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...Shakespeare for the first time. The discovery and application of CRISPR-Cas9 kicked off over a decade of frenzied...to subtype to variant. Despite the seemingly complicated steps to arrive at this hierarchy, the main aspects... Class to type to subtype to variant (shown in italics). Created with BioRender.com.   Class 1 versus...action power of Cas3. Cas3 functions as both a helicase and a nuclease, unwinding and cutting up DNA after...nuclease effector. However, IV-C does in fact have a helicase domain due to a gene that resembles cas10. All... use in mammalian systems, with the landmark publication in 2013. Since that point, it has become the ...mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Molecular Cell, 84(3), 463-475.e5. https://...

...the best, or even which one will work for your application? Like many scientists, I have spent hours sifting...produced from a new set of animals), and wrong application (different experiments/conditions can change ...differently over time), compatible with the desired applications (such as immunohistochemistry (IHC) or western... members (like me!) are dedicated to creating a public and accessible database for low-cost, high-quality...positive, it will be released through the website for public reference. The Gift of Giving As an open-source...for goldilocks antibodies and, alongside that, a publicly available 2D/3D mouse atlas from antibodies that...resolving the antibody crisis. The mAb3D Atlas is a public service to the science community, and I am excited...

...scientists can mutate the transcription start site, splice sites, exons, and/or promoters to knock out lncRNA... and Bester 2021; Zibitt et al. 2021). Mutating splice sites is particularly effective as it can result...Transcription start site (TSS) deletion. (B) Mutations of splice sites. (C) Removal of an exon or a large genomic...it comes to targeting precision and practical application. If the CRISPR/Cas9 system is not effective for...lncRNAs with high specificity, particularly in applications requiring smaller delivery systems (Zibitt et...noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol. Published online November...expression by lncRNA promoters, transcription, and splicing. Nature. 2016;539(7629):452. doi:10.1038/nature20149...

..., its dimeric nature can interfere with some applications. Several labs have been working to produce monomeric...Image reused from Ando et al. 2023, under CC-BY license.   The Miyawaki lab generated a series of ... for molecular fusion and membrane-targeting applications. Nature Methods, 1–9. https://doi.org/10.1038...Chuapoco, Flytzanis, Goeden, et al. 2023, under CC-BY license.   Find the AAV.CAP-Mac plasmid here!   Chuapoco...rats. Figure from Chen et al (2023) under CC-BY license.   Find AAV1-X1 plasmid here! Chen, X., et ...proven itself a good option for essentially all applications. Addgene’s plasmid collection includes over ...clone (M2) has been widely used in a variety of applications. Find anti-DYKDDDDK recombinant antibodies here...

...Co-Ordinating Centre for Public Engagement (NCCPE) provides on-site courses in public engagement for institutions...tips on using social media as a scientist here. 2) Public engagement - Participate in science festivals and... and outreach If you are comfortable talking in public, volunteer at science festivals. Or engage with...performance. When your institute opens its doors to the public, for example at an anniversary or for Girls’ Day...for 10 busy weeks at media outlets like National Public Radio, WIRED, Scientific American or National Geographic...projects Lastly, if you'd like to directly involve the public in your research projects, you can set up a citizen...

...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...

... right AAV for your system or to successfully replicate published works. Of particular importance to these... focus on getting this information out into the public where it can be utilized by others. Addgene’s AAV...citable DOIs through DataCite before being made publicly available through the Data Hub. These minimum ...performance. Yet, most of the technical data needed to replicate and build on others’ results is available not ...information provided in openly accessible forms like publications. Why not get this data out there?  The Addgene...your data - negative AND positive - out into the public eye where it can benefit the community. We are ...Freedom: The Expansion of Data Sharing in Research Publications...

...valuable to scientists, there are some potential complications that must be considered when using this method...alternative approach, which avoids many of these complications, is to directly deliver a ribonucleoprotein ...used for most of the current genome engineering applications of CRISPR including: generating single or multi-gene...pathways. This makes Cas9 RNPs useful for CRISPR applications where limited expression of Cas9 is required...protocols outlining these steps have been made publicly available by both the Jacob Corn and Alex Schier... of eventually using this method for in vivo applications. This technique utilizes Cas9 proteins harboring...CRISPR experiments can reduce many potential complications, there are additional factors to take into account...

...strong immunogenic response, useful for clinical applications as vaccines. Selecting a Viral Vector Type Each...been carefully engineered for safety and are replication-incompetent. Specific genes have been mutated...mutated or deleted to prevent uncontrolled viral replication, and the required genes are divided in the multi-plasmid...

...to dramatically increase the number of CRISPR applications. The first non-Cas9 CRISPR protein adapted for...doi.org/10.1038/nbt.3609 Find plasmids from this publication at Addgene. Kim E, Koo T, Park SW, Kim D, Kim...10.1038/ncomms14500 Find plasmids from this publication at Addgene. Kleinstiver BP, Prew MS, Tsai SQ...10.1038/nature14592 Find plasmids from this publication at Addgene. Kleinstiver BP, Tsai SQ, Prew MS...doi.org/10.1038/nbt.3620 Find plasmids from this publication at Addgene. Hu JH, Miller SM, Geurts MH, Tang...10.1038/nature14299 Find plasmids from this publication at Addgene. Walton RT, Christie KA, Whittaker.../j.cell.2015.09.038 Find plasmids from this publication at Addgene. Additional Resources on the Addgene...

...performs well in many different antibody-based applications.  IPI’s recombinant version is a fusion of mouse... reused from Anderson et al., 2024 under CC BY license.    Find Anti-V5 [IPI-SV5-Pk1] Antibody here...adapted from Kroupova et al., 2024 under CC BY license. This construct can be easily expressed and...Image reused from Cao et al., 2024 under CC-BY license.  These optimized fluorescent proteins will...cellular processes in C. elegans: mScarlet3 for applications needing intense brightness (such as low-expression...first use of CRISPR/Cas9 in M. abscessus. The simplicity and efficiency of this dual-plasmid approach ...Image reused from Neo et al., 2024 under CC-BY license.   Find plasmids for CRISPR/Cas9 in M. abscessus...

...activity of CcdB. Applications of ccdB in plasmid cloning Perhaps the most widely used application of CcdB is...used as biological tools for a wide range of applications in molecular biology or in vivo studies. Let...types of plasmid addiction systems and their applications. The discovery of toxin-antitoxin systems Toxin-antitoxin...CcdA (antitoxin) where CcdB inferes with DNA replication by binding the GyrA subunit of DNA gyrase and...construct. Other toxin-antitoxin systems and their applications MazE/MazF Aside from CcdB/CcdA, there are many...Kulakauskas, Saulius, Arvydas Lubys, and S. Dusko Ehrlich. "DNA restriction-modification systems mediate ...

... citations for recent articles. Of course, a publication in Science or Nature is always nice, but in the... lesser-known journal as it is in a top-tier publication. Accessibility is surely also behind the increase...increase in citations for older publications. (As an interesting aside, Nature recently reported that the...in a solution.) But are citations and journal publications really the best way to measure impact? As that...and what it’s made of. Increasingly, journal publications alone aren’t enough. Scientists are sharing ...consider and quantify the impact of scientific publications. Ventures like Impact Story seek to find ways...

...decades. Widely used across various research applications, they have become indispensable tools for manipulating...-lasting changes in cells. However, for some applications, integration might be considered a double-edged... is distributed across multiple plasmids. For simplicity, we’ll use the second-generation system as an...dividing cells because they lack an origin of replication (Banasik and McCray, 2010). However, IDLV genomes... certain types of clinical or basic research applications. Considerations for IDLV use If you’re reading...Integrase-defective lentiviral vectors: progress and applications. Gene Therapy, 17(2), 150–157. https://doi.org...lentiviral vector: prospects for safe clinical applications. PeerJ, 10, e13704. https://doi.org/10.7717/...

...current CRISPR research focuses on its potential applications for human medicine (Waltz, 2016), the potential...selected the correct CRISPR components for your application, it is time to deliver these components to your...detergent, Polyethylene Glycol (PEG), although the application of this approach is limited to protoplast cells...find many plasmids for a wide variety of CRISPR applications in plants available through Addgene. In addition..., we highly recommend reading the associated publications or protocols to get the most out of the plasmid...N (2016) The CRISPR/Cas Genome-Editing Tool: Application in Improvement of Crops. Front Plant Sci 7: ....10.1073/pnas.1818290116 Zhu H, Li C, Gao C (2020) Applications of CRISPR–Cas in agriculture and plant biotechnology...

... February, the Office of Science and Technology Policy issued a memorandum calling on Federal agencies...in annual R&D expenditures to support increased public access to the results of federally-funded research...an organization called Free the Data launched a public database to gather the results of Myriad's genetic...support for open access to peer-reviewed journal publications, although many don’t necessarily publish in ...gave preference to journal access granted via a public repository or authors’ own web sites. 9)      The...to share science. Image credit: Judy Breck, via flickr  ...

...Institute for Cell Science has produced the first publicly available collection (Link opens in a new window...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ...: 28814507 (Link opens in a new window) More publications from the Allen Institute for Cell Science (Link...

...Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS...tet-systems-legacy-products/tet-on-and-tet-off-2nd-generation Mullick, A., Xu, Y., Warren, R. et al. The cumate gene-... PMCID: PMC2942869. Find plasmids from this publication at Addgene. del Valle Rodriguez, Alberto, Dominic... PMCID: PMC2883883. Find plasmids from this publication at Addgene. Wei, Xing, Christopher J. Potter... PMCID: PMC3846601. Find plasmids from this publication at Addgene. Riabinina, et al. “Improved and ... PMCID: PMC4344399. Find plasmids from this publication at Addgene. Riabinina O, Potter CJ. The Q-System...

...Prepare by going sleuthing on the lab website, publication records, LinkedIn, ResearchGate or Twitter. Be...the management style? How does the PI deal with conflicts in the lab? Do they motivate with the appropriate...the appropriate first authorship position on a publication of her project and made another lab member first...professional status – including belittling opinions, public professional humiliation, accusations regarding... Is the work (topic and/or technical aspects) applicable to industry if this path interests you  (e.g....connections such as serving on Boards? Does the publication record show evidence of industry collaboration...out PI intervenes if there are interpersonal conflicts PI has no idea who is dating whom Expanding...

... ReFigure. Reading and exploration including replications and experiments resulting in “negative data”...small selection of data found in full-length publications. As early career researchers, we wondered how...performance as in this antibody ReFigure Analyzing replications as in this ReFigure about whether Natronobacterium...reagent performs well, or you might publish a replication experiment that you conducted as part of setting...papers as they are not included in traditional publications. By submitting your data to a repository (Figshare...-registration, preprints, data, software, micropublications) in grant proposals. ReFigures are one such...