... knock-in efficiency across different stem cell types. This has facilitated easy generation of clonal ...choice for epitope tagging in diverse stem cell types. Hundreds of genes can be epitope-tagged in a couple...large protein cohorts in diverse mammalian cell types. A detailed step by step protocol for the csRNP ...

.... There are numerous variants for the different types of opsins available and their differences and uses...See the Deisseroth lab homepage for details about types of constructs available, instructions for obtaining...cell-type specific opsin expression. However, the types of opsins you are able to use are obviously limited...

...different scoring algorithms and parameters. This tapestry of ideas is what makes choosing the right tool...input in order to generate results. While different types of input are more user-friendly than others, this...less concern than data output as different input types can be readily converted.  Examples: “organism”,...

...and mumps. Sendai transduces a wide range of cell types and replicates in the cytoplasm independent of the...and protein delivery are better suited for these types of studies. What cells are reprogrammed will also...also affect the outcome, since not all cell types are easily attainable, or easy to reprogram. Basically...

...genome for genes that regulate a wide variety of phenotypes, including but not limited to cell growth and...chosen to represent the primary splice variant in 38 types of human cancer. Many of the transcripts are not...create protein expression constructs fused with epitopes or fluorophores, generate expression vectors with...investigating hard-to-study genes in hard-to-study cell types. Natsume T, et al. Cell Rep. 2016. PubMed PMID:...

... proteins coat the virus and help determine the types of cells that it can infect. The VSV-G envelope ...it enables lentiviruses to infect many different types of cells. This means researchers who use VSV-G can...

... be packaged in your choice of five different serotypes.   We are so excited for all the ways this much-requested...Packaged on Request banner.   We currently offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV Retrograde (AAVrg...

... joined the Addgene Board of Directors where he hopes to help keep us focused on our core mission to promote...using a decellurized scaffold as a base. Darrell hopes that further advancement of this technique will ...

... she is currently working on a version that she hopes will change the intracellular localization of RNA...Her lab is currently investigating the issue and hopes to have a better understanding of the phenomenon...

...collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub reports detailing antibody...can now be packaged on demand in five distinct serotypes, offering you unparalleled flexibility. But this...

...genes have different functions in different cell types, at different times, and in combination with different...L-CHIME Knocks out a single gene in defined cell types (lineage-based) pXPR_070 Violet-excited GFP ...

...perturbations in several genes spanning 33 cancer types. The types of mutations they identified included gene...

...repository and their choice of five different serotypes.   Figure 1: The process of requesting...service.  At launch, we will offer five different serotypes for packaging: AAV1, AAV2, AAV5, AAV8, or AAV ...

... continue to improve plant nutrition, yield, and pest resistance. Much of plant genetic engineering revolves...DNA yield and transformation efficiency in both types of bacteria. They also replaced the promoter regulating...

...access unique epitopes with high affinity, due to a greater ability to target concave epitopes Suitability...

...and freely behaving mice to gather the following types of data: Report time-course and concentration of... and freely moving mice to gather the following types of data: Report endogenous DA release from a single...

...technology is versatile and can be applied across cell types and organisms by replacing the transcription factor... allow sparse labeling in a variety of fly cell types. Because the PhiC31 recombinase can work in other...

...VP3). There are eleven naturally occurring AAV serotypes that have thus far been identified: AAV1-11. Nine... in the cap gene which differ between the AAV serotypes are responsible for serotype-specific differences...

...a marker for the mutation. When designing these types of experiments it is best practice not to use the...compatible! Check out this list of common E. coli genotypes and their natural resistances.  Additional Resources...

...antibodies within certain classification groups called isotypes.  Why does isotype matter? Isotype matters when...secondary. If you were to change one of the antibody’s isotypes, however, you could then use 2 different secondaries...