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We narrowed to 482 results for: vent

Showing: 201 - 220 of 482 results

  1. Of Myc and Men

    Type
    Blog Post
    ...especially in the mid-late 20th century with the advent of hybridoma technology and monoclonal antibodies...Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–...

  2. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...many many times in a short time period. PCR was invented in the early 1980s by Kary B. Mullis who later...to design PCR primers.  Types of PCR Since the invention of PCR, different PCR methods have been developed...

  3. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...at the 5’ cap so that only a single translation event occurs for each mRNA. However, some bicistronic ...ITAMs ensures effective negative selection and prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun...

  4. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...equally effective are shipped to customers on ice, preventing any temperature shifts. Of course, we are also...cautious during the aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced...

  6. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...and Pol, increasing the number of recombination events necessary to create a replication-competent virus... vectors contain a deletion in the 3’ LTR that prevents aberrant activation of nearby genes. These safer...

  7. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...site due to its proximity to the cleavage site, preventing future edits. In contrast, since Cpf1 cleaves... has not yet been experimentally confirmed. To prevent new editing post-HDR, repair templates should remove...

  9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... structure, shielded from quenching by aqueous solvent. This tightly-packed structure explains the importance...mutations are acceptable. GFP's main advantage over conventional fluorescent dyes of the time was the fact that...

  10. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...targets with high specificity and affinity, were eventually called aptamers, from the Latin aptus, meaning...presence of that metabolite (Fig.2). Spinach eventually evolved into Spinach2, a more stable and brighter...

  11. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...In this blog post, we will cover three ways to circumvent this limitation: 1) the use of novel S. pyogenes...-type SpCas9. The number of off-target cleavage events for the VQR and VRER variants is similar to wild-type...

  12. Bioinformatics at Addgene

    Type
    Blog Post
    ...accompanying sequencing data collection and analysis eventually outpaced our software, even with continuous updates...containing our new process) on our local computer. Eventually, our workflow would handle plasmid sequences ...

  13. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...we give people the opportunity to speak out and prevent groupthink? Here are some ideas that I have found...our lab space, our protocols and even our Lab Inventory Management system. I hope these tips help you ...

  14. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...species and isotype of their primary antibody. To circumvent this, a recombinant protein, Protein A/G, was...phosphatases that may degrade the target protein. To prevent this, include protease and phosphatase inhibitors...

  17. How to Write a Scientific Review Article

    Type
    Blog Post
    ... the beginning I felt like this was cheating. Eventually I learned that it’s practical because there simply...how elusive a free two hour block of time was. Eventually, I learned that I was most productive in short...

  19. The Challenges of Cell Culture

    Type
    Blog Post
    ... in a separate backup liquid nitrogen store to prevent loss from equipment malfunctions. One Master vial...approach, the many mistakes of the past can be prevented and we can progress faster to desired clinical...

  20. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...stimulating an ill-defined region of the brain. With the advent of optogenetics, ICSS can now be done with neuronal...Differential control of learning and anxiety along the dorsoventral axis of the dentate gyrus." Neuron 77.5 (2013...
Showing: 201 - 220 of 482 results