We narrowed to 217 results for: Apple fluorescent protein

... transgenes. It’s important to note that the fluorescence level observed will be dependent on both the...lies in the diversity of nucleases and their applications. It will be interesting to see what new editing...Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human Cells.” Cell Rep. 13: 621...

...well as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc...BBB via interactions with a similar protein called LY6C1. This protein is expressed in both BALB/cJ mice...exists to prevent substances in the blood, like proteins and viruses, from crossing into the brain. While... it, cross the BBB through an endothelial cell protein called LY6A (Hordeaux et al., 2019; Huang et al...capsids that rely on interactions with those two proteins to cross the BBB are often not effective in NHPs... selected for its ability to bind to the LY6C1 protein in order to reduce strain-specific tropism in mice... W. L., & Wilson, J. M. (2019). The GPI-Linked Protein LY6A Drives AAV-PHP.B Transport across the Blood-Brain...

...antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot Abi? If so, you...When assembled, the four chains make a Y-shaped protein that can be divided into the Fragment Crystallizable...the fragment could bind not only the endogenous proteins you are trying to block but your primary antibody...the antibody you do and don’t need, and how the protein will interact with other components of your experiment... as secondary antibodies, have much broader applicability. It is fairly easy to find these in a Fab fragment...pre-form primary-secondary antibody complexes before applying them to samples, such as when performing IHC in...can be used as a block in similar scenarios. By applying unconjugated Fab fragments that are the same species...

...components including (1) a CRISPR RNA, (2) four proteins forming the QCascade DNA-targeting module with...with the crRNA, (3) three transposase proteins, and (4) the donor DNA. By using a multi-spacer CRISPR array...Zalatan’s lab used an approach to identify known proteins involved in the activation of transcription including...including transcription factors, phage proteins, and RNAP components (Dong et al., 2018). They found a...dCas9 variant fused to bacterial enhancer binding protein required for the activation of sigma-54 type promoters...glycosylase inhibitor and degradation tag on the fusion protein to limit the activity of the system as a whole....tools available for bacteria and some of the applications for which they’ve been used. The beginnings ...

...unreliability: cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently...atlases/literature and is unrelated to the target protein, we categorize the antibody as having non-specific...the best, or even which one will work for your application? Like many scientists, I have spent hours sifting...produced from a new set of animals), and wrong application (different experiments/conditions can change ...differently over time), compatible with the desired applications (such as immunohistochemistry (IHC) or western...secondary antibodies, allowing us to only image autofluorescence i.e., background noise. We then compare the...

...Overlay Select Fluorescent Proteins 101 August 2017 | First Edition About Fluorescent Proteins 101 August...for choosing a fluorescent protein; information on FRET, biosensors, and monitoring protein localization...localization; an introduction to non-protein fluorophores; and much more! Close About Overlay Select Viral Vectors...contains an introduction to each vector and its applications, tips for using vectors in your experiments,...your transferable skills, and tips on the job application process. Close About Overlay Select CRISPR 101...introductions and helpful tips for antibody-based applications, from flow to IHC! Close About Overlay 0 of ...

...assembling bimolecular fluorescence complementation experiments to analyze protein-protein interactions in plants..., synthetic terminators, protein purification tags and fluorescence proteins for use in E. coli for a ...Dupont 95 plasmids including backbones, fluorescent proteins, protein tags, and signal peptides, for use in...elements and fluorescent reporters to be used in combination with the MoClo-YTK toolkit for protein expression...example, a promoter, a 5' UTR, a signal peptide, a protein-coding gene, and a terminator, as in Figure 1)....PhytoBrick parts. MoBiFC - Modular Bimolecular Fluorescence Complementation Toolkit Plant Expression Benjamin...optimize secretion and cell surface display of proteins of interest. Yeast Secrete and Detect Yeast Expression...

...the instructions to make every protein within an organism. Before proteins can be translated, the DNA is...RNA (mRNA), which is then translated into proteins. Proteins are made up of amino acids. Each amino acid...control of a non-bacterial promoter or a fluorescent protein to select or sort the cells by visualization...of transcription driven by that promoter. Fluorescent Protein Plasmids , Luciferase Plasmids Viral Plasmids...Have diverse applications — Although plasmids were originally used to understand protein coding gene function...discoveries led to the conclusion that genes encode proteins and reside on chromosomes, which are composed ...in the central dogma of molecular biology, that proteins are translated from RNA, which is transcribed ...

...Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent Proteins 101 Science Career Guide Videos Watch our...techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid Technology ...written and video protocols for a wide range of applications in molecular biology, plasmid cloning, and virus...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...specificity and applicability of base editing through protein engineering and protein delivery. Nature...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ..., or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...

...done using Protein A, Protein G, or the recombinant Protein A/G, which are bacterial proteins that bind...amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein complexes...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...to the protein of interest. Signal strength is determined by how many antibodies bind the protein of interest...amounts of protein. Western blot Western blots use antibodies to identify denatured proteins separated ...primarily show a protein’s presence or absence. Figure 3: A western blot detecting proteins using the indirect...

... vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein with broad tropism used to pseudotype...infectivity VSV-G (vesicular stomatitis virus G protein) is the most common envelope gene, due to a wide...resistance is not the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection...marker. Instead of selection by an antibiotic, fluorescence-activated cell sorting (FACS) is used to sort...RNA packaging signal; recognized by nucleocapsid proteins and essential for efficient viral packaging. cPPT...Packaging plasmid gag in trans Precursor structural protein of the retroviral particle containing matrix, capsid...nucleocapsid components. pol in trans Precursor protein containing reverse transcriptase and integrase ...

...release proteins from cellular membranes using light. light-induced protein dissociation using protein A fragments...Photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein 400 UVR8 domains...photoreceptors to create protein systems controlled by light. These "photoswitchable" proteins offer exquisite ...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...Inhibits protein function by reversibly sequestering targets into large, optically assembled protein architectures...transcription using engineered zinc finger proteins, using proteins GIGANTEA (GI) and the light oxygen voltage...pair to the target protein, and the other to the membrane where the target protein is to be sequestered...

...the Rev protein binds. Psi (Ѱ) in cis RNA packaging signal; recognized by nucleocapsid proteins and essential... vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein with broad tropism used to pseudotype...infectivity) VSV-G (vesicular stomatitis virus G protein) is the most common envelope gene in all systems...resistance is not the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection...marker. Instead of selection by an antibiotic, fluorescence-activated cell sorting (FACS) is used to sort...third-generation systems do not require the HIV protein Tat to activate the promoter on the transfer plasmid...Packaging plasmid gag in trans Precursor structural protein of the lentiviral particle containing matrix, capsid...

...plasmid type, mutations, and/or tags such as fluorescent protein (FP) tags. Information on genes and associated...Davidson 54924 mApple-MAPTau-C-10 MAPT mApple CMV Parkinson's, FTD Michael Davidson 54925 mApple-MAPTau-N-10...Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy, ALS...-10 MAPT mApple CMV Parkinson's, FTD Michael Davidson 55077 mCherry-MAPTau-C-10 MAPT mCherry CMV Parkinson's... hnRNPA2_LC_WT HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98658 hnRNPA2_LC_D290V HNRNPA2B1...

...transmembrane proteins that bind a specific ligand. Ligand binding then activates G-proteins to modulate...bioluminescent modules, such as luciferase-fluorescent protein fusions, to more-sensitive opsins has led...The first chemogenetic receptors were based on G-protein coupled receptors (GPCRs) . The largest class of...DREADDs), like RASSLs, are based on engineered G-protein coupled receptors, but unlike RASSLs, show insensitivity...that can be broadly classified by the signaling protein that the receptors couple to: Gq-DREADDs Gq-DREADDs...Gq-DREADDs signal through the Gαq/11 G-protein and activate neuronal firing through stimulating phospholipase...-DREADDs Gi-DREADDs signal through the Gαi/o G-protein and inhibit neuronal signaling by inhibiting adenylate...

...region encodes three structural capsid proteins or viral proteins (VP1, VP2 and VP3) that form the icosahedral... vectors have been engineered to express fluorescent proteins, often under strong neuron-specific promoters... split inteins, proteins that carry out a naturally occurring process known as protein trans-splicing.... the viral particle and are only composed of a protein capsid and the viral genetic material inside. The...region encodes four non-structural replication proteins (Rep78, Rep68, Rep52, and Rep40), which are involved...chromosome 19. This integration is mediated by proteins Rep78 and Rep68, which can bind to specific Rep-binding...not carry the genes to express Rep78 and Rep 68 proteins and are therefore unable to integrate in the host...