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Addgene

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We narrowed to 545 results for: SEC

Showing: 221 - 240 of 545 results

  1. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ... of a bigger movement in a variety of research sectors to provide open source technologies and spaces ...bacterial species in the gut of the domesticated housecat, and seeing how that makeup differs from that ...

  2. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...in what is called a reeling mechanism (see next section for details). The newly formed single-stranded ...that the two breakpoints will repair without the section between the two gRNAs, creating a deletion. However...

  3. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...John P. S., Basic Methods, Human Stem Cell Manual (Second  Edition), 2012. Additional Resources on the Addgene...

  4. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ... reads should align to this reference sequence. Second, we perform a megaBLAST search on all the reads...vectors for your research. Please use the comments section below to let us know if you have any specific questions...

  6. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...target sequence for U6 promoter expression and the second site accepts the homology arm repair template. ...fusion of the desired gRNA to the U6 promoter, and a second plasmid containing the homology arms and an N- ...

  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification...production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized and ...

  10. DNA Purification Without a Kit

    Type
    Blog Post
    ...plants, microbes, blood or mammalian cells in 30 seconds or less without using a pipette.  Here’s how this... from plants, animals and microbes in under 30 seconds. PLoS biology. https://doi.org/10.1371/journal....

  11. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...topic. For example, it may be useful to adapt the second version (multiple presenter, figure by figure) ... seemed to be of use, it’s worth scheduling the second and other subsequent meetings. The number of participants...

  13. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...necessary to create a replication-competent virus. Second, Tat is eliminated through the addition of a chimeric...us know how you use lentivirus in the comments section below! References 1. In vivo gene delivery and...

  14. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...-like endonuclease domain, but they lack Cas9’s second HNH endonuclease domain. Cpf1 cleaves DNA in a ...to cleave again and possibly mediate HDR. This “second chance” mechanism might improve the frequency of...

  16. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...results of any experiment I’ve run in under thirty seconds.” That’s exactly what you would want to talk about...possible, send it to a mentor or colleague for a second look and helpful feedback!  What if I’m not a good...

  17. How to Be an Excellent Trainee

    Type
    Blog Post
    ...shared server. The blue box labeled “A5” on the second rack in the corner freezer sounds easy to remember... some things I could pay attention to, so my dissections go smoother next time?” or more generally “How...

  20. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...keeping in touch by email and social media (see the section on “Maintaining Relationships” below). Expand: ...learning and, in my experience, mistakes and insecurities are more easily excused. When you reach out ...
Showing: 221 - 240 of 545 results