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We narrowed to 609 results for: GAN

Showing: 241 - 260 of 609 results

  2. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Chromatography columns containing appropriate immobilized ligands are incubated with cell culture supernatant containing...antibodies. The antibodies bind the immobilized ligands and can be eluted once the other materials have...incubated with the cell suspension. Antibodies bind ligands on the beads and a magnet is used to separate the...

  3. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...slices, which are easier to expand than, say, a C. elegans (Damstra, et al. 2022; Yu, et al. 2020). With any...//doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging of Chemical...Wassie AT, et al (2020) Expansion microscopy of C. elegans. Elife 9:e46249. https://doi.org/10.7554/elife.46249...

  4. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans...Enzymol. 1983;100:293-308. PubMed PMID: 6312261. 2. Keegan, L., Gill, G., and Ptashne, M. Separation of DNA...

  5. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...

  6. Lab to Office Culture Shock

    Type
    Blog Post
    ... number of career paths--industry, nonprofit organization, law office, journal editor, etc.--so everyone...around the world, such as BosLab here in Boston. Organizations like this one are always looking for scientists... own independent research!). There are also organizations like Science from Scientists, which try to bring...

  7. How to Write a Scientific Review Article

    Type
    Blog Post
    ...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your Next Manuscript 6 Tips...

  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... that the drug prevents the growth of a living organism. As for multi-drug resistance: definitions vary...vary in the literature, but generally organisms with multi-drug resistance are resistant to three or more...is an intense amount of competition between microorganisms for available resources. This competition drives...

  9. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...over-express coding sequences from a given cell type or organism, have been used in a similar manner to Cas9 activators...lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the...Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden...

  10. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...distinct subcellular environments, including the organelles that compose the secretory pathway, endocytic...formation, and proteolytic cleavage. FPs localized to organelles are equally susceptible to these non-native modifications... Lee Snapp. "Fluorescent proteins in cellular organelles: serious pitfalls and some solutions." DNA and...

  11. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...non-human primates [7, 8], zebrafish [9], and c. elegans [10]. If used properly, optogenetics is sure to...Cellular and Molecular Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the...neural activity in freely moving Caenorhabditis elegans." Nature methods 8.2 (2011): 147-152. PubMed PMID...

  14. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...the observation of proteins in living cells and organisms. Both components of the chimera are encoded by...). However, these assays are qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini...hindrance by the fluorescent protein. Thus, scientist began using relatively long linkers between the protein...

  15. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... were identified using systematic evolution of ligands by exponential enrichment (SELEX). After 4-6 rounds...single molecules and for live-cell imaging in C. elegans. Other groups have used Mango to study RNA-protein...PubMed Central PMCID: PMC3852148. Warner, Katherine Deigan, et al. "Structural basis for activity of highly...

  16. Luciferase Plasmid Collection

    Type
    Collection
    ...responsible for bioluminescence found in a variety of organisms, ranging from bacteria to aquatic animals to insects...

  17. Caltech Systemic Capsids

    Type
    Collection
    ...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...

  18. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...has also been implemented in a variety of other organisms, such as sphingomonads and bacillus.  ADH1 The...shown that the Q system is also functional in C. elegans. Subsequent work by the Luo and Potter labs has...repressible binary expression system in Caenorhabditis elegans.” Nat Methods 9(4) (2012): 391-5. PubMed PMID: ...

  19. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...signaling molecules, transcription factors, and ligands. The presenter then showed which genes they had...that were essential for the activity of different ligands. Such knowledge could be very useful for drug discovery... to block the cellular response to a particular ligand (such as when a component of a microbe or a cytokine...

  20. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... blood stream to commute to and invade multiple organs, and most importantly escape the immune mechanisms...Cancer cells also produce programmed cell death ligand (PD-L1) that inhibits the cytotoxic activity of...environments for tumor cells spreading to other organs during metastasis. Evading Recognition Tumor cells...
Showing: 241 - 260 of 609 results