We narrowed to 363 results for: gne
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TypeBlog Post...pressure to make sure everyone completes their assigned items to keep the project moving. Use time wisely...
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Hot Plasmids - May 2022
TypeBlog Post...other experiments. And, while the BLITZ system was designed and optimized with zebrafish in mind, it can easily... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...differentiation in a high-throughput manner. They designed the pXPR_053 lentiviral expression vector, which... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post... shift requires deliberate preparation and a willingness to embrace new responsibilities. After my second... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post..., the use of LIC is often limited to specially designed plasmids. Sequence- and ligation-independent cloning... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post... that you know how to read flow plots and have designed your first flow panel, you’ll load your samples... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post... also show you how multiplexed arrays would be designed, if that’s your end goal. CasPEDIA also features... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...References 1. Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...target cells and organisms, and when that cargo is designed to make you sick…well, you feel it. But the same... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...annotations throughout the map at locations better aligned to their precise positions within the plasmid. ... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...signatures or cancer phenotypes. These assays were designed to complement the one assay per gene approach ... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post.... Similar to a jigsaw puzzle, the oligos were designed with sticky ends that only anneal together in ... -
Plasmids 101: Inducible Promoters
TypeBlog Post... popular light-regulated two component systems designed by the Tabor lab. Which inducible promoter is ... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...came to the Addgene booth (there were over 400 who signed up for our newsletter and a free plasmid) were ... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...Bhopal. All illustrations / figures featured were designed by our team member Kallol Mozumdar. Sreepadmanabh... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...purification is now a reality thanks to OptoNBs. Recently designed by the Toettcher Lab, OptoNBs are photoswitchable... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...synbodies--or synthetic antibodies--attached to magnetic beads, allowing the virus to be easily concentrated... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...Addgene page went up last month, over 340 people have signed up to receive alerts when plasmids become available... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...the plasmids made by Xie et. al are specifically designed for plant expression, PTGs will likely be adaptable... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...first generation of CRISPR-Cas9 plasmids were not designed with gene activation in mind. “We had not managed...