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  1. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...for packaging: AAV1, AAV2, AAV5, AAV8, or AAV Retrograde (AAVrg) capsids.  Please be aware that this ordering...plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013 and titers for AAV2 to be 7x1012...
  2. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...ahold of your destinies” and also suggested some concrete actions that can be taken now to solve some of...Disequilibrium, Disillusion and The Postdoc Dilemma” which pretty much said it all.  She cited the oft quoted statistic...
  3. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ...
  4. Antibodies 101: Chimeric Antibodies

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    Blog Post
    ...In most cases, the resulting chimeric antibody retains the binding specificity to the original target ...In addition, chimeric antibodies will inevitably retain some sequence from their parental species which...
  5. The Strength of Story Telling

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    Blog Post
    ...be the first author. When I raised this, he was pretty angry and started arguing with me. First I calmed...jumping backwards in time to explain something, rethink your structure. Develop your protagonist In most...
  6. The Importance of High Titer for AAV Transductions

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    Blog Post
    ...therefore higher titer will be required. For example, retrograde AAV gets transported up the cell’s axon. While...even larger area, thus necessitating even higher retrograde AAV titers for successful transduction. In another...
  7. The MTA at Addgene

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    Blog Post
    .... Over the last twenty years, we’ve developed a pretty smooth system for this process. We often get positive... sent the MTA to sign. Once they've signed and returned the MTA, Addgene can move forward with shipping...
  8. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...sequencing is successful, your NGS provider should return results in the form of many, many short DNA sequences...specific libraries, but any analysis script should return the same output: a spreadsheet of read counts. ...
  9. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...envelope protein for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids...as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates...
  10. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...plot. In this post, I’ll walk you through how to interpret the antibody readout on a flow plot.  Note that...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...
Showing: 221 - 240 of 587 results