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We narrowed to 412 results for: his

Showing: 181 - 200 of 412 results
  1. Hot Plasmids - August 2020

    Type
    Blog Post
    ...created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly used for in vivo biotinylation... localization signal or including fusions with Histone 2A or 2B can result in bright targeted expression... desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be ...
  2. Lab to Office Culture Shock

    Type
    Blog Post
    ...Big thank you to Jessica Welch, Mike Lemieux (for his advice and for modeling in the picture above), and...
  3. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...ChIP-seq Proteins (for our example later, histones) ChiRP-seq RNA Hi-C DNA (genome-to-genome interactions...bound to modified proteins called histones. We can detect modified histones with “GPS for proteins,” ChIP-... of modified histones, a genomic region that has mono-methylated and acetylated histones, but not tri-...tri-methylated histones, functions as an enhancer. We can therefore denote enhancers as follows: (mono_methylation...of epigenomics: H3 – one of NANOG’s associated histone proteins K4 and K27 – locations of the amino ...combination of coiled DNA and structural proteins called histones. Chromatin folds into secondary structures (loops...
  4. The Challenges of Cell Culture

    Type
    Blog Post
    ...AstraZeneca-MedImmune, and Infors & Applikon. Due to his high level of experience he now travels around Europe...and money but cells lose provenance (this is the history of the cell line consisting of records of the origin...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...
  5. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...expression of the HIS3 gene, which is required for histidine manufacture and therefore growth on his-deficient...that repress instead of activate transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait... is an example of the former. In this system, histidine-auxotrophic host strains are used to select for...
  6. Antibody Guide

    Type
    Collection
    ..., or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...methods such as immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use... they only have one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can... can refer to both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (...
  7. Gibson Assembly Protocol

    Type
    Protocol
    ...TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly of DNA...Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis...
  8. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...frequent bouts with Impostor Syndrome, Joe has found his people through meetings and social media. Joe is ..., and the Society for Advancement of Chicanos/Hispanics and Native Americans in Science’s (SACNAS) annual... career scientists, students, and those from historically underrepresented groups. Through such mentoring...
  9. CRISPR Library Amplification

    Type
    Protocol
    ...Petri dish (VWR, 11019-552) 4 MaxiPreps (Qiagen HiSpeed Max, Catalog #12663) Tips (1000 µL, 200 µL, 10 ... Maxiprep. Purify plasmid DNA using the Qiagen HiSpeed Maxi Kit (one conical is its own Maxiprep). Critical...
Showing: 181 - 200 of 412 results