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We narrowed to 960 results for: App

Showing: 1 - 20 of 960 results

  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... Tempel 30137 pCAX APP 695 APP CAG Alzheimer's Dennis Selkoe 30138 pCAX APP 751 APP CAG Alzheimer's Dennis...Selkoe 30143 pCAX APP delta CT APP CAG Alzheimer's Dennis Selkoe 30144 pCAX APP AENATA APP CAG Alzheimer'...Selkoe 30145 pCAX APP Swe/Ind APP CAG Alzheimer's Dennis Selkoe 30146 pCAX APP C99 APP CAG Alzheimer's ... 30147 pCAX APPs-695-alpha APP CAG Alzheimer's Dennis Selkoe 30149 pCAX APPs-751-alpha APP CAG Alzheimer's... 196696 HA-APP-mGFP APP HA, GFP CMV Alzheimer's Martino Calamai 196704 mCherry-APP-mGFP APP GFP, mCherry...196694 mBFP-APP-mGFP APP tagBFP2, GFP CMV Alzheimer's Martino Calamai 196695 mBFP-APPP1-mGFP APP tagBFP2,...196705 mCherry-APPP1-mGFP APP GFP, mCherry CMV Alzheimer's Martino Calamai 196706 AP-APP APP Avi, HA CMV ...

  2. Neurodegeneration Research Collection

    Type
    Collection
    ...Rep Methods. 2022 Mar 28. Follow APP processing and dynamics using APP and Bace1 plasmids . Capitini et...inherited genes: Presenilin 1, Presenilin 2, and APP gene.The majority (>90%) of individuals develop late-onset...symptoms appear between a person’s thirties and mid-sixties, or late-onset, where symptoms appear during... these disorders. An integrated study of the overlapping biology of these diseases, rather than focusing...are still poorly understood but several genes and mapped loci seem to be associated with the disease and...

  3. 5 Great Apps for Lab Life

    Type
    Blog Post
    ... FREE science lab apps that have been educational and great for lab life. These apps prevent you from ...spectrometry app. I digress. When I started learning proper cell culture technique, I tried to find an app that...I came across the Gibco Cell Culture app. Gibco organizes its app into four categories: tools, analytics... Tools app comes in handy when I use protocols that require restriction enzymes or PCR. The app contains...Hence, I’m including this MSDS app. The Chemical Safety Data Sheets app has a search function that allows...of the many apps available to help researchers in the lab. If you have a favorite lab app, please share...by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article are those of the guest ...

  4. Antibody Guide

    Type
    Collection
    ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...of signaling molecules. Select one appropriate for your application and experimental design. Will you ...transcriptomics or proteomics. This approach is often appropriate for quantitative methods. Capture MS...protein of interest, with no overlapping epitopes. It is not appropriate for validation of an antibody...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...

  5. Immunology Research Plasmids and Resources

    Type
    Collection
    ...binding protein 1 CREB, MGC9284 CTSB cathepsin B APPS, CPSB CTSE cathepsin E CATE CTSL1 cathepsin L1 CATL...immunoglobulin kappa joining 1 J1 IGKJ2 immunoglobulin kappa joining 2 J2 IGKJ3 immunoglobulin kappa joining ...immunoglobulin kappa joining 4 J4 IGKJ5 immunoglobulin kappa joining 5 J5 IGKV@ immunoglobulin kappa variable...IGKDEL immunoglobulin kappa deleting element or like IGKDE IGKJ@ immunoglobulin kappa joining group IGKJ ...IGKV1-12 immunoglobulin kappa variable 1-12 IGKV112, L19 IGKV1-13 immunoglobulin kappa variable 1-13 (gene...IGKV1-16 immunoglobulin kappa variable 1-16 IGKV116, L1 IGKV1-17 immunoglobulin kappa variable 1-17 A30, IGKV117...IGKV1-27 immunoglobulin kappa variable 1-27 A20, IGKV127 IGKV1-33 immunoglobulin kappa variable 1-33 IGKV133...

  6. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...sfGFP David Spray 54959 mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Michael Davidson 55165...moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention signal oxBFP Erik Snapp 49150 BFP-KDEL...GolgiCFP Golgi apparatus eNOS(1-33) CFP Alexandra Newton 36205 pmTurquoise2-Golgi Golgi apparatus B4GALT1(1...68073 GalToxBFP Golgi apparatus GalT signal anchor transmembrane domain oxBFP Erik Snapp 158005 pCMV-mGold-MannII-N...10 Golgi apparatus MannII mGold Francois St-Pierre 85050 pmScarlet-i_Giantin_C1 Golgi apparatus Giantin...pmScarlet-H_Giantin_C1 Golgi apparatus Giantin mScarlet-H Dorus Gadella 85048 pmScarlet_Giantin_C1 Golgi apparatus Giantin ...Giantin Golgi apparatus Giantin mTurquoise2 Dorus Gadella 98880 mNeonGreen-Giantin Golgi apparatus Giantin ...

  7. CRISPR Guide

    Type
    Collection
    ... (2015). A scalable Genome-Editing-Based approach for mapping multiprotein complexes in human cells. Cell...technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) or transcription-activator-like...rapidly became the most popular genome engineering approach. Engineered CRISPR systems contain two components... to various Cas enzymes have extended CRISPR applications to increasingly complex functions, including...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR... Cas12a, which are available for specialized applications. Check out our list of additional Cas proteins...genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...therapeutic applications. Despite the ethical controversies surrounding non-research applications, it’s clear...Y. 2015. A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell...just as useful (if not more so) for research applications, eclipsing past genome engineering technologies...proteins snip foreign DNA into small fragments approximately 20 bp in length and paste them into contiguous...for CRISPR genomic editing. Genome Engineering Applications In 2012, Jinek et al. first demonstrated that...various species, as well as the development of new applications. The first CRISPR papers described two main ...RNA, making it a very flexible system. Other Applications Scientists have also used the targeting capability...

  9. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Bacterial Expression mApple 568 592 37 6.5 30 min Monomer mApple-N1 - Mammalian Expression mApple-C1 - Mammalian... Expression T-Sapphire 399 511 26 4.9 1.3 h Prone to dimerization pFA6a-link-yETSapphire-CaUra3 - Yeast...Yeast Expression mT-Sapphire 399 511 26 4.9 2.6 h Monomer (A206K) mT-Sapphire-N1 - Mammalian Expression ...Expression mT-Sapphire-C1 - Mammalian Expression mT-Sapphire-pBAD - Bacterial Expression LSSmGFP 400 510 18 4.7...page was originally compiled with the help of Erik Snapp . Note for all tables: Brightness is the product...Mammalian Expression mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-...and emission maxima (Stokes shift), useful for applications like multicolor imaging with better spectral...

  10. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...sure I missed some of your favorite apps. If you know of a software app that I missed, let me know in the... The software can be used with the ZappyLabs Bench Tools mobile app (available for iOS and Android). A...system does not appear to have a way to share your protocols with other groups. What apps do you use to ...publishers, and small start-ups are turning to software apps to solve our need for quicker, more effective updating...more to fit every lab's needs. Plus there are the apps - many to help you access your protocols at the ...comments below and leave your thoughts on how the app works for your lab’s needs. At Addgene, we’ll continue... some of our basic protocols to the protocols.io app so that all of our researchers can easily take those...

  11. AAV Packaged on Request

    Type
    Collection
    ...production will take approximately 6–8 weeks. Additional steps, including request approval, your institution...requests will be approved within two business days! Ordering At your convenience After approval, the next step...facilitation for the transfer plasmid, including legal approval and an implementation letter DNA amplification...institution’s approval of the MTA, and shipping, mean that the actual time from request to delivery could be up...before we can begin processing your order. MTA Approval 1+ business days, varies by requesting organization...remain subject to the terms and conditions of the applicable transfer plasmid. We will help you gain legal...

  12. PhD Applications After COVID

    Type
    Blog Post
    ...previous nosy question! The Application Process Broadly speaking, the PhD application process tends to go something...steps in the application process and usually occurs in the few months leading up to applications opening. ...program. Most applications will recommend that you name two professors in your application that you intend...to continue the application process through a campus visit, a weekend where applications can get to know...strengthen your application and help you decide if a program is a good fit for you. The application process looks...if you’re anything like me, the process of even applying to one of these programs is almost as daunting...be, at times, very overwhelming. As someone who applied to PhD programs in Biology around the United States...

  13. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...technique published every week! One of the newest applications is a colorful system that uses fluorescently...Thoru Pederson’s lab, brings us one step closer to mapping the 4D nucleome, the organization of the nucleus...activity, catalytically dead Cas9 (dCas9) has many applications, the most well-known being transcriptional activation... 9 and 13. They next turned their attention to mapping pairs of intrachromosomal loci. The technique successfully...authors’ knowledge, this work represents the first mapping of intrachromosomal loci, a major benchmark in ...characterizing the 4D nucleome. Future modifications and applications Ma et al. developed this technique using standard...for this technique. The NIH Common Fund has made mapping the 4D nucleome a specific priority, as researchers...

  14. Editor's Choice, September 2016

    Type
    Blog Post
    ...exhaustive list of all the apps out there, we encourage you to mention any apps you’ve found useful in the... other posts that deserve a second look. 5 Great Apps for Lab Life This very useful post was contributed...provides a brief rundown of Sean’s top five smartphone apps you can use in the lab. Whether you’re tired of ...that chemical in your obscure growth medium, these apps can help make your lab life a bit easier. Since ...

  15. All Antibodies

    Type
    Collection
    ...antibodies. These monoclonal antibodies undergo application-specific validation and quality control by Addgene...antigen. Addgene supplies a list of recommended applications based on our in-house testing and data provided...with full experimental details in the Antibody Applications section of our product pages. We also include... an antibody’s use is not recommended for an application or species. We currently assess western blot,...scientists to further develop and refine these application lists. The plasmids we use to produce antibodies...Source Species Isotype Reactivity Recommended Applications PI Return to Top Do you have suggestions for...

  16. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...their use in other clinical applications. In the last decade, viral applications in cancer treatment and ...s unpack the myriad number of emerging viral applications and give you some recommendations for putting...putting viruses to work. Viral applications Viruses work by infecting a host cell (the target of viral infection...expressed. It’s important to note that for all the applications we discuss, there is more than one type of virus...retrovirus, AAV, etc.). When you’ve decided on an application, our Viruses as Biological Tools blog can help...viruses and pick one for your experiment. Research applications Gene expression control Researchers are always...becoming increasingly popular tools with many applications. These libraries include sgRNAs targeting the...

  17. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...have been developed for different scientific applications. These PCR methods all use the same basic PCR...quantified by qPCR. qPCR is used in a variety of applications including gene expression profiling, studying...that fractions the PCR reaction sample into approximately 20,000 droplets. Each droplet contains the material...

  18. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ... typically occurring with a fluorescence-based approach. These challenges can include background autofluorescence...real time. Let's take a closer look at several applications of this technology. In each section below, we... attempts to purify or overexpress proteins inappropriate in discerning their true roles. Work conducted...context-dependent activity can render traditional tagging approaches insufficient. Vascular endothelial growth factor...

  19. Deisseroth INTRSECT Collection

    Type
    Collection
    ...proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach has been broadly...gene or projection). Intersectional expression approaches combine multiplexed recombinases, including Cre...broadly applied using multiple recombinase-expression strategies to define cellular sub-populations of interest... Oberhuber M, Conzelmann KK, Staiger JF. 2019. Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing... Dombeck DA, Deisseroth K, Awatramani R. 2018. Mapping projections of molecularly defined dopamine neuron...neuron subtypes using intersectional genetic approaches. Nat Neurosci. 21(9):1260-1271. PubMed (Link opens...

  20. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...function (Wood, et al). In the microbial world, approximately 50% of predicted genes have unidentified roles...endogenous gene. Each trap uses a slightly different approach (described in detail below), but fundamentally...redundant genes – multiple genes which function in overlapping roles can mask the depletion of one another. ...trap systems lack a promoter and thus require the trapping of an endogenous promoter. The system is composed...enhancer traps lack enhancer elements and require the trapping of endogenous enhancers to stimulate the expression...many more! Check out Addgene’s catalog to find trapping tools for your next experiment! References and...
Showing: 1 - 20 of 960 results