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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 482 results for: vent

Showing: 181 - 200 of 482 results

  2. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...regardless of end structure, and dictate the major events of the pathway: Broken ends are recognized by ... probably accounting for less than 5% of repair events. However, the products of accurate repair are easily...

  4. To Each HIS Own

    Type
    Blog Post
    ...post-purification cleavage step to remove the tag in order to prevent it interfering with protein function. In contrast...with the protein of interest (Bornhorst 2000). To prevent this, wash steps often include low concentrations...

  6. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...in iPSCs, but with the caveat of mosaicism. To circumvent this problem, they co-injected CRISPR machinery...germline editing is permissible in cases of disease prevention. However, it is important to consider that already-tested...

  7. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...their male mice died—and they didn’t know why. Eventually, through a Gendered Innovations workshop in Norway...ensures that the diagnostics and therapeutic interventions you develop represent the whole human population...

  8. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...symposium. The talks and workshops held at this event added to a growing dialogue on the state of a system...Boston 2015 you can register here or live stream the event on October 22nd and 23rd here. Many thanks to our...

  9. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...will reduce noise and ensure you are measuring the events-of-interest. Sensors suitable for ER measurements...nucleus-targeted GCaMP6 with an extra apoCaM-binding motif to prevent aberrant binding of the biosensor to endogenous...

  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...enter (and thus infect) cells, this safety feature prevents the virus from replicating and spreading in non-target...Types of Viruses Viral Vector Elements Tips for Preventing Viral Recombination  ...

  11. A Look at Addgene's QC Process

    Type
    Blog Post
    ...points at which small errors can be introduced. Eventually, these errors could cause the plasmid to stop...caught prior to distribution. Catching these errors prevents future experimental issues, saving time and money...

  12. Design Tips for Prime Editing

    Type
    Blog Post
    ...Edit the PAM along with your intended edit. This prevents the Cas9 nickase from re-binding and nicking the... al., 2019). Figure 4: Editing the PAM prevents the prime editor from re-engaging with DNA it ...

  14. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...endogenous regulatory control. But even with the advent of CRISPR/Cas9 technology, inserting large tags...found within the homology areas of the plasmid to prevent the plasmid from being cut and destroyed by CRISPR...

  16. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... site due its proximity to the cleavage site, preventing future edits. In contrast, since Cpf1 cleaves...mediate HDR in a “second chance” mechanism. To prevent new editing post-HDR, repair templates should remove...

  17. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...fiber optic for the implant depends on the dorsal/ventral location of your region of stimulation (the z-axis...need to be polished to significant smoothness to prevent drastic loss of efficiency between patch cable ...

  18. Of Myc and Men

    Type
    Blog Post
    ...especially in the mid-late 20th century with the advent of hybridoma technology and monoclonal antibodies...Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–...

  19. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...many many times in a short time period. PCR was invented in the early 1980s by Kary B. Mullis who later...to design PCR primers.  Types of PCR Since the invention of PCR, different PCR methods have been developed...

  20. Common Injection Routes in Mice

    Type
    Blog Post
    ...as this method is useful for analyzing localized events. Intradermal injections require a bit of extra ...through a feeding tube with a bulb on the end, which prevents accidental tears or punctures to the throat. The...
Showing: 181 - 200 of 482 results