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We narrowed to 507 results for: RAP

Showing: 221 - 240 of 507 results
  1. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post
    ... mixture on solid media. The colonies are then scraped off the plates and pelleted for DNA extraction....amplification looks like, but by looking at these kinds of graphs, making sure few/no plasmids have dropped out, ...
  2. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...highly cited, we went to one of the largest bibliographic databases available, Web of Science. For each... publication, Web of Science provides basic bibliographic information and uses its vast network of articles...
  3. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...figure below). Subsequent exposure to red light rapidly deactivates the system. Voigt’s lab used this system...GFP reporter. Subsequent exposure to red light rapidly deactivates the system. Version 1 of this far-red...
  4. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...throughout the body. Because calcium levels change rapidly during neuronal activity, scientists can use GECIs...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...
  5. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...it, marking it as GFP+. Quantification for such graphs could simply be the percentage of the total cells...background in protein mutagenesis and cancer immunotherapies. She is currently a Research Assistant in Arlene...
  6. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post
    ...region of the 3’ LTR and are primarily used in gene therapy research to reduce promoter interference and/or...References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses for efficient gene delivery...
  7. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate Cas9 cutting sites and... GEmCherry2 reporter systems provide efficient, rapid, and real-time read-outs of Cas9/gRNA efficiency...
  8. Viral Vectors 101: Types of viruses

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    Blog Post
    ...AAV has many excellent features for human gene therapy and is currently being used in clinical settings...Potential Applications for Gene and Cell-Based Therapies in the New Era of Personalized Medicine. Genes...
  9. A Deep Dive into BioBuilder

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    Blog Post
    ...students to explore how engineering fields rely on rapid prototyping to develop and test hypotheses. Students...Students then use cellular extracts as a means to rapidly prototype DNA circuits and logic gates. This BioBuilder-Caltech...
  10. General Transfection

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    Protocol
    ...-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only a few drops...
  11. Immunocytochemistry

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    Protocol
    ...min at RT in the dark. Pro-Tip The plate can be wrapped in foil to block light. Remove the secondary antibody...
  12. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post
    ...could greatly enhance our ability towards CTD therapeutic development,” says Iyer. Since the RESOLUTE plasmid...says Mellett. This would help the lab identify therapeutic targets in the future. Congratulations Mark Mellett...
  13. To Each HIS Own

    Type
    Blog Post
    ... isolated from a complex mix using affinity chromatography against an amino acid sequence, or tag, on ...Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient purification of native histidine-tagged...
  14. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ... discovery and the development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al...Studying clonal dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine...
  15. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... basic biological research as well as clinical therapy. These biological tools are useful because they...involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common...
Showing: 221 - 240 of 507 results