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We narrowed to 413 results for: his

Showing: 181 - 200 of 413 results

  1. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...ChIP-seq Proteins (for our example later, histones) ChiRP-seq RNA Hi-C DNA (genome-to-genome interactions...bound to modified proteins called histones. We can detect modified histones with “GPS for proteins,” ChIP-... of modified histones, a genomic region that has mono-methylated and acetylated histones, but not tri-...tri-methylated histones, functions as an enhancer. We can therefore denote enhancers as follows: (mono_methylation...of epigenomics: H3 – one of NANOG’s associated histone proteins K4 and K27 – locations of the amino ...combination of coiled DNA and structural proteins called histones. Chromatin folds into secondary structures (loops...

  2. The Challenges of Cell Culture

    Type
    Blog Post
    ...AstraZeneca-MedImmune, and Infors & Applikon. Due to his high level of experience he now travels around Europe...and money but cells lose provenance (this is the history of the cell line consisting of records of the origin...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...

  3. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...expression of the HIS3 gene, which is required for histidine manufacture and therefore growth on his-deficient...that repress instead of activate transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait... is an example of the former. In this system, histidine-auxotrophic host strains are used to select for...

  6. Antibody Guide

    Type
    Guide
    ..., or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...methods such as immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use... they only have one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can... can refer to both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (...

  7. Gibson Assembly Protocol

    Type
    Protocol
    ...TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly of DNA...Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis...

  11. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...frequent bouts with Impostor Syndrome, Joe has found his people through meetings and social media. Joe is ..., and the Society for Advancement of Chicanos/Hispanics and Native Americans in Science’s (SACNAS) annual... career scientists, students, and those from historically underrepresented groups. Through such mentoring...

  15. CRISPR Library Amplification

    Type
    Protocol
    ...Petri dish (VWR, 11019-552) 4 MaxiPreps (Qiagen HiSpeed Max, Catalog #12663) Tips (1000 µL, 200 µL, 10 ... Maxiprep. Purify plasmid DNA using the Qiagen HiSpeed Maxi Kit (one conical is its own Maxiprep). Critical...

  17. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...to fluorophores. Examples are the FlAsH tag, the His tag and several others. Another peptide-based strategy...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...

  18. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... lab of Arlene Sharpe at Harvard Medical School. His projects include investigating negative regulators...of such targets include transcription factors, histones, and DNA repair enzymes. Note that when using ...stepwise decrease in fluorescence intensity in a (histogram) flow plot (Figure 4b). Through this method, differences...

  19. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... lab of Arlene Sharpe at Harvard Medical School. His projects include investigating negative regulators...respectively. H, A, and W describe the shape of a histogram, which graphically represents the time and intensity...graph of photocurrent vs. time. The resulting histograms is described through maximum current (H), the...
Showing: 181 - 200 of 413 results