We narrowed to 430 results for: ERG
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TypeBlog Post... to tell if the dependent variables worked synergistically, antagonistically, were additive, etc.). ANCOVA... The Bonferroni Correction and the Benjamini-Hochberg procedure are designed to reduce false positive...
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9 tips for a successful postdoctoral experience
TypeBlog Post...learning. Serve as a daily mentor for a summer undergrad, rotating grad student or high school student.... the time to develop strategies to rest and re-energize yourself, and to address all of the other parts... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...Rensselaer Polytechnic Institute. He also studies energy markets in his spare time. You can follow him on...are not present at the lab bench," Liu, a UM undergraduate, challenged us. I worried the march would be... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...hypopigmentation, thus marking cells that have undergone genomic modification. In this system, Cas9 must...plasmids from this paper at Addgene. 8. Xie, Kabin, Minkenberg, Bastian, & Yinong Yang. “Boosting CRISPR/Cas9... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...generations of optimization before the field begins to converge on a set of standard tools. Additionally, aspiring...Base Editing. Advanced Science (Weinheim, Baden-Wurttemberg, Germany), 9(26), e2202957. https://doi.org/10.1002... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...editor proteins, but found that the mutations that emerged depended strongly on the target edit (Doman et .... E., Holden, K., Ma, H., Xie, J., Gao, G., … Gootenberg, J. S. (2023). Drag-and-drop genome insertion... -
Antibody Validation for Flow Cytometry
TypeBlog Post...Bandrowski, A., Carr, S., Edwards, A., Ellenberg, J., Lundberg, E., Rimm, D. L., Rodriguez, H., Hiltke... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...sgRNA-scaffold 47912 Worm AflII none S. pyogenes Sternberg pJW1311 61254 Worm none, fusion PCR none S. pyogenes... -
Antibody Guide
TypeCollection...against parasites and is responsible for driving allergic reactions such as anaphylactic shock Monomer with... -
Using AAV for Neuronal Tracing
TypeBlog Post...in only specific cell types, for example only dopaminergic neurons, neuronal tracing has the added benefit...798–810. PubMed PMID: 92475. Taymans, J. M., Vandenberghe, L. H., Haute, C. V., Thiry, I., Deroose, C.... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...compartment, you may want to try an SDS-containing detergent like RIPA. You can try a few different lysing ...background noise is high, you can consider adding a detergent like Tween-20 to your washes — although this can... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...mitochondrial degradation in vitro and in vivo. Merged green and red channel fluorescence images of C2C12...effectively cuts the target sequence, the plasmid undergoes HR or SSA to reconstitute functional GFP, and ... -
27 Hot Plasmids from 2016
TypeBlog Post... et al. Science. 2013. PubMed PMID: 24136966 Nyerges, et al. Proc Natl Acad Sci USA. 2016. PubMed PMID...Zurovec pBlue-TAL Nathan Lawson pJDS Series Superglue proteins with SpyTag/SpyCatcher and SnoopTag/SnoopCatcher... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog Post...Toolbox Hot Plasmid 2: AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new... -
The 12 Days of CRISPR
TypeBlog Post...day of CRISPR, Addgene gave to me: RADARS from Gootenberg, Chen, and Abudayyeh🎵On the 9th day of CRISPR... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog Post...Hardware? Author: Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog Post...dvpr (lentiviral) or pUMVC (MuLV retroviral). Bob Weinberg pMD2.G VSV-G envelope expressing plasmid Didier... -
DIY DNA Ladders from Penn State University
TypeBlog Post... recently deposited with Addgene. A team of undergraduate students led by Dr. Song Tan at Penn State developed... -
8 Great Depositor Research Stories
TypeBlog Post...Cas7-11 and PASTE: Omar Abudayyeh and Jonathan Gootenberg worked with us to share their new CRISPR tools... -
New Viral Vectors - March 2024
TypeBlog Post... a biosensor that detects both serotonin and serotonergic drugs. Check out these new vectors, and many...