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Showing: 221 - 240 of 552 results

  1. The Strength of Story Telling

    Type
    Blog Post
    ...be the first author. When I raised this, he was pretty angry and started arguing with me. First I calmed...jumping backwards in time to explain something, rethink your structure. Develop your protagonist In most...

  3. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...therefore higher titer will be required. For example, retrograde AAV gets transported up the cell’s axon. While...even larger area, thus necessitating even higher retrograde AAV titers for successful transduction. In another...

  4. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...sequencing is successful, your NGS provider should return results in the form of many, many short DNA sequences...specific libraries, but any analysis script should return the same output: a spreadsheet of read counts. ...

  5. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...Peak emission (nm) Size (kDa) Substrate Cofactors Secreted Luminous shrimp(Oplophorus gracilirostris) 455...cell membrane and deliver the substrate; however, secreted luciferase or the use of alternative substrate...

  7. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided on an application...attenuated, nef-deleted HIV-1 strains in vivo. Retrovirology. 2007 Sep 23;4:66. doi: 10.1186/1742-4690-4-...

  9. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...website resources that best match a user’s query, and return the matching resources in an order that makes sense...expose as filters and designed the search engine to return only the filters that are relevant to a specific...

  10. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ... and sequence displays are now much easier to interpret and analyze at a glance. For a quick look at just...“Analyze Sequence” button. The maps hosted here retain all the features shown in the static maps discussed...

  11. How Addgene Creates Content

    Type
    Blog Post
    ...quickly." Final design (right). Immediate and immense regret of the graphic designer not shown.   However... and knowledge changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid...

  12. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...envelope protein for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids...as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates...

  13. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...plan for a career outside academia (that would be pretty much all scientists), a LinkedIn profile is absolutely...rules of good resume writing. Include examples of concrete accomplishments in both the technical and “plus...

  14. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...activators or repressors. These fusion proteins retain the ability to recognize and bind to DNA; when ...occasions truncated gRNAs (≤15nt) have been found to retain some ability to induce Cas9 to make edits (AC, ...

  15. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...gender analysis and 2) provides case studies with concrete examples of how gender analysis leads to discovery...design. But eyes have been opened—and we cannot return to a world that ignores gender. Many thanks to...

  17. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...fiction story that doesn’t require any detailed retention of information or formulation of new knowledge...mental bandwidth.  Jargon Learning, using, and retaining new vocabulary takes time and effort (otherwise...

  18. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...demonstrate SEND as a modular system for packaging, secretion, and delivery of specific RNAs, such as Cre mRNA...

  20. RNA Extraction Without A Kit

    Type
    Blog Post
    ... and RNA is retained in the top, aqueous layer, while DNA and other proteins are retained in the interphase...
Showing: 221 - 240 of 552 results