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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 882 results for: NSI

Showing: 501 - 550 of 882 results

  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...gels. They are shared through Addgene without licensing restrictions. Use these instructions to prepare... genome, many applications in biotech, and is considered the most studied Gram-positive bacterium, many...University of Minnesota recently deposited a comprehensive set of plasmids that can be used for either ...

  2. Kit Free RNA Extraction

    Type
    Protocol
    ...tissues, extraction of RNA, precipitation, and resuspension. This protocol provides two options: using Solution...phase without disturbing the interphase layer, consider using a lower volume pipettor like a p200 to collect... freeze-thaw cycles of your entire RNA sample, consider making smaller aliquots of it and storing those...

  3. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...you plan PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically...Additional Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great Tools for Gene...

  4. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...AAV Packaged on Request, an expansion of our viral vectors service, has officially launched! As of today... excited for all the ways this much-requested expansion will allow us to better serve our community. If...

  5. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...developed a platform called SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single...further to provide multiplexed detection, higher sensitivity, and more. Read the paper in Science Find...

  6. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ... hard about picking a good mentor, and begin considering career options early. Tune in for great advice...year dip (see graph). Niroshi and Ben give their insights as to what causes this dip. 11:11 - 15:37 : How...

  7. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ...journals has risen steadily from 1995 to 2013. Considering citations to all articles, Anurag Acharya and...their own libraries and offering another way to consider and quantify the impact of scientific publications...

  8. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...exemplifies our long-standing commitment to be responsive to the community’s needs and our commitment to...many exciting things planned in 2023, from the expansion of our Data Hub to process improvements in every...

  10. Water Bath Protocol

    Type
    Protocol
    ...solution or a 70% ethanol solution to wipe down the inside of the water bath. Pro-Tip To properly maintain...maximum fill capacity is. Look for a “fill” line inside the water bath or read the user manual. If you’...may float in the water bath when they are placed inside. Water bath floats can be used to secure tubes ...before use, make sure that there is enough water inside....

  11. Science Communication for Everyone

    Type
    Blog Post
    ... It is important to identify your audience and consider their background. Social media is a powerful tool...what you are trying to convey without having to extensively use Google search. Be careful about hidden connotations...

  13. Let There Be LITE Plasmids

    Type
    Blog Post
    ... another level with the addition of two light-sensitive ingredients - CRY2 and CIB1 – which they borrowed...advantages of the new system: "First, the light-responsive molecules of cryptochrome 2 are the chromophores...

  14. Video Library

    Type
    Protocol
    ...Addgene's online resources. Career Videos Get career insight and advice from real Addgenies! Protocols (Link... once using a multichannel pipette and avoid inconsistent pipetting with our simple tips Bacterial Transformations...Australia, and advice for current graduate students considering their future careers. Eric J. Perkins, PhD In...Addgene's Business Development Manager, discusses his transition from the Scientist Team to Business Team, his...

  16. Editor's Choice, August 2016

    Type
    Blog Post
    ...CRISPR-based technique to generate C->T or G->A transitions in a target sequence in live cells. This technique...endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by gRNAs. Caveats...

  17. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages...for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the tetraloop of the...

  20. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ... the spine. For binders of protocols and data, consider using color coded binders and tabs (for different...to protocols in real time. You may also want to consider moving your paper spreadsheets for reagent storage...

  21. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ... check to make sure that it isn't methylation sensitive . Plasmids grown in Dam or Dcm methylase...opens in a new window) NEB's table of methylation sensitive restriction sites . Sometimes enzymes cut sequences...diagnostic digest), you can create a "Master Mix" consisting of all of the reaction components except for ...each tube. This will save you time and ensure consistency across the reactions....

  23. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag...

  24. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...instead results in the breakage of the ester bonds responsible for the sugar backbone of the DNA helix or even...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...

  25. Fostering communication in remote collaborations

    Type
    Blog Post
    ...a time. Just be sure there is a point person responsible for incorporating everyone’s suggestions into...timelines for experiments and manuscripts, who is responsible for which parts of the project, and, as mentioned...

  28. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...researchers around the world. We take this responsibility seriously. Every decision we make, every project... provides unmatched customer service that is consistently timely, informative, friendly, and professional...

  29. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...generally follow the same pattern described above - a sensing domain induces changes in a fluorescent signal ...compatible with your lab and experimental setup? Consider what kind of instrumentation you have available...

  30. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...a separate plasmid. This system amplifies the intensity of the fluorescent signal and enables single molecule...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...

  31. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...cupcakes, or maybe the lab member originally responsible for the deposit had already moved on to their...with journal articles. But we do encourage you to consider sharing them openly as soon as that preprint is...

  34. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...Chance to do/learn new things, diversity of responsibilities, which might include training or teaching ...Nature Biotechnology article “Managing knowledge-intensive workers”. I have added a few comments of my own...

  35. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...most applications. Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media...store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. Keep in ...

  37. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...perfect fit for Addgene. She was Head of Quality at Dimension Therapeutics, which was acquired by Ultragenyx... in 2017. As one of the original employees at Dimension Therapeutics, she helped build and grow the gene...

  41. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...communication, I had the chance to carry out a comprehensive review of the literature informing the notion...science. Understanding just how the public utilizes insights gleaned from scientific research is, after all...

  42. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...find useful antibodies for your research. Please consider sharing your experiences with antibodies from .... The content of this article is solely the responsibility of the authors and does not necessarily represent...

  45. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...important as its capabilities are the practical considerations of actually using it - the device has a rechargeable...expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain ...

  46. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...our early stage.  After each pitch we made, we considered the feedback and adjusted our business model ...company) and is our money guy (CFO).  As CEO, I am responsible for internal operations and building external...

  47. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...iTOP is best suited to binary systems in which transient protein expression has a long-lasting effect, ...in the genome. Second, expression is by nature transient, which may lower nuclease toxicity or the potential...

  48. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...impact your work? What design variables need consideration? Enrich your knowledge and skills outside of...network Attend informational interviews to gain insight into the daily routine of the potential job Join...

  49. Pipetting Protocol

    Type
    Protocol
    ...1 mL). When working in a laboratory, properly dispensing liquid ensures the accuracy of your experiment...experiment and any changes to the amount you are dispensing can negatively impact your experimental results. ...the liquid and avoid touching the pipette to the inside of the tube. Only the tip should be in the liquid...different tubes or into wells of a microplate, you may consider using a multichannel pipette. Please watch Addgene...

  50. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... Everyone. Plan out new experiments carefully. Consider submitting a registered report, where peer review...is based on the methods, not the results. Also consider using this framework to design your future experiments...
Showing: 501 - 550 of 882 results