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  3. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...This post was contributed by Matteo Tardelli, a postdoctoral scientist at Weill Cornell Medicine. Discussing...outside of the lab meeting setting. Check out the blog post “Fostering Communication in Remote Collaborations...blogger Matteo Tardelli! Matteo Tardelli, PhD is Postdoctoral Scientist at Weill Cornell Medicine in NYC and...

  4. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...This post was contributed by Deborah Sweet, Vice President of Editorial at Cell Press. Almost everyone...research more reproducible Read all of our blog posts on scientific publishing  ...enterprise will benefit. We’ll have fewer students and postdocs sitting frustratedly in reproducibility jail and... PhD at the MRC LMB in Cambridge UK and then a postdoc at The Scripps Research Institute in La Jolla, ..., California. She joined Elsevier after her postdoctoral studies, and has held various editorial roles...

  5. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...This post was contributed by guest writer Pamela J. Hines PhD, Senior Editor at Science Magazine. Although...Out Our Career Resources Subscribe to Career Blog Posts Resources at Addgene.org Find Job Openings at ...

  7. Science Career Options

    Type
    Blog Post
    ...career you actually want, you can feel lost. Use this post to explore the wide range of careers available to...sending out applications. Check out our other career posts for more specifics on some careers and to find advice...

  8. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique...thymine (T) to hybridize and form hydrogen bonds. This post focuses on "sticky end" TOPO (also called TOPO-TA...

  9. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’... detailed investigations into the release of DNA post-cut. Using substrate DNA labeled on both sides of... optional donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating...

  11. 2022 Depositor Week

    Type
    Blog Post
    ... our Depositor Week schedule:  Monday: Thank you post (this is it!)  Tuesday: Stats roundup on Twitter...

  12. Lentiviral Vector Guide

    Type
    Guide
    ... sequence. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the...

  14. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... covered five model organisms in a previous blog post, but here are five more model organisms that have... scientific community. (And, check out this blog post to see even more genetic systems for emerging model...organisms in the first Five Popular Model Organisms blog post Read more about plasmids in our Plasmids 101 blog...

  15. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of .... This is clearly the case when proteins undergo post-translational modification with fatty acyl chains...Introduction to GFP Read our Fluorescent Proteins Blog Posts Download the Fluorescent Proteins 101 eBook Resources...

  16. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...obstruct binding sites or motifs that are necessary for post-translational modifications. Despite these potential...Addgene blog Read our fluorescent proteins blog posts Download the fluorescent proteins 101 eBook Read...

  17. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...again, this post has a lot of acronyms, so we've included a glossary at the end of this post! Figure...In our last post, we talked about the first base transversion editors: CGBEs, or C → G Base Editors. ...

  18. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...right time. Like many of the posts in our Transferable Skills series, this post is not meant to be a comprehensive...details and vocabulary for this skill. What this post may do is help you realize whether or not you have... a conference poster session, you don’t necessarily want everyone who visits your poster to agree with...

  19. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...array of different techniques and protocols. In this post we will discuss the natural role of biotin, biotinylation...ligase (BPL) is responsible for this highly specific post-translational protein modification. Most organisms.... E. “The enzymatic biotinylation of proteins: A post-translational modification of exceptional specificity...

  20. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...cloning we once relied exclusively on. In this blog post, I will go over some advantages, disadvantages, ...Independent Cloning (SLIC) in a previous Plasmids 101 post. Although SLIC may be more cost effective, Gibson...the Addgene Blog Browse All of Our Plasmids 101 Posts Read About Other Cloning Techniques Additional ...

  22. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...to improve processes in the Addgene lab. In this post, I’ll tell you a little bit about what I learned... got to summarize my experience in my first blog post! I highly recommend doing the same if you ever go...

  23. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... smear across your FSH vs reporter plot. In this post, I’ll walk you through how to interpret the antibody...  Note that for the flow plots discussed in this post, voltage has been adjusted appropriately and compensation...

  24. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...hundred trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg...new tool, go forth and trace some neurons!  This post was originally written by Leila Haery in 2017 and...

  25. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...This post was contributed by Mary Tamer from BioBuilder, an innovative nonprofit bringing the study of...Optogentics for E. coli Browse All Synthetic Biology Blog Posts Resources on Addgene.org Guide to SynBio Assembly...chapter and an illustrated, downloadable classroom poster — with both of these resources road tested over...

  26. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...This post was contributed by Ignacio Amigo and María Hernández Sánchez from Crowdfight. In March 2020... resources on Addgene's blog Check out our blog post about remote collaborations Read our Transferable...science. Others are experienced researchers, such as postdocs and principal investigators. They also come from...IgnacioAmigoH     María Hernández-Sánchez is a postdoctoral researcher at Instituto de Investigación Biomédica...

  27. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    .... To dive deeper into this field read our recent post “Cancer and the Immune System: Deciphering the Relationship...on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages Cancer and the...open-source! Impressions from the poster sessions During the poster session I got a chance to talk with...tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited at Addgene...

  29. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...questions. Starting at the Broad Institute For his post-doc, Doench worked on using RNAi screens to learn...others at the Broad Institute here Read other blog posts about CRISPR Learn more about the Broad Institute...

  30. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...This post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes...F1000Research uses a novel form of peer review that occurs post-publication and is completely open; the reviewer...
Showing: 331 - 360 of 782 results