We narrowed to 497 results for: RAP

1. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   ...each genotype with vulnerabilities) to design a therapeutic strategy that works. But, those darn cancers ...Library for Lineage Tracing Learn About Cancer Immunotherapies   Resources on Addgene.org Fine More Kits...

2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   ...fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate Cas9 cutting sites and... GEmCherry2 reporter systems provide efficient, rapid, and real-time read-outs of Cas9/gRNA efficiency...

3. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   ...it, marking it as GFP+. Quantification for such graphs could simply be the percentage of the total cells...background in protein mutagenesis and cancer immunotherapies. She is currently a Research Assistant in Arlene...

4. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   ...region of the 3’ LTR and are primarily used in gene therapy research to reduce promoter interference and/or...References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses for efficient gene delivery...

5. Viral Vectors 101: Types of viruses

   Type

   Blog Post

   ...AAV has many excellent features for human gene therapy and is currently being used in clinical settings...Potential Applications for Gene and Cell-Based Therapies in the New Era of Personalized Medicine. Genes...

6. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   ...talk at the Cell Symposia: Gene- and Cell-based Therapies: CRISPR, Stem Cells, and Beyond meeting earlier...and faster ABEs (Fig. 2). These systems allow for rapid continuous protein evolution through many rounds...

7. Protocol - Over-Agar Antibiotic Plating

   Type

   Protocol

   ...after Over-Agar Plating of Carbenicillin. The above graph displays the stock concentration of Carbenicillin...

8. Supporting Rare Disease Research with the 2020 BeHEARD Awards

   Type

   Blog Post

   ...could greatly enhance our ability towards CTD therapeutic development,” says Iyer. Since the RESOLUTE plasmid...says Mellett. This would help the lab identify therapeutic targets in the future. Congratulations Mark Mellett...

9. To Each HIS Own

   Type

   Blog Post

   ... isolated from a complex mix using affinity chromatography against an amino acid sequence, or tag, on ...Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient purification of native histidine-tagged...

10. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    ...what if we had a simple tool that allowed us to rapidly decode the complexity? The CombiGEM-CRISPR technology...for genome editing in large-scale has led to the rapid generation of genome-wide gRNA libraries that knock...

11. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    ... discovery and the development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al...Studying clonal dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine...

12. Protocol - How to Perform Sequence Analysis

    Type

    Protocol

    ...sequencing results as a trace file (.ab1) which graphically depicts the sequence as a series of colored peaks...

13. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    ...section, contact information, and potentially a photograph of yourself. A photo can be very useful in the.... Shapes. In this type of poster, written and graphical information are inserted into larger circles or...

14. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    ...orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo AG, Gautier... for chromophore maturation, splitFAST displays rapid and reversible complementation. TagBiFC (split HaloTag...

15. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    ... basic biological research as well as clinical therapy. These biological tools are useful because they...involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common...

16. Engaging with science and society at pgEd

    Type

    Blog Post

    ...these tough issues is essential for building a rapport with these communities, which is in turn crucial...plans, or information briefs for policymakers. The rapidly changing landscape of genetics and our consistent...

17. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    ...p24 levels in a viral prep is straightforward and rapid as there are several commercially available p24 ...predicting gene transfer events." Genetic vaccines and therapy 2.1 (2004): 1. PubMed PMID: 15291957. PubMed Central...

18. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    ...to 100. This transformation (illustrated in the graph below) is performed because weak gRNAs (CFD < 20...weight (which ranges between 0 and 1) (shown in the graph below) so that it can be used in the rank equation...

19. Plasmids 101: Degron Tags

    Type

    Blog Post

    ... been removed. The degradation and reversal are rapid compared to other systems because the system modulates...Cleveland DW. Inducible, reversible system for the rapid and complete degradation of proteins in mammalian...

20. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    ... virus may acquire mutations to escape these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer...approach can augment viral surveillance by enabling rapid assessment of antigenic drift.    Starr et al Cell...