Viral Vectors 101: Biosensors
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Blog Post
...and are ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics...
We narrowed to 402 results for: Mut
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GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully... -
Viral Vectors 101: Viral Applications
TypeBlog Post... have been engineered to introduce frameshift mutations in genes of interest, turning expression off permanently... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post... contain the expected features and are free of mutation. Once the samples arrive, each group of plates... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...essentially any gene,” she says. The technical leap from mutating and deactivating a gene or genes to selectively... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...light-sensitive protein QuasAr2 and systematically mutated it. Using a robotic picking approach to select ... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post... the protein. To monitor autophagy, the Pho8Δ60 mutant protein is expressed. Pho8Δ60 localizes to the ... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...early-onset cataracts, is characterized by point mutations in the Iron Response Element (IRE) RNA motif of... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...domain of a Ciona intestinalis voltage sensor and a mutated version of the pHLuorin fluorescent protein. This... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth... -
Plasmids 101: The protein expression toolbox
TypeBlog Post... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression... -
All in a Twist: dsRNA
TypeBlog Post...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation... -
Antibodies 101: Affinity Reagents
TypeBlog Post...fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used for assembling ... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating... -
Plasmids 101: Cre-lox
TypeBlog Post...potentially recombine. To account for this, alternate mutant versions of the loxP site have been created, which... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post... assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination ... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...promoter of the 5’ long terminal repeat (LTR) is mutated in third generation systems such that it no longer... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together...