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We narrowed to 862 results for: LIC

Showing: 451 - 500 of 862 results

  1. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...Janssen Award dinner which took place at the New York Public library. Being in the presence of scientific heroines...adaptive bacterial immunity and its potential applications in rewriting the genome. Their award acceptance... area will accelerate the launch of clinical applications. During the panel discussion, Dr. Mello took...

  2. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...tools available for bacteria and some of the applications for which they’ve been used. The beginnings ... E. coli. Furthermore, to demonstrate wider applicability they demonstrated success of Cas9 mediated repression...as a collection of organisms, their genes, and metabolic processes, occupying the gut. Many human diseases...scenario allows for several potential CRISPR applications. First, CRISPR can be used to target locations...

  3. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...to sdAbs becoming valuable tools for diverse applications. For example, RANbodies can be used as an alternative... excitement around using sdAbs in diagnostic applications. For example, fluorescently and radioactively...inspired this versatile family of tools, the applications they have already enabled, and the discoveries...

  4. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...: Find sequences for plasmids assembled from publications and commercially available sources, as well ...making it ready to copy and paste into other applications Primer design tools Many of the above-listed... well as primer design for different cloning applications. Primer3 and Primer3Plus: For general primer...

  5. Hot Plasmids - October 2022

    Type
    Blog Post
    ...+ over other Na+ and other cations. B) Cortical slice of HcKCR1-EYFP and tdTomato expressed layer 2/3 ...blot, but we are actively testing it in other applications! If you try it out in a new assay, let us know...GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc [9E10] page for more...

  6. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...should be. He debated taking a position as an application support/sales representative in industry or a...communicator than a science writer these days. I do some public speaking and a bit of video work for webinars (...illustrations for summarizing mechanisms and applications that are relevant to things IDT does. I also...

  8. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ... Specifically, viruses are able to infect and replicate in host cells, but they are susceptible to cells... though not all, of these defenses. For some applications of genome engineering - such as certain forms...to integrate. Additionally, the TA sequence is duplicated near the gene of interest insertion site.   The...

  9. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ... writing, lab budgeting, equipment acquisition, public speaking and personnel management?  If you are ...ask a lot of questions. Personality and style conflicts are a common reason for scientists to change labs...websites listing alumi and it is easy to use publication records, university websites, ResearchGate and...

  10. Deisseroth INTRSECT Collection

    Type
    Collection
    ... mouse and rat lines. The Fenno Lab maintains a public folder (Link opens in a new window) of manuscripts...

  12. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ... be used as a fusion tag for a wide range of applications from traditional imaging to FRET and stochastic...fluorescent protein: structural characterization and applications in fluorescence imaging." Biochemical Journal...identification of the molecular determinants of color and applications in fluorescence imaging." BMC biology 6.1 (2008...

  13. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents...

  14. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...diphtheria toxin, indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness...

  15. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...brain, which has humanistic and philosophical implications. As an example, a group at Caltech used an optical...a handle on some of these mysteries. Another application is clinical; can we find ways to treat patients...available. Visit Ed Boyden lab's Addgene page and click the link in the Addgene Alerts box. References...

  16. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...2017). Beyond human relevance, yeast have many applications in food science and biotech due to its fermentation... Glucose Glucose, methanol, glycerol Heterothallic mating Efficient Efficient Inefficient... ancient yeast strains and metazoan cells.   Applications for K. phaffii There’s a number of different...

  18. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...improve our shipment processes. After reading a publication describing how short term storage on dry ice ...closed dry ice storage container for 48 hours (to replicate how our viruses are shipped), where they would...exposed to CO2/dry ice. All samples were tested in duplicate. What we found Not all tubes (or rather, not ...

  19. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...formation. The quest for plant compounds to treat complications of diabetes Gloria Otito Izu, PhD Candidate...nephropathy, one of the major pathophysiological complications of diabetes mellitus. Recombinant human proteins...Biology of the NationalAcademy of Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior...

  20. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...playing a card game called “Magic”. There were complicated rules that governed how this game was played.... as I try being compelling, interactive, and application-driven to boost curiosity, I have never experienced... cards (Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection Markers) and one...

  21. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...packaged into viral vectors for potential in vivo applications or multiplex CRISPR screening.   Using tRNAs...and efficient in plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided...construct DAP array for multiplex gene editing applications, we provide detailed manual and online design...

  22. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...actuators that could be used in a variety of applications. For example, you could use them to lyse cells...matrices) in response to pH changes. Beyond these applications, purified R bodies could also perform as actuators...PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer...

  23. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...altering its function). HDR is crucial for certain applications, for example, correcting the allele that causes...nucleofection The in vivo editing experiments in this publication were performed using a technique called nucleofection...Additonal Corn Lab Members who worked on this publication:         References 1. Richardson, Christopher...

  24. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...properties, previously established uses, and intended applications. Within CasPEDIA, there is a classification ...multiplex ability Experimental guidelines and applications Protein structure and properties Links to ...you want to read literature on the less common applications of an enzyme for your non-traditional experiment...

  25. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...demonstrated that pathways projecting to the inferior colliculus (IC) are differentially transduced when using...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ...

  26. How Addgene Creates Content

    Type
    Blog Post
    ...antibody and content team, we identified what applications should be included and what basic information...through an editing process and final check before publication.   Do research! Nearly everything we write entails...make figures in BioRender or use appropriately licensed and cited figures from papers or image sharing...

  28. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...the key components of EvolvR and its potential applications. Challenges of existing mutagenesis methods...increasing the standard mutation rate seen during DNA replication by 120-fold over background. Due to its modular...more than one mutation near the target site. Applications of EvolvR The Dueber Lab used EvolvR to discover...

  29. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...their reagents with the scientific community.   (click to enlarge) As we look ahead to the things to come...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify...

  30. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...cells and antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...

  31. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades.  So let’s break ...transmembrane domain receptors, due to the seven alpha helices that transverse the cellular membrane. The receptor...engineered receptors to interact with small molecules to elicit a response in a certain cell type. Chemogenetics...

  32. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...as they do science outreach and engage with the public, and learn from the process of writing papers and...broader impacts for their work in education and policy and to draw attention and support to work in their...before you complete your work. If you’re giving a public talk, ask a friend who works in a different field...

  33. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...sequences. Fusion PCR was performed to create an amplicon that  was cloned into a plasmid backbone for expression...to make the sequences of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative...Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded their recombinant...

  35. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement...self-process pre-crRNA into crRNA). The size and simplicity of Cas12a makes it easier to package for therapeutic... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main...

  36. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...methionine. The labeled proteins can be detected using click-chemistry that covalently links the azido containing... observed with fluorescence microscopy. This application of AHA labeling is known as fluorescence noncanonical...

  37. RNA Extraction Without A Kit

    Type
    Blog Post
    ...of DNA that can potentially complicate results of a downstream application such as quantitative PCR to...is to precipitate the RNA using lithium chloride. LiCl solutions selectively precipitate RNA, but not DNA...

  38. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...help answer? Ramanathan et al. outline three applications of RaPID: Discovery. To discover novel RNA-... that QKI is a host protein critical to Zika replication. Human genetic disease. RaPID can also be used...

  39. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...neuroscience method that lets you fire neurons with the flick of a light switch. Neurons are not typically persuaded... expression in cultured neurons and mouse brain slices collected following intracranial injection of an... as compared to direct injection. This lower multiplicity of infection (MOI) makes it challenging for ...

  40. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...and in the intersection between science and public policy. Follow David on Twitter @driglar.     References...professor of economics at the Andrew Young School of Policy Studies at Georgia State University, is an expert...

  41. Working with Nuclear Receptors

    Type
    Blog Post
    ...the body through the bloodstream, and eventually elicit their actions on other tissues. At the Nuclear ...a broad range of reproductive, developmental, metabolic, and immune response programs. NRs also encompass...gallstone disease, senescent illnesses, and the metabolic syndrome. Coregulators Coregulators constitute...

  42. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...genotype and phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You...imagine, there is an array of distinct goals within metabolic engineering, synthetic product production, and...

  43. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ... reduced off-target editing frequencies. For applications that require high DNA editing specificity, for...deamination, an important attribute for therapeutic application. ABE8s demonstrate an overall improved base editing...editors with increased activity and therapeutic application. Nature Biotechnology. https://doi.org/10.1038...

  44. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ... when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites blocked by dCas9....for dCas9 to interfere with DNMT1 even through replication. The dCas9 also needs to be removeable, otherwise...doi.org/10.1038/s41467-021-25991-9. Spruijt CG, Gnerlich F, Smits AH et al. (2013) Dynamic readers for ...

  45. Tips for Technical Support Calls

    Type
    Blog Post
    ...originally described in a peer-reviewed scientific publication. We love science, but we don’t have the resources...be fairly fast, getting the full answer to a complicated question may take some time. If the customer ...message Answering customer service questions is a delicate balance of knowledge, patience, and diplomacy,...

  46. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...hypomethylation, on the other hand, has also been implicated in the development and progression of cancer ...challenge was difficult to overcome until now. Alice Ting’s lab has developed a new, plasmid-based biosensor...:12404098.  4. Lin, Chi-Wang, Cindy Y. Jao, and Alice Y. Ting. "Genetically encoded fluorescent reporters...

  47. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...recently there has been a counter movement by the public to access basic equipment and to become involved...first hackerspace which now offers members and the public cheap access to personal manufacturing tools – ...woodworking tools, and a metalshop. After two summers in Silicon Valley as a student and then instructor at Singularity...

  48. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ... and limited the exchange of information to the public and to other academic labs. Being unable to publish... organisms, and GMOs have a bad reputation. The public is can be fearful of this technology and angry ...something new and beautiful, to engage with the public in a meaningful way about science, and to have ...

  49. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...something you may have to optimize for your specific application. With mammalian systems, it is standard to do...to collect your viral supernatant. Baculovirus replicates inside insect cells, and its lifecycle ends in...titer the virus, or go straight into your viral application, like protein production. Titration of baculovirus...

  50. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...instances. If your poster material is nearing publication, consider uploading your work to a preprint server...conference If the conference has a social media policy, this should be on their website or the program...program booklet. Make sure you’re familiar with the policies before posting something on social media. If someone...
Showing: 451 - 500 of 862 results