We narrowed to 808 results for: Eng

...experiments, and prepared your reagents. You’re going to engineer the best thing since CRISPR; you are ready to ...technologies currently available, you now face the challenge of trying to figure out what system might work...

...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...the tag for each insertion. Also, it remains challenging to screen for the genetically modified strains...

...daily, yet obstacles remain. One of the biggest challenges facing researchers today is the complexity of... of the latest resources? In response to this challenge, Addgene has launched an initiative to curate ...

...major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh ...) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated this system...

... peripheral nervous system presents a special challenge to scientists. What works well in one cell or ...vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors. I wanted...

...from very high cell density suspension. Biotechnol Bioeng. 2021 Sep;118(9):3499-3510. doi: 10.1002/bit.27776...Schöneich C, Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes...

...viruses, with a linear genome between 26–45 kb in length. Human adenovirus type 5 (hAdV-C5) can be considered... like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While the pre-existing...

...transcriptase (RT) is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of...2008). Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 DNA Synthesis...

...). Additional resources on the Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24...pSiM24 for simplifying plant genetic engineering Using arabidopsis in the classroom Resources on Addgene.org...

...bypassing these issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point mutations...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex." Nature517.7536 (2015): ...

...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...scientists of all types to stay up to date and engaged with new advances in published science. They also...

...negative sense RNA strand approximately 19 kb in length. It contains short promoter sequences at its 3’...replication and transcription, as it would for a full-length viral genome (Figure 2). While it is technically...

...Cpf1-mediated cleavage are 42-44 nucleotides in length, about the same size as Cas9’s, but with the direct...Figure from Kleinstiver et al., 2016. Cpf1’s strength may lie in its low off-target editing rates, determined...

...repair (HDR) (Glasser et al., 2016). For TREE they engineered a BFP variant that undergoes conversion to GFP...TLDR: TREE from the Wang and Brafman lab uses an engineered BFP variant that converts to GFP after a base...

...distinguish between Zika and a closely-related virus, Dengue from multiple sample sources. SHERLOCK could also...://doi.org/10.1126/science.aaf5573 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao...

.... During a transient leaf transformation, the engineered Agrobacterium strain is brought into contact ..., 2006. 225-229. PubMed PMID: 16739580. Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based...

...on-site, but now we were presented with a new challenge: automating the bioinformatics steps needed to... reviewing and testing several platforms for engineering pipelines, we selected Nextflow, a workflow management...

...from flow cytometry experiments can be a little challenging to understand if you’ve never done any flow. ... showing frequency of events on the Y axis and strength of signal read on the X axis may be presented....

... less time consuming and/or less technically challenging. Alongside there is a second-line team that takes...are just so many things which can go wrong in a lengthy protocol. And customers may tell all sorts of details...

...Central PMCID: PMC384129. Aizenman, Einat, Hanna Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal...PMID: 12718874. Yang, Junjie, Weihong Jiang, and Sheng Yang. "mazF as a counter-selectable marker for unmarked...

...Addgene blog Learn about what went into our search engine update Find all Addgene news Resources on Addgene.org... Sophia K. Cheng...

...counter-intuitive, but I find office work more mentally challenging than lab work at times.” Sometimes a desk job...to non-scientists can be a fun and gratifying challenge. You’re also getting the opportunity to learn ...

...response to requirements that emerged from intensive engagement with a wide range of people at the University... The two guiding principles emerging from the engagement with the Wisconsin research community were to...

...to the right - the Reddit community is active, engaged, and has great questions! 2. The r/Science subreddit...Getting on a good science career path can be challenging these days, but for many of us, scientist is ...

...multiple genes by CRISPR/Cas-mediated genome engineering." Cell 153.4 (2013): 910-918. PubMed PMID: 23643243...Addgene Blog Get the Basics of Mouse Genome Engineering with CRISPR Validate Your Genome Edit Learn about...

... cytoplasm. Generally, FPs evolved in or were engineered for use in cytoplasmic environments. However,...problems of popular fluorescent proteins and were re-engineered by mutating cysteine residues and N-glycosylation...

...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...recombinant proteins are typically expressed in E. coli engineered to accomodate high protein loads using inducible...

...linking the quantity of absorbed light, at a given wavelength, to the concentration of fluorophore in solution...method, where the donor is excited by a specific wavelength of light and the signal is collected by using...

...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...Mali, Prashant, et al. "RNA-guided human genome engineering via Cas9." Science 339.6121 (2013): 823-826. ...

...maximum length of codons that can be mutated? 88 codons, or 264 basepairs, was the maximum length tested...

... perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive research...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs...

...sites? Never fear! You can introduce a silent, passenger edit to serve as a proxy for your edit of interest... a version of Cas9 that has been specifically engineered for reduced off target editing, such as SpCas9...

...photoreceptors (Oliinyk et al., 2019). They were engineered to incorporate BV. Their advantages are their...Verkhusha, V.V. Near-infrared fluorescent proteins engineered from bacterial phytochromes. Curr Opin Chem Biol...

...(see Figure 2). H describes the maximum signal strength, while W results from the time that the cell spent...increase the electric current and thereby the signal strength. So, we need to adjust the voltage in the cytometer...

...used here could be an attractive strategy for engineering other enzymes. Conclusion We hope this post has...precise control over vascular endothelial barrier strength. eLife, 12, RP84364. https://doi.org/10.7554/eLife...

...called Drosha to pre-miRNA. miRNA plasmids are engineered to skip this first pri-miRNA step. These RNA ...1472-6750-6-1 O’Brien, J., Hayder, H., Zayed, Y., & Peng, C. (2018). Overview of MicroRNA biogenesis, mechanisms...

...validation PCR followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction... absence) of that particular site with fragment length polymorphism (RFLP) analysis. In this process, ...

...First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity, caused partially...Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire...

..., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L..... E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric...

... you? Maybe you like things a bit hectic and challenging and this drives your best work. Or is it teamwork... comparison can be very helpful in uncovering strengths and weaknesses of the options. Use this blog as...

...important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments...

... signal, so you’ll need a range of expression strengths to be confident in your data. This is a good option...9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL, Cragg MS,...

...Sleeping Beauty, piggyBac and Tol2 for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084...about the INTEGRATE system for bacterial genome engineering  Read about the Sleeping Beauty DNA transposon...

...are currently about 5.5 million articles in the English version of Wikipedia alone. Humans have been forced...a team is a really good way of creating and strengthening those social bonds. So never mind how much better...

...Creating epitope-tagged clonal cell lines remains a challenge as it relies on inefficient homology-directed ...representing a protein super family). To overcome these challenges, we set out to develop a hassle-free, highly ...

...our pipeline. In it, they break down the unique challenges of our platform and how we were able to address...

...make-over Get a how-to guide on the new Addgene search engine ...

...the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements and enhanced...

... X, formerly Twitter. If you are interested in engaging with us on social media, you can find us on Bluesky...

...the 1st day of CRISPR, Addgene gave to me: An engineered prime editor with enhanced editing efficiency...