...proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral drip or... For detailed protocols, volumes, spin speeds, recipes, and Agrobacterium strains see Weigel and Glazebrook...occurs in a coding region, it may cause unexpected phenotypes. For most phenotypic analysis it is appropriate...

... multi-gene knockouts in a wide variety of cell types, gene editing using homology directed repair (HDR...internalization of Cas9-gRNA RNPs by specific cell types (Rouet et al. 2018). This was accomplished using...additional factors to take into account with these types of experiments. One important consideration is that...

...groups and departments. You don’t have to get these types of projects and groups going alone - if you are ... discipline. Getting companies to come to these types of events can be very tough. First I needed to find...growth through my interactions with many different types of people. As mentioned in the beginning, the ability...

...otherwise normal cell types are produced, rather than novel, abnormal cell types. Sequencing-based lineage...spending months generating mutants. Unexpectedly, phenotypes sometimes differ depending on whether a gene ...

...cell types are used. The lack of good flow cytometric antibodies for many CNS-resident cell types (microglia...applied to the differentiation of other CNS cell types, including microglia, astrocytes, and oligodendrocytes...

...repressing target genes in a variety of species and cell types can be found on Addgene’s website. Activating target...activating target genes in a variety of species and cell types can be found on Addgene’s website. Fig. 3...optimized for expression in different species and cell types including (but not limited to) bacteria, yeast, ...

... a gene in a living animal across multiple cell types using a mouse model for a human neurological disease...the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology has formed... improves survival and ameliorates behavioral phenotypes in MeCP2 null (S51.002). Neurology 92 (15 Supplement...

...revolutionize neuroscience Manipulation of specific cell types in neuronal networks Double inverted vectors expressing...only means to target and manipulate specific cell types (populations) within neuronal networks in living...Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit Mapping.” Journal...

...surface, expanding the range of targetable cell types. DIRECTED is compatible with fusogens from different...such as lentivirus and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible...Lin D., Li X., et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature Biotechnology...

...Pooled libraries Scientists have deposited many types of pooled libraries here at Addgene, including cDNA...Addgene are lentiviral pooled libraries, though other types exist.  When you buy a pooled library from Addgene...Spinfection transduction efficiency can vary across cell types and generally requires optimization of many variables...

...expression at certain loci to create changes in phenotypes. For example, CRISPR activation has been used...and Gersbach, 2015; Zetsche et al., 2015). These types of CRISPR activation are also reversible: once the...et al., 2016). To learn more about the different types of Cas9 activators, check out our blog post covering...

...There are many, many different types of experiments carried out by scientists every day. Although the...plasmids would address and answer these questions. Types of control plasmids Part of planning your experiment...expressing the independent variable. There are two types of replicate controls: technical and biological....

...require interaction with the data authors. In some types of research, raw data requires huge amounts of storage...calling it a “sisyphean task” given the endless types of data). Rather, PLOS appears to leave the scope...provide data in formats exported to common file types? And how long should data be archived? Simplifying...

...Because transcription machinery differs between cell types or organisms, promoters must be similarly variable...derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc) require unique promoters...an enhancer region. Can be silenced in some cell types. EF1a General expression mRNA Strong mammalian...

...bind to epitopes of interest that are found in the extracellular space. The targeted epitopes can be motifs...simply need to label the lineage markers of all cell types except CD8+ T cells. We might choose the markers...

...lymphocyte population from a tumor sample. Many cell types can be found in the tumor microenvironment (tumor...Around 34% of the cells are neither of the two cell types (likely B cells and others). Almost no cells stain...in this blog post. First, you learned about the types of scattering that occur when a cell is pulsed by...

...to admit that paper-based record keeping barely copes with the growing amounts of digital data generated...functionalities with experiment records. Many different types of ELNs are available on the market today, ranging...showed that It is important that an ELN supports all types of data, which enables researchers to store everything...

...specific 3-dimensional epitopes. Different sample processing steps can alter epitopes in different ways, ...my cells,” or “How does this mutation impact the types of cells found in my tissue.” It is based on the...

...target site sequence at the site of insertion.  Types of transposons Transposable elements are split into...the size of their host’s genome.   There are two types of Class 1 TEs: those with long terminal repeats... used in the lab While there are many different types of transposons, DNA transposons are most commonly...

...is induced by various types of interactions with their environment. These types of interactions to a large...growth properties, migration abilities, or other phenotypes that can be efficiently measured at the single...