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We narrowed to 862 results for: LIC

Showing: 501 - 550 of 862 results

  1. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...instances. If your poster material is nearing publication, consider uploading your work to a preprint server...conference If the conference has a social media policy, this should be on their website or the program...program booklet. Make sure you’re familiar with the policies before posting something on social media. If someone...

  2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...the genome and is propagated during normal DNA replication and cell division. Mobile-CRISPRi does not disrupt...plasmids, and lack the ability to synthesize diaminopimelic acid (DAP), a component of the cell wall. Once...single-gene knockdown experiments. Mobile-CRISPRi applications Mobile-CRISPRi expands CRISPRi to non-model ...

  3. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...do this internally, but they are not available publicly. Furthermore, we did not find any tools which ...with NGS data were: Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca Pinello ...the How To Guide. Conclusion CrispyCrunch is now publicly-available for high-throughput design and analysis...

  4. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...something you may have to optimize for your specific application. With mammalian systems, it is standard to do...to collect your viral supernatant. Baculovirus replicates inside insect cells, and its lifecycle ends in...titer the virus, or go straight into your viral application, like protein production. Titration of baculovirus...

  5. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...been excitedly testing NgAgo's genome editing applications. So, how does NgAgo stack up thus far? A few..., those who have been lucky with NgAgo should explicitly share tips and protocol details with the scientific... S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell...

  7. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or... used a solution of benzyl alcohol and methyl salicylate which was later modified by others to produce...Spalteholz. (1914) Uber das Durchsichtigmachen von menschlichen und tierischen Praparaten. Leipzig: S. Hierzel...

  8. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ...

  9. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...your experiment. Shorter amplicons tend to be better. Shorter amplicons make for shorter PCR programs...Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional ...

  10. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...manuscript to get reviewed and edited prior to publication, and this will delay the dissemination of your... makes it easy to annotate protocols, make them public for others to use, and add your own comments. Currently...Freedom: The Expansion of Data Sharing in Research Publications by Jim Woodgett        ...

  11. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...detrimental as too little, resulting in MECP2 duplication syndrome or Rett Syndrome, respectively. One ...editing, MECP2 reactivation, RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts....0.co;2-o Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY (2013) An AT-Hook Domain in MeCP2...

  12. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...experimental design and its potential for clinical applications. Specifically, efficacy rates of Cas7-11 were...place, while increasing the number of potential applications. But they weren’t done engineering quite yet... at the bench and potentially in therapeutic applications. The potential of CRISPR has yet to fully taken...

  13. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Protein A and Protein G are not suitable for all applications, such as protein isolation from serum. In this...capture antibody interaction and/or the downstream application. Typically, if the target protein will be detected...and an isotype control antibody. Alternative applications Oftentimes, an IP-appropriate antibody simply...

  14. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...pooled library DNA, allow the bacteria to grow and replicate, then purify the DNA.     Figure 2: A pooled...protocol. I also recommend searching PubMed for any publications associated with the library and reading their...no more than one viral particle, giving a low multiplicity of infection (MOI). MOI is controlled by viral...

  15. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...affected by many experimental factors. To list some complicating factors for quantifying AAV: qPCR efficiency...8610.4. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, Vessella RL, Tewari M... abundant targets: from variable nonsense to publication quality data." Scientific Reports 7 (2017). PubMed...

  16. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...our loved ones, and become a positive force for public health. Visit Addgene's Microbiology Pages What...Microbes (bacteria included) are very skilled at the delicate art of survival. Existing virtually ubiquitously...about health equity, science communication, and public health.     References 1. O'Neill, J. "Tackling...

  17. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...study its function is one of the most popular applications of fluorescent proteins. These fusion proteins...linker sequence short In the early days of GFP applications, many were concerned with steric hindrance by...between secondary structure (beta-sheets or alpha helices). An additional requirement is that the site of...

  18. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...genomic DNA in many cell types and often results in biallelic modifications. Most commonly, DSBs are repaired.... Furthermore, CRISPR/Cas9 editing is often bi-allelic, meaning that even if one allele is accurately ...heterozygous mutations by multiplying the single allelic mutation incorporation probabilities our data revealed...

  19. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...strand. While this pathway may not be immediately applicable in DSB-induced genome engineering, it has biological...that can be harnessed by researchers for many applications. Your favorite Cas enzyme, a guide RNA to your...Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014)...

  20. Evolution of Lab Techniques

    Type
    Blog Post
    ...comparison to a control sample. Southern blotting applications range from confirming the results of cloning...sequence thousands of reads at the same time. Other applications of sequencing, such as confirming the results...replaced these, these techniques still have useful applications and have informed many current techniques. For...

  21. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...stimulation. GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms...break your experiments!  First, closely read the publication, including the supplemental data. You’re most... both of which have extensive collections of publications and information on hardware, optogenetics tools...

  22. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...source of piggyBac™ transposase (available from a licensed distributor) allow you to quickly transfer a DNA...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...technology that may be used for a variety of research applications, including making transgenic cell lines and ...

  23. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...genetic code of living organisms and its societal implications even before the discovery of genes themselves... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...us to always consider the ethics and broader implications of our research.  Want the Genome Editing Trading...

  24. Important Considerations When Using AAVs

    Type
    Blog Post
    ...vivo animal model should consider the possible elicited immune response from viral infection. Animal safety...rate limiting step and can immediately undergo replication and transcription. However, with scAAV the effective...only can we compare these methods in specific applications, we may also be able to use these comparisons...

  25. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...editing typically needs to be optimized for each application, and the efficacy varies widely — it still almost...editing variants Four years after their initial publication, the Liu lab returned to the reverse transcriptase...Image from Doman et al., 2023, under a CC BY 4.0 license. epegRNAs: Structured RNAs with improved ...

  26. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...that the antigen being detected in the intended application — here, flow cytometry — is the desired target...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi.org/10.7554...of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. eLife...

  27. New Antibodies in the Lab Animation!

    Type
    Blog Post
    ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

  28. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...trick. Just flip, and press! Lab tip #2: Tube flick trick When combining reagents in the lab, it is ...the various reagents to a single tube and simply flick the tube several times. This will help ensure ensure...

  29. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ...from Bruce Wetzel and Harry Schaefer, NCI, NIH | License   I’m updating it regularly as people send me suggestions...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points...

  31. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...

  32. Call for Guest Bloggers

    Type
    Blog Post
    ...minipreps? If so, we’d love to have you write for us! Click Here to Contribute to the Addgene Blog Possible ...molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development...

  33. Summer Fun at Addgene!

    Type
    Blog Post
    ... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...

  34. Editor's Choice, July 2016

    Type
    Blog Post
    ...target and use FRET flurophores for a variety of applications. While editing this post, I learned a lot about...in their own research. All posts from July 2016 Click on the links below to read all of our excellent ...

  36. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...will simply load an extra ladder or technical replicate (ie, leftover sample) to fill the relevant wells...using an antibody validated for your protein and application of interest or validating an antibody yourself...lysis Add sonication step to protein lysis Splice variants Check literature Posttranslational...

  38. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ... lend them many other functions for use in RNA splicing, imaging, and more. Abil says she is currently...says the new tool might even find therapeutic application in addition to their use in basic biology, for...

  39. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ... (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the business” Attend...Academic Solos to Industrial Symphonies by Gwen Acton, Alicia Gómez-Yafal & Emily Walsh The Hard Truth About ...

  40. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ... of 17 signaling pathways that are frequently implicated in cancer cell proliferation, survival, differentiation... make the library usable for pooled screens. Applications of the cancer pathways ORFs kit Wood and his...

  41. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...introduction to pCri, please see the original publication cited below. pCri plasmids are available either...comprehensive review of affinity tags and microbial applications." Biotechnology journal 7.5 (2012): 620-634....

  42. Science as Art

    Type
    Blog Post
    ... new method to explore the inner structures of delicate plants and other organisms without destroying ...competition. This competition has gained so much public interest for its stunning images that is has been...

  43. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...complete with all the fixings), as well as plenty of delicious desserts. We enjoyed a video highlighting the ...course of scientific research. What was once a complicated process of requesting and sharing plasmids has...

  45. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF.... Sensitivity In ex vivo studies on mouse brain slices, nLightG and GRABNE were more sensitive than nLightR...

  48. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...of hues. While those tools are great for many applications, Verkhusha and his lab at Albert Einstein College...Verkhusha’s team reported another advance in the application of near-infrared fluorescent proteins, iRFP670...

  50. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ... mutations in single stranded DNA undergoing replication.   DNase on the other hand can digest and degrade...directly use your DNA preparation in any downstream application involving enzymatic reactions, a valid alternative...
Showing: 501 - 550 of 862 results