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Showing: 1 - 20 of 87 results
  1. Rett Syndrome

    Type
    Collection
    ... Plasmid Collections Rett Syndrome Rett Syndrome Plasmid Collection...assemble a Rett Syndrome plasmid resource for the scientific research community. Background Rett Syndrome... mutations for Rett syndrome typically arise spontaneously, although in rare cases Rett syndrome can be...neurological defects of Rett syndrome. (Link opens in a new window) PMID: 29019980 Return to top Animal Models...Mutations PI Return to top External Resources Research Databases (Link opens in a new window) InterRett - international...genetic characteristics of Rett syndrome (Link opens in a new window) RettBase - MECP2 mutation database...database (Link opens in a new window) OMIM Rett Syndrome - Rett syndrome curated literature information (Link...
  2. Retrovirus Plasmids

    Type
    Collection
    ...discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses. γ-Retroviruses...FAQ) about retrovirus Popular γ-Retrovirus Envelope and Packaging Plasmids Common γ-retroviral envelope ...Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want a retroviral plasmid ...Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised of its genome and...mutagenesis). Read our γ-Retrovirus Guide for more information about: γ-Retroviral packaging systems Frequently...Retroviral packaging, envelope, and transfect plasmids Viral... Viral Plasmids γ-Retroviral Plasmids γ-Retroviral...
  3. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...suitable for FRET experiments to measure protein interactions or conformation changes, as well as FRET standards...Collections Fluorescent Proteins FRET Fluorescent Protein Guide: FRET More Fluorescent Protein Resources...using the cpFRET kit from the Pertz laboratory. Empty Vectors Encoding Fluorescent Proteins for FRET The following... Back to Top FRET Reference Standards The following plasmids have well-characterized FRET efficiency values... used as FRET standards to calibrate the numerous and disparate methods used to measure FRET. Plasmid ...Background Förster resonance energy transfer, or FRET , is a process by which energy is non-radiatively...developed for use as donor and acceptor pairs in FRET experiments. The efficiency of the energy transfer...
  4. Retrograde AAV viral preps

    Type
    Collection
    ...Ready-to-use retrograde AAV available from Addgene's viral service. Retrograde construct from Alla Karpova... Viral Service AAV Retrograde AAV Viral Service: Retrograde AAV As part of our Viral Service... the rAAV2-retro helper plasmid (Addgene #81070) . This serotype permits robust retrograde access and ...viral catalog items. Browse Available Retrograde AAV See our retrograde AAV based on the functional catagories...available plasmids. Browse all Retrograde AAV Depositor Comments The AAV retro helper plasmid was generated...producing ready-to-use viral vector preparations in a retrograde serotype from the Alla Karpova and David Schaffer...viruses would be helpful to the scientific community. Return to top...
  5. TALENs for Endogenous Human Genes

    Type
    Collection
    ...TGCGGGACGTGCGGGAGCggctgcaggcgtgggaGCGCGCGTTCCGACGGCA RET TAL2346 & TAL2347 TGAGTTCTGCCGGCCGCCggctcccgcaggggccaGGGCGAAGTTGGCGCCGA...
  6. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically...Campbell Zinc FRET-based zinc sensor for imaging in live cells (CALWY) Genetically encoded FRET sensors to...-2 Zn2+ FRET sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for...Cytosolic or Nuclear zinc-binding FRET biosensor (Zap) New alternately colored FRET sensors for simultaneous monitoring...Wolf Frommer ATP yAT1.03 FRET-based ATP sensor for yeast An Improved ATP FRET Sensor For Yeast Shows Heterogeneity...cyclic AMP) FRET sensors of cAMP levels for imaging in live cells Fourth-generation epac-based FRET sensors... for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion in Single beta Cells...
  7. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Collection . Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins...Endoplasmic Reticulum ER retention signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention...38770 pEF.myc.ER-E2-Crimson Endoplasmic Reticulum ER retention signal E2-Crimson Benjamin Glick 36204 ...36204 pmTurquoise2-ER Endoplasmic Reticulum ER retention signal mTurquoise2 Dorus Gadella 137804 ER-mNeonGreen...Endoplasmic Reticulum KDEL sequence mNeonGreen Dorus Gadella 137805 ER-mScarletI Endoplasmic Reticulum KDEL ...Endoplasmic Reticulum BiP ER signal sequence BFP Gia Voeltz 61807 Rtn4a-GFP Endoplasmic Reticulum Rtn4a AcGFP...Endoplasmic Reticulum Sec61 mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic Reticulum Sec61 BFP Gia...
  8. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PSPS1, PTP, REG RETN resistin ADSF, FIZZ3, MGC126603, MGC126609, RETN1, RSTN, XCP1 RETNLB resistin like ...ULBP4, bA350J20.7 RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L... 3 RAET1N Return to top BCR Signaling B cells mediate humoral immunity through the secretion of antibodies...chromogranin A (parathyroid secretory protein 1) CGA CHGB chromogranin B (secretogranin 1) SCG1 CKLF chemokine-like...FMRFAL NPPA natriuretic peptide precursor A ANF, ANP, ATFB6, CDD-ANF, PND NPPB natriuretic peptide precursor...precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor A/guanylate ...cyclase A (atrionatriuretic peptide receptor A) ANPRA, ANPa, GUC2A, GUCY2A, NPRA NPR3 natriuretic peptide...
  9. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...displays enhanced transduction of the retina following subretinal administration and enhanced transduction...transduction and lateral spread in the retina following subretinal injection. It was identified by John ...transduction and lateral spread in the retina following subretinal injection as compared to the parental...delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes were ...demonstrated to have enhanced transduction of the retina following intravitreal injection in mice and marmosets...trpYF) serotype shows enhanced transduction of the retina following intravitreal injection in mice and macaque...2009. High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther...
  10. Cre-lox system

    Type
    Collection
    ...Tamoxifen inducible Retroviral Jacks 24064 MSCV-Cre Cre and EGFP coexpression Retroviral Littman 24333 ins-cre_cryaa-Venus...inducible PGK Retroviral Lowe 33342 LGmCreER (non-self deleting) Cre-ERT2 PGK Retroviral Lowe 33344 pLEW100cre-EP1...pMSCVpuro-Cre Cre 5'LTR Retroviral Ge 34565 pMSCVhygro-Cre Cre and HygroR coexpression Retroviral Ge 34568 WZLneo-Cre...GFP coexpression CAG Retroviral Gage 49054 CAG-GFP/cre Cre-GFP fusion CAG Retroviral Gage 49056 AAV-GFP/...AAV Deisseroth 59701 pRetroQ-Cre-ERT2 Cre-ERT2 -Tamoxifen inducible cmvIE Retroviral Youle 59720 Nanog-CreER...6xHis T7 Bacterial Liu 63704 pRetroX GFP T2A Cre GFP and Cre tight TRE Retroviral Foijer 64769 pTW040 Cre ...neurons Retroviral Luikart 66693 pRubiG-T2A-cre GFP and Cre expression in newborn neurons Retroviral Luikart...
  11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Varies pBABE GFP - Retroviral gene expression pMKO.1 GFP - Retroviral shRNA... page . Retroviral Easy and safe to use pBabe plasmids - Mammalian retroviral gene ... retroviral shRNA expression MSCV-IRES-GFP - Mammalian retroviral gene expression...Transient expression pBABE-puro - Retroviral expression pWPXL - Lentiviral ...Backbones Neomycin (G418) Mammalian pBABE-neo - Retroviral gene expression Tet-pLKO-neo -... expression Puromycin Mammalian pBABE-puro - Retroviral gene expression pLKO.1 - TRC cloning...Tet-inducible lentiviral shRNA expression pMKO.1 puro - Retroviral shRNA expression pLenti CMV Puro...
  12. Bacterial Expression Systems

    Type
    Collection
    ...protein resources here . pCyPet-His 14030 FRET Patrick Daugherty FRET: Vector for arabinose inducible expression...found in pYPet-His . pYPet-His 14031 FRET Patrick Daugherty FRET: Vector for arabinose inducible expression... coli codon optimized FokI endonuclease domain. Return to Top Visualization and Tagging If you’d like ...fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein variant...fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein variant...fused to the MS2 binding sites within this plasmid. Return to Top Protein Purification Sometimes looking at...multiple constructs can be made and tested at once. Return to Top Controlled Expression It isn’t always beneficial...
  13. Luciferase Plasmids

    Type
    Collection
    ...transcriptional reporters. However, they are not secreted, so cells must be lysed or the substrate must ...to measure luminescence. Gaussia luciferase is secreted and more stable, making time course experiments...size. NanoLuc® Luciferase is small, bright, and a secreted version of the protein is available – these attributes...minescence‐based T wo‐ Hy brid) : Combines in vivo BRET with ex vivo luminescence-based co-immunoprecipitation...plants. Bioluminescence Resonance Energy Transfer (BRET) CalFlux VNT : An intracellular calcium sensor created...Antares and Enhanced Nano-lanterns) to allow for BRET-based in vivo imaging of multiple cellular processes...luciferase for ratiometric imaging David Nelson Return to Top Expression Constructs Luciferase driven ...
  14. Zhang Lab CRISPR Page

    Type
    Collection
    ...nickase) and single guide RNA 48137 : PX165; SpCas9 Return to top GeCKO Screening Library GeCKO are pooled...for more information. Full references are below. Return to top Cas9 SAM transcriptional activation plasmids...sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab...fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9 is a versatile...designed based on the target site sequence (20bp). Return to top References SpCas9 plasmids Multiplex Genome...CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini ...10.1038/nprot.2013.143. Epub 2013 Oct 24. PubMed . Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9...
  15. Plasmids for Stem Cell Research

    Type
    Collection
    ...MMLV-derived Retrovirus Human Yamanaka factors for generating human iPS cells; retroviral expression of...MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells; retroviral expression..., fully-differentiated adult somatic cells are returned to a pluripotent state using a cocktail of factors...11(3):373-86. Jaenisch Fibroblasts Hepatocytes Retroviral Mouse Direct conversion of mouse fibroblasts ...nature10323. Crabtree Fibroblasts Motor Neurons Retroviral Mouse/Human Conversion of mouse and human fibroblasts...2527-32. Wernig Fibroblasts Neural Stem Cells Retroviral Mouse Direct reprogramming of fibroblasts into...Cell Stem Cell. 2019 Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron ...
  16. Viral Vectors

    Type
    Collection
    ...expression. γ-Retrovirus Plasmids | Guide γ-retroviruses, commonly referred to as retroviruses, are the most...this infectivity is limited to dividing cells. Retroviruses integrate permanently into the host genome, ... be produced. Each of these plasmids, however, retains the properties of any typical plasmid and thus ...DNA >8 Kb 105 Dividing/Non-dividing No High γ-Retrovirus Stable RNA <8 Kb 80-130 Dividing Yes Moderate...genome integration. Community Resources Nolan Lab Retroviral Systems Trono Lab Lentivector Toolbox Guide to...
  17. Open Enzyme Collection

    Type
    Collection
    ...Tth DNA Ligase 165574 pOpen-HSlig3 DNA Ligase III Return to top DNA Polymerases Addgene ID Plasmid Gene/...Polymerase 165573 pOpen-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid...165567 pOpen-XbaIR XbaI 165568 pOpen-NcoIR NcoI Return to top Reverse Transcriptases Addgene ID Plasmid...Thermus thermophilus (Tth) Reverse Transcriptase Return to top RNA Polymerases Addgene ID Plasmid Gene/...Polymerase 165576 pOpen-T3gene1 T3 RNA Polymerase Return to top Other Enzymes Addgene ID Plasmid Gene/insert...165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources Bacterial Protein Expression...
  18. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ... enable us to purify specific genomic regions retaining molecular interactions and identify molecules ... TAL protein recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance... TAL protein recognizing a telomere repeat. A retroviral expression vector with the neomycin resistance... Fujita T, Persson CU, Eriksson P, Veerla S, De Preter K, Speleman F, Fujii H, Påhlman S, Mohlin S. Biochem...Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using...associated with an IFNgamma-responsive promoter by a retroviral expression system for enChIP using CRISPR. Fujita...
  19. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-Calretinin [L122/68R] Calretinin Human Mouse IgG2a 177469 Anti-Calretinin [L122/6R] Calretinin Human...-CALB2/calretinin [L122/68R-2b] CALB2/calretinin Human Mouse IgG2b 206642 Anti-CALB2/calretinin [L122/...Theta Human Mouse IgG2a 188159 Anti-Calretinin [L122/144R] Calretinin Human Mouse IgG2a 188160 Anti-TASK1... NSD1 Human Mouse 206780 Calretinin scFv [L122/144] L122/144 scFv Calretinin Human Mouse 206781 GABA(A..., HEK293, COS, CHO), from which the R-mAbs are secreted into the culture media. For high level expression...L122/6R-2b] CALB2/calretinin Human Mouse IgG2b 206643 Anti-GAD65/67 [L127/12R-2b] GAD65/67 Human Mouse IgG2b...
  20. Recombinases AAV Preps

    Type
    Collection
    ...none 1, PHPeB Zeng *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...1, 8, 9, rg* Engel , Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none 1 Cepko 69571 pAAV-EF1a-C-CreintG EF1a...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...
Showing: 1 - 20 of 87 results