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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 249 results for: TIR

Showing: 221 - 240 of 249 results

  5. Deep Dive: qPCR

    Type
    Blog Post
    ... you to measure the intensity of signal over an entire cycle. Since signals are produced by the presence...

  6. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...identified as non-essential. The knockouts replace entire protein-coding regions (excluding start and stop...

  8. AAV Production in HEK293 Cells

    Type
    Protocol
    ...needed. Add stir bar and stir slowly at 4 °C for 1 h, then keep at 4 °C for 3 h without stirring to allow...controller Hazardous waste container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents...PEI powder into 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...deionized water and adjust to a final volume of 1000 mL. Stir at RT until fully dissolved. Pro-Tip This step is... challenging due to the high viscosity of PEG. Stirring under medium heat will promote faster dissolution... to ~7.4. Autoclave or sterile filter. Pro-Tip Stirring during the cooling period is recommended or the...proceed overnight at 4 °C if needed. Transfer the entire sample to 3 x 500 mL conical bottles and centrifuge...

  9. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved...platform set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant...presence of particles still in solution. Continue to stir until all particles have dissolved. This may take...

  10. CRISPR Guide

    Type
    Guide
    ...generate large-scale genetic changes, including moving entire genes to different locations, by combining other...you’ll need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then ...

  11. Kit Free RNA Extraction

    Type
    Protocol
    ...wash and add RNase-free water or TE as soon as the entire tube is dried but while the white pellet is still...Pro-Tip To avoid multiple freeze-thaw cycles of your entire RNA sample, consider making smaller aliquots of...

  12. Lentivirus Production

    Type
    Protocol
    ...controller Hazardous waste container pH meter Stir plate Magnetic Stir Bar Reagents DMEM high glucose, Corning... of powder in 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...

  13. Cloning

    Type
    Guide
    ...advantage of type IIS systems are two-fold. First, the entire cloning step (digestion and ligation) can be carried...

  14. Chemogenetics Guide

    Type
    Guide
    ... DREADDs in experiments in this species may be entirely due to back-metabolism of CNO to clozapine, demonstrating...

  15. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...portion of the insert, but we do not sequence the entire plasmid. Addgene strongly recommends that you...Make sure the primer only anneals once within the entire construct. Is the primer an appropriate distance...

  16. Lentiviral Vector Guide

    Type
    Guide
    ...and wild-type LTRs Packaging plasmid — contains entire viral genome (packaging, regulatory, and accessory...

  17. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying from a plasmid or...plenty of starting material. We recommend using your entire PCR reaction and 1μg of recipient plasmid. It is...

  20. General Transfection

    Type
    Protocol
    ...of powder into 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...
Showing: 221 - 240 of 249 results