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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 352 results for: Stil

Showing: 1 - 20 of 352 results

  1. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...expression, though it is possible for the plasmid to still express without the G. Because the G is only one...the efficiency will be lower, although this might still be okay if you are introducing a selection marker...if/when you use HercII or Kapa Hifi the reaction still does NOT work, you can try these new primers: EMX1...

  2. TALEN Guide

    Type
    Collection
    ...but they still have potential targetable sites approximately every 35bp. Researchers are still determining...

  3. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... and Cascade on one single plasmid, though many still separate the individual Cas components. Figure 1...

  6. Plan Your Experiment

    Type
    Collection
    ...desired edit, as a large percentage of DSBs are still repaired by NHEJ. How do you determine that your...

  9. CRISPR Guide

    Type
    Collection
    ..., a large portion of the Cas9-induced DSBs will still be repaired via NHEJ. The resulting population of...

  10. Water Bath Protocol

    Type
    Protocol
    ...Equipment Water Bath 10% bleach or 70% ethanol Distilled water Disinfectant Thermometer Water bath weights...the edge of the slide. Fill the water bath with distilled water, not tap water, to prevent salts from accumulating...water in it from previous uses. However, you will still need to regularly clean out the water bath even ...

  12. Kit Free RNA Extraction

    Type
    Protocol
    ...entire tube is dried but while the white pellet is still visible. Resuspend RNA pellet in RNase-free water...

  13. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...solution by dissolving 40g of LB powder in 1L of distilled water. Autoclave and cool to 55°C. Add 1mL of ...of puromycin (Sigma-Aldrich catalog #P8833) in distilled water. Sterilize by passing through a 0.22 μm ...

  14. Centrifugation

    Type
    Protocol
    ...been placed in the centrifuge before you and may still be on the equipment. Before using the centrifuge...

  15. Fluorescence Titering Assay

    Type
    Protocol
    ...percentage of fluorescent cells, these cells can still be used in downstream applications. Safety Warnings...
Showing: 1 - 20 of 352 results