dynamic range, and resistance to photobleaching has ... tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Conformationally... sensor created by fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Nano-lanterns...-Leu linker to the circularly permuted Venus fluorophore. Learn more about pHlash sensor in our 28 Hot...Firefly Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash 128046 pGL3basic luciferase and...Insertion of 5' promoter/enchancer regions in Drosopholia Herman Wijnen 126033 pTETRIS-cargo Firefly For...

  22. Cre-lox system

    Type
    Collection
    ...pAAV-CIBN-CreC photo-activatable cre recombinase CAG AAV Bruchas 75268 pAAV-CRY2-CreN photo-activatable ... Cre and gRNAs cTnT AAV Pu 87694 pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2 light-activated Cre cTnT Mammalian Campbell...Cepko 13780 pNrl-Cre Cre-Myc, Expressed in rod photorecetor cells in the retina Nrl Mammalian Cepko 13962...pAAV-Syn-iCre iCre AAV Ritola 122960 pcDNA3.1_PA-Cre photo-activatable Cre recombinase CMV Mammalian Sato 122961...pBbE5a-Opto-Cre-Vvd Cre recombinase split with Vivid photodimers lacUV5 Bacterial Dunlop 135217 pDEST mfap4:icre-p2a-tomato...pAAV-EF1a-NCreV N-terminal Cre component split with Vivid photodimers EF-1 alpha AAV Zeng 140132 pAAV-EF1a-CCreV C-...C-terminal Cre component split with Vivid photodimers EF-1 alpha AAV Zeng 140135 pAAV-EF1a-iCreV Split...

  23. Recombinases AAV Preps

    Type
    Collection
    ...these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI....AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA...Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA...Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none...none 1, PHPeB Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre EF1a ...

  24. Zhang Lab CRISPR Page

    Type
    Collection
    ...to enable the rapid ligation of annealed and phosphorylated oligos designed based on the target site sequence...

  25. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Near-Infrared Long Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Blue/UV Protein Excitation...LSSmKate2-C1 - Mammalian Expression Jump to Top Photoactivatable (e.g. off to on) Excitation and Emission wavelengths...HisB-PAiRFP2 - Bacterial Expression Jump to Top Photoconvertible (e.g. green to red) Protein Excitation (nm...pBAD-PSmOrange2 - Bacterial Expression Jump to Top Photoswitchable (e.g. off to on to off) Protein Excitation ...

  26. mTOR Pathway

    Type
    Collection
    ...PIK3R2 PIK3R3 PIK3R4 PIK3R5 PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory...AKT1S1; AKT1 substrate 1 (proline rich) PTEN Phosphatase and tensin homolog Rag RRAGA RRAGB RRAGC RRAGD...Protor Also known as PRR5; proline rich 5 PTEN Phosphatase and tensin homolog Rictor RPTOR independent companion...

  27. p53 Pathway

    Type
    Collection
    ...deprivation. p53 is activated through both phosphorylation and acetylation, which disrupt its interaction...ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-13-acetate-induced protein 1; also... 1 (RCHY1); E3 ubiquitin protein ligase PTEN Phosphatase and tensin homolog PUMA BCL2 binding component...

  28. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...2.1 may be omitted. CRISPR Cloning Anneal and phosphorylate oligos. Resuspend oligos at a concentration ...Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated oligos may be ordered instead. For this approach...

  29. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...followed by a quick ligation of annealed and phosphorylated oligonucleotides containing the guide target...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...

  30. Antibody Production

    Type
    Collection
    ...are initially titered by spectrophotometry using a NanoDrop spectrophotometer. If needed, the sample is...centrifugal columns. The final formulation buffer is phosphate-buffered saline containing 1 mM sodium azide. ...
Showing: 1 - 30 of 400 results