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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 517 results for: pes

Showing: 201 - 220 of 517 results

  1. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...on the search page to quickly jump to different types of items: Did you notice the “Filter By…” options...The search bar will suggest direct links to other types of searches including depositing labs and institutions...can’t browse through 300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of...

  2. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...repressing target genes in a variety of species and cell types can be found on Addgene’s website. Activating target...activating target genes in a variety of species and cell types can be found on Addgene’s website. Fig. 3...optimized for expression in different species and cell types including (but not limited to) bacteria, yeast, ...

  3. Hot Plasmids - November 2023

    Type
    Blog Post
    ...surface, expanding the range of targetable cell types. DIRECTED is compatible with fusogens from different...such as lentivirus and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible...Lin D., Li X., et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature Biotechnology...

  4. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...Pooled libraries Scientists have deposited many types of pooled libraries here at Addgene, including cDNA...Addgene are lentiviral pooled libraries, though other types exist.  When you buy a pooled library from Addgene...Spinfection transduction efficiency can vary across cell types and generally requires optimization of many variables...

  5. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ... a gene in a living animal across multiple cell types using a mouse model for a human neurological disease...the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology has formed... improves survival and ameliorates behavioral phenotypes in MeCP2 null (S51.002). Neurology 92 (15 Supplement...

  7. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...cell types are used. The lack of good flow cytometric antibodies for many CNS-resident cell types (microglia...applied to the differentiation of other CNS cell types, including microglia, astrocytes, and oligodendrocytes...

  8. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...groups and departments. You don’t have to get these types of projects and groups going alone - if you are ... discipline. Getting companies to come to these types of events can be very tough. First I needed to find...growth through my interactions with many different types of people. As mentioned in the beginning, the ability...

  9. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...revolutionize neuroscience Manipulation of specific cell types in neuronal networks Double inverted vectors expressing...only means to target and manipulate specific cell types (populations) within neuronal networks in living...Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit Mapping.” Journal...

  10. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...expression at certain loci to create changes in phenotypes. For example, CRISPR activation has been used...and Gersbach, 2015; Zetsche et al., 2015). These types of CRISPR activation are also reversible: once the...et al., 2016). To learn more about the different types of Cas9 activators, check out our blog post covering...

  11. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...There are many, many different types of experiments carried out by scientists every day. Although the...plasmids would address and answer these questions. Types of control plasmids Part of planning your experiment...expressing the independent variable. There are two types of replicate controls: technical and biological....

  12. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...require interaction with the data authors. In some types of research, raw data requires huge amounts of storage...calling it a “sisyphean task” given the endless types of data). Rather, PLOS appears to leave the scope...provide data in formats exported to common file types? And how long should data be archived? Simplifying...

  13. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Because transcription machinery differs between cell types or organisms, promoters must be similarly variable...derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc) require unique promoters...an enhancer region. Can be silenced in some cell types. EF1a General expression mRNA Strong mammalian...

  14. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...DNA viruses, such as adeno-associated virus, herpesviruses, hepatitis B virus, adenoviruses, and human ...linear proviral DNA is poised for integration, two types of circular episomes can be generated through either...This makes IDLVs an attractive choice for certain types of clinical or basic research applications. Considerations...

  15. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...bind to epitopes of interest that are found in the extracellular space. The targeted epitopes can be motifs...simply need to label the lineage markers of all cell types except CD8+ T cells. We might choose the markers...

  16. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...lymphocyte population from a tumor sample. Many cell types can be found in the tumor microenvironment (tumor...Around 34% of the cells are neither of the two cell types (likely B cells and others). Almost no cells stain...in this blog post. First, you learned about the types of scattering that occur when a cell is pulsed by...

  17. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...specific 3-dimensional epitopes. Different sample processing steps can alter epitopes in different ways, ...my cells,” or “How does this mutation impact the types of cells found in my tissue.” It is based on the...

  18. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...to admit that paper-based record keeping barely copes with the growing amounts of digital data generated...functionalities with experiment records. Many different types of ELNs are available on the market today, ranging...showed that It is important that an ELN supports all types of data, which enables researchers to store everything...

  19. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...target site sequence at the site of insertion.  Types of transposons Transposable elements are split into...the size of their host’s genome.   There are two types of Class 1 TEs: those with long terminal repeats... used in the lab While there are many different types of transposons, DNA transposons are most commonly...

  20. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ... knock-in efficiency across different stem cell types. This has facilitated easy generation of clonal ...choice for epitope tagging in diverse stem cell types. Hundreds of genes can be epitope-tagged in a couple...large protein cohorts in diverse mammalian cell types. A detailed step by step protocol for the csRNP ...
Showing: 201 - 220 of 517 results