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We narrowed to 232 results for: SPL;

Showing: 1 - 50 of 232 results

  2. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...Cui Red mScarlet Split-wrmScarlet variant of mScarlet Split-wrmScarlet and split-sfGFP: tools for faster...chromophore maturation, splitFAST displays rapid and reversible complementation. TagBiFC (split HaloTag) provides...Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein tools: Split-TurboID... new and popular split-FP plasmids from our catalog to help you find the right split-FP plasmid for your...cells with split fluorescent protein. Kamiyama et al. Nat Commun. 2016 Bo Huang spGFP Split superpositive...(Alice Ting Lab 2020) Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome...to fuse a protein of interest to each part of a split fluorescent protein (FP) and measure the signal ...

  3. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...outside the native cell context. The Split-BioID method Enter Split-BioID, a spatiotemporally controllable...within a ~10 nm radius of the bait. In Split-BioID, BirA* is first split into two non-functional fragments....labeled. Figure 1: Using Split-BioID to Identify Protein Protein Interactions. Split-BioID is a protein complementation...controls using Split-BioID-GFP and BioID run on six unrelated proteins. One caveat of Split-BioID is that...localization of the fusion protein. Advantages of Split-BioID Split-BioID’s key advantage over other PPI methods...Important considerations for Split-BioID It’s important to remember that with Split-BioID, and BioID, any protein...version of Split-BioID. BirA* is fragmented at a different site than in Schopp et al. Find these Split-BioID...

  4. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...EcAR7 Plasmid 112031 pNAS1b split mCherry 14-3-3β ctrl Plasmid 112030 pNAS1b split mCherry NEDD4 WW2 neg ctrl...Plasmid 112029 pNAS1b split mCherry NEDD4 WW2 pos ctrl Plasmid 111883 pNAS1b split mCherry NEDD4-2 WW2 ...WW2 Plasmid 111882 pNAS1b split mCherry NEDD4 WW2 Plasmid 111881 pNAS1b split mCherry 14-3-3σ Plasmid 111880...introduced into a second expression vector encoding a split mCherry fluorescent reporter, enabling the detection...phosphosite library is fused to the N-terminal portion of split mCherry, while a phosphobinding domain is fused ...111880 pNAS1b split mCherry 14-3-3β Pooled Library 111704 Mode #1 Library Pooled Library 111705 Mode #2...

  5. Cre-lox system

    Type
    Collection
    ...VloxP and SloxP sites respectively. Split Cre: Cre recombinase is split in half into N and C terminal Cre...pCMV-Tag2B-NCre N-terminal split-Cre CMV Mammalian Hirrlinger 106369 pCMV-Tag3B-CCre C-terminal split-Cre CMV Mammalian...N-terminal split-Cre-ERT2 CMV Mammalian Hirrlinger 106371 pCMV-Tag3B-ERT2CCre C-terminal split-Cre-ERT2 ...Cre component split with Vivid photodimers EF-1 alpha AAV Zeng 140135 pAAV-EF1a-iCreV Split Cre fused to...expression ApoE.HCR.hAAT Mammalian Ehmer 85797 pSkipFlox split Cre Pfcrt P. falxiparum Spielmann 86641 pLenti-hSynapsin-Cre-WPRE...Nedivi 134404 pBbE5a-Opto-Cre-Vvd Cre recombinase split with Vivid photodimers lacUV5 Bacterial Dunlop 135217...140131 pAAV-EF1a-NCreV N-terminal Cre component split with Vivid photodimers EF-1 alpha AAV Zeng 140132...

  6. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...the AAV2 serotype. The AAV2(Y444F) serotype also displays enhanced transduction of the trabecular meshwork...Y730F. AAV2(4pMut)dHS The AAV2(4pMut)dHS serotype displays enhanced transduction of the retina following .... AAV6(dbY-F+T-V) The AAV6(dbY-F+T-V) serotype displays enhanced transduction of hematopoietic stem cells... Y731F and T492V. AAV44.9 The AAV44.9 serotype displays highly efficient transduction and lateral spread...Boye, et al. 2020. Novel AAV44.9-Based Vectors Display Exceptional Characteristics for Retinal Gene Therapy...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...approaches. Split-Luciferase and Protein-Protein Interaction Assays Cluc-FKBP , FRB-Nluc : Split Firefly ...complementation : Protein-protein assays based on a split NanoLuc® have been used to assess GPCR-G protein...-penetrating peptides , and more. nLuc/cLuc for split Firefly Luciferase complementation in plants. Bioluminescence...Biology CMV-LUC2CP/intron/ARE, CMV-LUC2CP/ARE : A splicing reporter that contains an intron in the middle...Creation of N-terminal Firefly luciferase fragment for split-luciferase complementation assay in plants using... of C-terminal Fifrefly luciferase fragment for split-luciferase complementation assay in plants using...

  8. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ... brief overviews of three split vector strategies plus one additional split vector strategy for increasing... homology directed repairs (HDR). Split AAV vector approaches Split vector approaches increase the size...overlapping split vector had an improvement in muscle physiological performance.  2. Trans-splicing Overview...followed by a splice site donor. The AAV encoding part B of the transgene contains a splice site acceptor...optimization of the exon-splitting site sequence can enhance the rate of trans-splicing (Lai et al., 2005)....either the overlapping or the trans-splicing mechanism. The splice site donor and acceptor sequences and...sequence, upstream of the 5’ splice donor and downstream of the 3’ splice acceptor of the transgene to...

  9. Antibody Plasmid Collection

    Type
    Collection
    ... 2018;1827:313-334. Daniel Christ pComb3 phage display vector system for producing human antibodies Synthetic...1995). Carlos Barbas Variant of the pComb3 phage display vector system for isolation of scFv, diabody and...chicken monoclonal antibody fragments by phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81...

  10. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...One way to reconstitute a split protein like Cas9 is to use split inteins. Split inteins are a pair of naturally...challenge that the field has overcome with split AAVs. In general split AAVs break a large transgene in two ..., mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine et al developed...Staphylococcus aureus Cas9 (SaCas9, 1053 amino acids) displayed cleavage activity in mammalian cells. SaCas9 produced...sensitive method, they found that SaCas9 did not display higher levels of off-target activity than SpCas9...length, allowing them to be packaged in AAV.   Split AAV approaches  While finding smaller Cas9 orthologs...developed a proof-of-concept split intein SpCas9 which had modest editing rates in HEK-293T cells when compared...

  11. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ... Magnet proteins from N. crassa Split Cas9, Split Cas9 nickase, Split dCas9 Genome editing by NHEJ and... optogenetic technique can be employed in other split systems where light is required to reconstitute ...Photoactivatable genome modifications by NHEJ and HDR using split systems Later in 2015, the Sato lab unveiled a photoactivatable...). This system is unique in that it utilizes a “split” nuclease - the authors fragmented Cas9 into N-terminal... halves, rendering the Cas9 non-functional when split but regaining functionality when the halves are ... possible to swap out the Cas9 domains in their split fusions and generate a photoactivatable nickase ...Since these initial studies published in 2015, more split systems have been developed for use with CRISPR-...

  12. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...fused to a fast-splicing split intein and co-infected into the cell. Intein splitting removes any exogenous...native peptide bond at the split site. Figure 1: The base editor is split into two plasmids and co-transfected...the therapeutic potential for the split base editors. Find the split base editors at Addgene. Levy et ...deposit please sign up here.        AAV delivery of split base editors Article contributed by Jennifer Tsang...overcome this problem, David Liu’s lab developed split cytosine and adenine base editors that can be reconstituted...two viruses would lower editing efficiency, the split editors transduced at levels similar to that of ...optogenetics. Cre activity is controlled by red light by splitting Cre and fusing each half with PhyB or PIF6, phytochrome-like...

  13. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...to be screened for with in vitro display methods such as phage display (Bradbury et al., 2011). In vitro...hybridomas, but phage display is a popular way to generate scFvs.  For phage display, a pooled library of... Figure 2: Phage display starts with a pooled library of scFvs that are displayed on the coats of bacteriophages... of scFvs is displayed on the coats of bacteriophages, a process which links genotype and phenotype. Incubating... Incubating the phage display library with plate-bound antigen helps select for high affinity scFvs. Bound.... Created with Biorender.com.   Some phage display libraries screen antibody genes from B cells of... entirely synthetic VL and VH sequences. Phage display also allows for screening scFv generated from the...

  14. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...constitutive splicing studies and has been optimized for high efficiency splicing. If intron splicing does not..., called CRISPR-Display, that may help answer this difficult question. CRISPR-Display uses the dCas9 enzyme... and maybe we can write a blog post about it! Split Cas9 system The Cas9 protein is composed of an n-terminal...bi-lobed architecture of Cas9 to engineer a series of "split" cas9 molecules that cannot function in isolation...form a fully functional Cas9 upon dimerization. Splitting wild-type Cas9 into n-terminal (Cas9(N)-2xNLS)...molecule can cleave the target DNA. The inducible split cas9 approach can also be used for activation of...recently used computational protein design, phage display and high throughput binding assays, to engineer...

  15. Hot Plasmids - October 2020

    Type
    Blog Post
    ...into C. elegans? By using a split FP approach. Split-wrmScarlet is a new split red FP that is three times...colleagues at Calico Life Sciences. Like many split FPs, split-wrmScarlet is composed of two FP fragments...with Fibronectin intrabodies generated with mRNA display (FingRs), Xue Han’s lab has developed AAV vectors...2020. https://doi.org/10.1016/j.isci.2020.101330 Split-wrmScarlet, a fluorescent proteins system for Caenorhabditis...times brighter in worms than split-sfCherry3. It was created by Maria Ingaramo, Jerome Goudeau, Cynthia ...products have distinct localizations. Figure 2: Split-wrmScarlet labeling proteins with different subcellular...

  16. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing. A cis-splicing vector ...delivery using reconstitution via mRNA trans-splicing. By splitting a large gene into smaller fragments, delivering... vector 2; BD, binding domain; SDS, splice donor site; SAS, splice acceptor site; pA, polyadenylation ...delivering them by individual AAVs, and then splicing their mRNAs, the functional gene product is reconstituted...here! Riedmayr, L.M., et al. (2023). mRNA trans-splicing dual AAV vectors for (epi)genome editing and gene...

  17. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...Ribosome display The Seeger lab starts with a “pre-enrichment” selection step using ribosome display because...size (up to 1012 members) in comparison to other display systems using phage and yeast (109-1010 members...ribosome, and mRNA. During the screening process, displayed sybodies that bind to the biotinylated target ...can be recovered for the subsequent steps. Phage display mRNA from the enriched pool of sybody candidates...produce a phage library consisting of phages that display the candidate sybodies in their coat protein to...protocol describes going through two rounds of phage display to increase the enrichment of target-specific sybodies...ELISA) The enriched sybody pools from the phage display screens are subcloned (using FX cloning) into the...

  18. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...cleavage frequency. Truncated gRNAs: Truncated gRNAs display less off-target activity. High fidelity Cas9s: ...Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very low off-target activity due to rationally ...without creating a double-strand break. This method displays higher efficiency than HDR-mediated editing. The...

  19. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...“I’ll be there in a few minutes, I just need to split some cells.” Inevitably, this task would take longer...prioritizing. In my anecdote above, I prioritized splitting cells over meeting up with friends, but didn’t...terms of the utility of your tasks. In my cell splitting anecdote, I probably could have grabbed some food... food with friends and come back to the lab to split my cells later. Eating with friends has a somewhat...all the utility (fun) of eating with friends by splitting my cells first. Time management through planning... I probably wouldn’t have fallen into my cell splitting issue at all if I’d planned appropriately. Back...tissue culture hood may have prevented me from splitting cells until late in the day anway. There are a...

  20. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...miniturbo Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-BioID... assemble when in close proximity. Split-BioID consists of a split BirA* molecule that can reassemble ...circumvent these issues, the Ting lab utilized yeast display-based directed evolution to generate two mutants...complex of interest. For more information, read the Split-BioID blog post.  RaPID: RNA-protein interaction...(LPETG). In EXCELL, the mgSrtA variant can be displayed on the surface of a cell of interest, where it...PMCID: PMC3916822. Schopp, Isabel Myriam, et al. "Split-BioID a conditional proteomics approach to monitor...

  21. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...NEBridge SplitSet If you want to design an overhang set specific to your DNA sequence, SplitSet is the .... Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments. ...minimum number of fragments, or defined split regions. SplitSet will then return the highest-fidelity overhang...Fidelity Viewer™; NEBridge GetSet™; and NEBridge SplitSet™. With these tools, NEB successfully performed...

  22. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...on this tool by splitting cGAL4 in two and binding each half to a gp41-1-N-intein. Split inteins are protein...PMC5693259.  Wang, Han, et al. "Split cGAL, an intersectional strategy using a split intein for refined spatiotemporal...knock-down proteins of interest.  Plasmids for cGAL and split cGAL systems The Sternberg lab in 2016 created cGAL...peptides together (Wang et al., 2018).” With the split system, only cells that have both halves of cGAL...

  23. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...score, and type of article. Now, search results display a bar graph of the number of papers with your search...papers with your key term published each year is displayed with your search results and can be used to filter...other manuscripts that heavily cite the paper are displayed. You can also limit searches to particular authors...entry, the number of citations that paper has is displayed at the top of the page, as well as the number ...background, methods, or results section is also displayed. Saving papers Papers can be saved to a Semantic...shared online, or its altmetric score, is also displayed in your feed. By clicking on a paper in your feed...

  24. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...contaminants. Set up a routine to split cells Don’t be lazy about splitting cells, instead try to form a routine..., for example, split cells on a Monday diluting them 1:10, you should be able to split on the following...Thursday, or, at the latest on Friday. It’s best to split cells before the plates are more than 80-85% confluent...metabolism that is toxic to the cells), and need to be split very soon. There is power in numbers! When starting...

  25. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...reporters altogether; In the split-luciferase system, intein-mediated splicing reconstitutes functional luciferase... luciferase protein interact and enable intein splicing (5). Screening with yeast two hybrid systems While... this requirement for transmembrane proteins, a split ubiquitin system has been devised (8). References...Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical probe for detecting protein-protein...interactions in mammalian cells based on protein splicing. Anal Chem. 2001; 73:2516-2521. Pubmed PMID: 11403293...

  26. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...development of the original iRFP has resulted in a split fluorescence complementation probe used for detecting...690/713 6.2 piRFP iRFP720 702/720 5.8 piRFP720-N1 iSplit 690/713 5.3 pPAS-E and pK-GAFm Photoactivatable...

  27. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...reporter, ZipGFP, which utilized the self-assembling split GFP (GFP1-10 and GFP11) to increase fluorescence... this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11) by flipping a single β-strand...11 β-strands and one central ɑ-helix that can be split into 3 parts: (1) β-strands 1-9 (β1-9) and the one...superfolder Cherry (sfCherry). Like FlipGFP, sfCherry was split into  β1-9 and β10-11, where β10-11 was forced into...-protein interaction sensor based on tripartite split-GFP association." Scientific reports 3 (2013): 2854...

  28. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...excited to announce that our plasmid and sequence displays are now powered by GSL Biotech's SnapGene Server...feature library, our updated plasmid and sequence displays are now much easier to interpret and analyze at...CRISPR gRNA cloning. With this more functional display, you can start thinking about your next cloning...experiment early on. Beyond these simple but powerful display improvements, when you click on any plasmid map...enhancements that will further improve the map displays and functions. ...

  29. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... some you’ve likely never heard of. Each entry displays the following information for a Cas enzyme: ...properties Links to relevant resources The homepage displays a graphic describing CasIDs and relevant properties...feature. Activity features: CasID CasPEDIA will display both the cis and trans activity of each entry. ...protospacer-flanking sequence (PFS) in some cases. The resource displays these requirements in regard to their sequence... covered!     Fig. 1 – CasPEDIA Cas ID display information for a given Cas (SpyCas9a) from CasPEDIA...

  30. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...scientists can mutate the transcription start site, splice sites, exons, and/or promoters to knock out lncRNA... and Bester 2021; Zibitt et al. 2021). Mutating splice sites is particularly effective as it can result...Transcription start site (TSS) deletion. (B) Mutations of splice sites. (C) Removal of an exon or a large genomic...noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol. Published online November...expression by lncRNA promoters, transcription, and splicing. Nature. 2016;539(7629):452. doi:10.1038/nature20149...

  32. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    .... This style displays a poster vertically rather than horizontally. Instead of displaying your information...employing a large central box, often used for displaying all of one’s figures, or at least the majority...inserted. The advantage of this style is that it will display your data most prominently, making it the star ...information as columns, you may choose to display it as rows. It can be more catchy because it is generally...

  33. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...range of PAMs like NG, GAA, and GAT, but also displaying increased editing specificity. We’re excited ...recognizing NG, NNG, GAT, and CAA PAMs, while xCas9 3.6 displayed the second best PAM range. Characterizing xCas9... SpCas9 in mammalian cells, finding xCas9 3.7 displayed about the same editing rate as SpCas9 at an NGG...SpCas9. For some non-promiscuous gRNAs, xCas9 3.7 displayed more than a 100 fold decrease in off-target editing...ratio. Importantly, the observed off-target loci displayed a broad range of PAM sequences, indicating they...

  34. AAV for Neuronal Tracing

    Type
    Collection
    ...Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant...rabies virus AAV1 Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement...

  35. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...detecting protein-protein interactions that uses split fluorescent proteins tethered to potential protein...visualize PPIs. This fluorescent protein (FP) is split into two fragments that individually lack fluorescence...fluorescence microscopy. Figure 1: How BiFC works. A split FP (e.g., yellow fluorescent protein, YFP) is reunited...information about the structures of the tested PIPs. Many split FPs have been validated for BiFC (Kodama and Hu,... Hu, 2012). Such controls are necessary because split FPs can associate, albeit at relatively low frequencies...

  36. p53 Pathway

    Type
    Collection
    ...PubMed PMID: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective.... PubMed PMID: 12619118 . p53 mutant mice that display early ageing-associated phenotypes. Tyner SD, Venkatachalam...

  37. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...anchoring domains The original iGluSnFR in the pMinDisplay backbone is fused to a specific peptide segment...TMD) that anchors the sensor to the membrane and displays it on the extracellular side. In addition to the... 4 Sequence schematics for PDGFR, GPI, and SGZ display constructs. Image adapted from Aggarwal et al… ...transmembrane domain in the mammalian expression pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol...

  38. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...array targeting the MAPT exon 10 splice acceptor and two putative splice enhancers. After AAV-mediated delivery...previously characterized Cas13 enzymes, CasRx also displays very high on-target efficiency; where shRNA treatment...produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9, for which efficiency varies...

  39. CRISPR Guide

    Type
    Collection
    ... reverse transcription. The edited DNA strand displaces the unedited 5’ flap, and the resulting heteroduplex...including editors based on a second ADAR variant that displays improved specificity, and editors carrying an ... in vivo RNA imaging or to control alternative splicing. Figure 13: Overview of RNA targeting (A) and ...locus-specific targeting of long RNAs with CRISPR-Display. Nature Methods , 12 (7), 664–670. PMID: 26030444...

  40. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...following a G more efficiently than BE3, but APOBEC3G displayed less predictable sequence preferences.  The Liu...editors (ABEs). ABE7.10 is the most active editor, displaying an average editing efficiency of 53% with an ...of target positions 4-7. ABEs 6.3, 7.8, and 7.9 display slightly wider editing windows of position 4-9,...produce a mixed population of edits, ABEs do not display significant A to non-G conversion at target loci...Highlights Komor et al., 2016 BE1, BE2, BE3   BE3 displays highest editing efficiency but higher indel formation...

  41. Pooled Library Amplifications

    Type
    Blog Post
    ...from the Mark Dawson lab Re-transformation of the SPLINTR Libraries Protocol.   Selecting your strain...perform several individual transformations, such as splitting 400 ng of DNA in 100 μL of cells over four individual...Electroporation Protocol (DOCX, 15.8 KB) Barcode SPLINTR Pooled Libraries 200,000–1,200,000 60 bp ... 200 ng Liquid Re-transformation of the SPLINTR Libraries (PDF, 401 KB) Screening Zhang Lab...

  42. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be split into two halves, which are...proximity, they will form the full FP and fluoresce. Split FPs can be used to determine the proximity of two...two proteins fused to the halves of the split FP. This technique is also is also called Bimolecular Fluorescence...

  43. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...fusion proteins for imaging, tracking, modulating splicing, and regulating expression of specifically targeted...described in bacterial cells, LwaCas13a does not display nonspecific RNA cleavage activity in eukaryotic... LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant...to see this group of enzymes continue to make a splash in the CRISPR field. We feel that the culture of...

  44. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... of interest do not lie beyond the dimensions displayed in the plot. (B) A more restrictive gate results... second measurement to choose for graphically displaying your events. You are free to choose any size-... the positioning and shape of the populations displayed. As the separation pattern largely stays the same... cells but not dendritic cells. Using a graph displaying both stains, I can quickly separate T killer ...

  45. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...antibodies (rAbs) either produced de novo using yeast display technology or produced from hybridoma antibodies...The 11 IPI synthetic rAbs, developed using yeast display technology, are directed to integrins that bind...fragments (Fabs) that bound an amino acid region displayed by a subset of the RGD-binding integrins containing...Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits...

  46. Rett Syndrome

    Type
    Collection
    ...RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...2002. Insight into Rett syndrome: MeCP2 levels display tissue- and cell-specific differences and correlate...

  47. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...because the top figure shows the construct from our 'Split RNA' design, where the pre-processing form of crRNA... more details. One important note is that this ‘split RNA’ design is not as efficient in genome editing...

  48. Plan Your Experiment

    Type
    Collection
    ...should be made HDR requires a repair template and displays reduced efficiency compared to NHEJ knockout. ...region is removed from the mRNA due to alternative splicing. Exons near the N-terminus are targeted since ...

  49. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...proteins (PA-FPs) are fluorescent proteins that display unique changes in their spectral properties upon...population that is more neutral, causing PA-GFP to display almost no fluorescence when illuminated with 488nm...fact, many of the early reversible PA-FPs also display this “negative” photoswitching. In 2008, scientists...than mEos2 (mEos4b is completely monomeric) and display bright and photostable green and red states. Beyond...

  50. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc tag pPMW-attB - pUASp...expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular surface of S. ...
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