... using CRISPR AAV transduction CRISPR elements are inserted into an AAV transfer vector and used to generate...stable cell lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of... in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP...generate AAV particles (for details, see our AAV Guide ) ∼4.5 kb packaging limit (only compatible with...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...desired vector. In many cases, targeting oligos are synthesized, annealed, and inserted into plasmids containing...particular model organism. There may not be a perfect plasmid for your specific application, and in such cases...

...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis...

...Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections Background... None Either Elledge 35625 pAAV-Ptet-RFP-shR-rtTA AAV; shRNA cloning vector; for evaluation of shRNA efficacy...interest tTA expression plasmid Components for a tetracycline on system are: Plasmid containing a tetracycline-dependent...generation lentiviral plasmid for inducible expression of shRNA; neomycin selection; plasmid 21915 has puromycin...pInducer20 Lentiviral vector for shRNA expression; see article for additional tookit plasmids third-generation...expression vector, CMV promoter, and Blasticidin; can be used to make cell lines Tet-On Advanced; Plasmid 26730...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Background... bacterial expression vector (see tagged expression vectors from the SGC) pPro18...expression vector (see entire pPro collection ) Arrowsmith Lab Plasmids - More...More LIC bacterial cloning vectors pTD plasmid series - Expression with...Sandmeyer Lab Plasmids - Set of yeast expression vectors with various markers... plasmids Fly UAS, MT Targeted Gene Expression - Rubin lab plasmids for drosophila...gene promoters C. elegans vector kit - Collection of plasmids from Dr. Andrew Fire L4440...

...Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors Fluorescent...links to find the plasmids associated with the article. We also offer ready-to-use AAV preparations of ...Living Cells", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type PI Publication Return to Top...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...of select plasmids; these are noted with the viral service icon . Metal Ions Target Description Article...

...are single preparations of many different plasmids. Plasmids within a given library have the same backbone...about various scientific concepts related to our plasmid collection. Find links to the guides below. Antibodies...Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced by the immune system, antibodies...restricted to certain gene sets. Read More Viral Vectors Find educational guides to learn more about the...research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus Read More...

...Restriction Cloning Plasmids 101: Inducible Promoters Plasmids 101: What is a plasmid? Plasmids 101: Antibiotic...Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids E. coli strains ...propagating plasmids Antibiotics commonly used for plasmid selection DNA sequencing for plasmid verification...to be studied into the plasmid. Below are some common plasmid elements: Plasmid Element Description Origin...Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...biology basics and plasmid components. Plasmids 101: Origin of Replication Plasmids 101: The Promoter ...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...

... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells... table based on plasmid name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available...information page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...RNA p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive...

...Broad GPP genome-wide Brunello 73179 (1 plasmid) 73178 (2 plasmid) Knockout Human Doench and Root 3rd 4 ...76,441 Broad GPP genome-wide Brie 73632 (1 plasmid) 73633 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 (2 plasmid) Knockout Human Doench and Root 3rd... 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...20,051 Human GeCKO v2 1000000048 (1 plasmid) 1000000049 (2 plasmid) Knockout Human Zhang 3rd 6 123,411...185,836 Mouse GeCKO v2 1000000052 (1 plasmid) 1000000053 (2 plasmid) Knockout Mouse Zhang 3rd 6 130,209... - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716 SAM v2 - 2 plasmid system...

...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...Recombinases Addgene ID Plasmid Logic Sites and Mutations 55636 pAAV-EF1a-Cre None 55632 pAAV-Ef1a-mCherry-IRES-Cre...recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations 55641 pAAV-Ef1a-fDIO EYFP Flp 55640 pAAV-Ef1a-dDIO hChR2...Addgene ID Plasmid Logic Sites and Mutations 55650 pAAV-hSyn Con/Fon EYFP Cre AND Flp 231926 pAAV-Ef1a-Con...Addgene ID Plasmid Logic Sites and Mutations 137119 pAAV-EF1a-Con/Fon-GCaMP6M Cre AND Flp 137120 pAAV-Ef1a-...Addgene ID Plasmid Logic Sites and Mutations 131779 pAAV-nEF-Con/Fon TVA-mCherry Cre AND Flp pAAV-nEF-Con...

...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease ...population of lentiviral transfer vectors. In most cases, each plasmid contains an individual gRNA targeting...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included... included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...

...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease ...population of lentiviral transfer vectors. In most cases, each plasmid contains an individual gRNA targeting...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included... included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...

...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor...contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for targeting vector assembly,...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt...proteins. This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms...targeting the genomic site and the donor vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked...donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh donor plasmid. Note...

...Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection... GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast...Browse all Neurodegeneration Plasmids Do you have suggestions for other plasmids that should be added to this...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic...others. The table below can be sorted by disease, plasmid type, mutations, and/or tags such as fluorescent...particular gene of interest, please search our full plasmid collection using the search bar at the top of this...

... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You...Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional Resources Mammalian...to the available plasmids for each gene. Please note that not all genes have plasmids available for request...and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for each gene...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...rapidly initiate a secondary immune response. Browse Plasmids for Immunology Research Genes have been grouped...