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We narrowed to 862 results for: LIC

Showing: 551 - 600 of 862 results

  3. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ... mutations in single stranded DNA undergoing replication.   DNase on the other hand can digest and degrade...directly use your DNA preparation in any downstream application involving enzymatic reactions, a valid alternative...

  4. Fostering communication in remote collaborations

    Type
    Blog Post
    ...meetings as opposed to phone calls most closely replicates how you’d interact with team members in person...access data and drafts of manuscripts and grant applications in real-time so they can provide input at the...

  5. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...parasitization of cereal crops. As a result, agricultural applications of these novel biosensors could have a major...CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  CRISPR-Act3.0, ...

  7. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable...

  8. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...is resistant to specific compounds, links to publication references, and cross-references with cell line...manually going through the pdfs of the 26,000 publications cited. If the information seems incomplete or...

  9. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make...

  10. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ... perspective – a plasmid-containing cell must replicate the plasmid in addition to its own chromosomal...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...

  13. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR...

  15. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...these tools, which aren’t always easy to find in publications, so this is a much-needed community resource...exactly which strain you used so if they wish to replicate your experiment, they know exactly where to get...

  17. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in...Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue...

  18. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...preparations have worked well in my lab, including in applications where we have delivered them in vivo to the ...distributing new types of materials. At the time of publication we’ve already fulfilled more than 140 orders ...

  19. Plasmid Cloning by PCR

    Type
    Blog Post
    ...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly...

  20. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...lymphocytes, too, making them a versatile tool for application in both neuroscience and immunology. Don’t be...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...

  21. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...you will have. When possible, use low-bind or siliconized tubes to store samples as it is more difficult...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ...

  23. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ... we found in our map have an impact on virus replication. While we and our collaborators are starting ...coronavirus, one FedEx package at a time Since the publication of our paper, our group has been inundated with...

  25. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...

  27. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve...of Toronto. He is an author of 20+ scientific publications and patents.   ...

  28. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ...

  31. Antibodies 101: ChIP

    Type
    Blog Post
    ...next-gen sequencing. ChIP is an incredibly useful application for exploring these interactions, and can give... upfront investment than many other antibody applications, this investment can well be worth it once you...

  33. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics, in this...Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct...

  34. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color...

  35. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ... PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers...

  38. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ... this stage and that can be used to simulate in silico cloning. This will definitely help in understanding...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

  39. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...modular suite of plasmids (pGT) with various replicative origins that can be used for broad or narrow ...Central PMCID: PMC5963681.  Meyer, Richard. "Replication and conjugative mobilization of broad host-range...

  43. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...IgG2 or IgG4. This can be important for some applications. Assay use While secondary antibodies are useful..., the flexibility of their use varies across applications. Western blots, for instance, almost always ...

  47. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ... as secondary antibodies, have much broader applicability. It is fairly easy to find these in a Fab fragment...fragments described above can be used for similar applications and have similar advantages, though with some...

  48. Antibodies 101: Conjugation

    Type
    Blog Post
    ...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007...

  49. All in a Twist: dsRNA

    Type
    Blog Post
    ...generate dsRNA as part of their infection and/or replication process within the host. dsRNA viruses are inherently...generate dsRNA intermediates as part of the viral replication process. dsDNA viruses yield dsRNA through a ...

  50. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...can fail but, in our current setup, there are no explicit instructions on what to do when something fails... We can set ourselves up for success by giving explicit instructions to lab members through the software...
Showing: 551 - 600 of 862 results