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We narrowed to 967 results for: Cre

Showing: 181 - 200 of 967 results

  1. Antibody Guide

    Type
    Collection
    ... cell cultures using hybridomas, cell cultures created by fusing B cells with myeloma cells (immortalized...immortalized B cells). The genetic reorganization that creates variety in the antibody allowing for their extreme...animals immunized as described above are used to create hybridomas, which produce large amounts of a single...recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma...goes through a randomized reorganization process, creating a unique binding site. This process, known as ...roles in the immune response, and can be used to increase flexibility or specificity in antibody-based methods... Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

  2. COVID-19 Resources

    Type
    Collection
    ...Plasmid Tools Pooled Libraries Other Resources Image credit: Maya Kostman for the Innovative Genomics Institute...), including plasmids for protein production, secretion, and codon-optimized versions designed...metalloprotease that cleaves hACE2, potentially increasing viral infectivity (Heurich et al., 2014). PCP4...-CoV-2 Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead Libraries of Spike (S) Ectodomain...Binding Domain Deep Mutation Scanning Library Screening Jesse Bloom Library of Spike protein RBD mutations... Domain Site-Saturation Mutagenesis Libraries Screening Jesse Bloom Tyler Starr Libraries of variant Spike...Beating Affected by the Pcp4 Dose-Dependent [Ca2+]i Increase in Down Syndrome Mice, Ts1Rhr . International ...

  3. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

  4. Antibody Plasmid Collection

    Type
    Collection
    ...synthetically produced recombinant antibodies, created by cloning antibody components into plasmids and...mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected for...smaller fragment of an antibody, which could offer increased stability and ease of production due to their ...highlights plasmid collections that can be used to create and express plasmid-based antibodies. Description...domains to obtain recombinant hybridomas, which secrete antibodies as Fab' fragments, isotype-switched ...antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining...

  5. Distribution to Industry

    Type
    Collection
    ...Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created for research in Parkinson's Disease.... How to Order Creating an Account: Before placing your order, you will need to create an account and ...for cloning and assembling standardized parts to create eukaryotic multigene constructs. ONE-GO Biosensors... our website. Placing an Order: After you have created an account, you can place orders through our website...

  6. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

  7. CRISPR Plasmids - Plants

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

  8. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...factor isoform in pooled screens. This collection and library can be used for screening transcription factors...available at Addgene. The collection can be used for screening transcription factors for cellular programming...window) . TF ORF barcode PCR for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X...bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...

  9. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

  10. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins from...Subcellular Localization General Cytoskeletal Junctions Secretory Pathway More Resources The subcellular localization... shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding... or search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting...

  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Expression HcRed1 588 618 0.3 Dimer pCAG-HcRed - Mammalian Expression HcRed1-N1 - Mammalian Expression...plasmid backbones with different fluorescent tags to create fusion proteins with your gene of interest. Plasmid...Expression HcRed1-C1 - Mammalian Expression dKatushka 588 635 22 5.5 Dimer dKatushka-pBAD - Bacterial Expression...community-editable database for fluorescent proteins created by Talley Lambert (Link opens in a new window) ...

  12. NETRF

    Type
    Collection
    ...carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF believes that creating and maintaining... accelerate scientific discovery that will help create new and more effective therapies for these tumors...States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

  13. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  14. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...CRISPR plasmids that create double strand breaks in DNA....Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  15. Institute for Protein Innovation

    Type
    Collection
    ...antibodies targeting families of cell surface and secreted proteins from a collaboration between Addgene ...antibodies targeting families of cell surface and secreted proteins (glypicans and integrins) and common ...common epitope tags, as well as antibody plasmids to create or express plasmid-based antibodies. The majority... Plasmids The following plasmids can be used to create or express plasmid-based antibodies. ID Plasmid...

  16. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...activity. The variety in sequence decreases the gRNA activity and increases off-target effect. Which species...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...requirements, experimental considerations, and more, created and maintained by the Doudna Lab . gRNA Plasmids...

  17. TALEN Plasmids and Kits

    Type
    Collection
    ...next generation architecture has been shown to increase mutation induction when using TALENs. The FokI...Gersbach These new destination vectors can be used to create TALE-transcription factors and are used at the ...TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence...these plasmids along with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation...

  18. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  19. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

  20. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target alterations and... Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or search the...
Showing: 181 - 200 of 967 results