We narrowed to 517 results for: pes
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TypeBlog Post...is induced by various types of interactions with their environment. These types of interactions to a large...growth properties, migration abilities, or other phenotypes that can be efficiently measured at the single...
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A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post.... There are numerous variants for the different types of opsins available and their differences and uses...See the Deisseroth lab homepage for details about types of constructs available, instructions for obtaining...cell-type specific opsin expression. However, the types of opsins you are able to use are obviously limited... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...different scoring algorithms and parameters. This tapestry of ideas is what makes choosing the right tool...input in order to generate results. While different types of input are more user-friendly than others, this...less concern than data output as different input types can be readily converted. Examples: “organism”,... -
Delivery Methods for Generating iPSCs
TypeBlog Post...and mumps. Sendai transduces a wide range of cell types and replicates in the cytoplasm independent of the...and protein delivery are better suited for these types of studies. What cells are reprogrammed will also...also affect the outcome, since not all cell types are easily attainable, or easy to reprogram. Basically... -
27 Hot Plasmids from 2016
TypeBlog Post...genome for genes that regulate a wide variety of phenotypes, including but not limited to cell growth and...chosen to represent the primary splice variant in 38 types of human cancer. Many of the transcripts are not...create protein expression constructs fused with epitopes or fluorophores, generate expression vectors with...investigating hard-to-study genes in hard-to-study cell types. Natsume T, et al. Cell Rep. 2016. PubMed PMID:... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post... proteins coat the virus and help determine the types of cells that it can infect. The VSV-G envelope ...it enables lentiviruses to infect many different types of cells. This means researchers who use VSV-G can... -
AAV Packaged on Request is Here!
TypeBlog Post... be packaged in your choice of five different serotypes. We are so excited for all the ways this much-requested...Packaged on Request banner. We currently offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV Retrograde (AAVrg... -
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog Post... joined the Addgene Board of Directors where he hopes to help keep us focused on our core mission to promote...using a decellurized scaffold as a base. Darrell hopes that further advancement of this technique will ... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post... she is currently working on a version that she hopes will change the intracellular localization of RNA...Her lab is currently investigating the issue and hopes to have a better understanding of the phenomenon... -
Reflections and Looking Ahead
TypeBlog Post...collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub reports detailing antibody...can now be packaged on demand in five distinct serotypes, offering you unparalleled flexibility. But this... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post...genes have different functions in different cell types, at different times, and in combination with different...L-CHIME Knocks out a single gene in defined cell types (lineage-based) pXPR_070 Violet-excited GFP ... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post...perturbations in several genes spanning 33 cancer types. The types of mutations they identified included gene... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog Post...repository and their choice of five different serotypes. Figure 1: The process of requesting...service. At launch, we will offer five different serotypes for packaging: AAV1, AAV2, AAV5, AAV8, or AAV ... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post... continue to improve plant nutrition, yield, and pest resistance. Much of plant genetic engineering revolves...DNA yield and transformation efficiency in both types of bacteria. They also replaced the promoter regulating... -
Year of the Camelid: Antibody Style
TypeBlog Post...access unique epitopes with high affinity, due to a greater ability to target concave epitopes Suitability... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...VP3). There are eleven naturally occurring AAV serotypes that have thus far been identified: AAV1-11. Nine... in the cap gene which differ between the AAV serotypes are responsible for serotype-specific differences... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...technology is versatile and can be applied across cell types and organisms by replacing the transcription factor... allow sparse labeling in a variety of fly cell types. Because the PhiC31 recombinase can work in other... -
Sensing Neuronal Dopamine
TypeBlog Post...and freely behaving mice to gather the following types of data: Report time-course and concentration of... and freely moving mice to gather the following types of data: Report endogenous DA release from a single... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...a marker for the mutation. When designing these types of experiments it is best practice not to use the...compatible! Check out this list of common E. coli genotypes and their natural resistances. Additional Resources... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...drum. They have a wide variety of possible genome types that can be either double stranded (ds) or single...them far safer to work with than virions. The four types of viral vectors (which many researchers will informally...