We narrowed to 363 results for: gne

1. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   Blog Post

   ...ion channels. Similarly, chemogenetic tools are designed channels that are chemically activated. Both are...

2. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   Blog Post

   ...heterodimeric fusion of wild-type TadA and TadA7.10, they designed one set of ABE8’s similarly (ABE8.x-d). In order...

3. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   Blog Post

   ...doi.org/10.1038/s41467-021-25991-9. Spruijt CG, Gnerlich F, Smits AH et al. (2013) Dynamic readers for ...

4. Plasmids 101: Modular Cloning

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   ...cloning, or MoClo, is a systematic cloning strategy designed by synthetic biologists to perform large scale...

5. Viral Vectors 101: Calcium Sensors

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   Blog Post

   ... There are several calcium reporters specially designed for the aims and requirements of in vivo studies...

6. High Complexity Golden Gate Assembly with NEB’s DAD

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   Blog Post

   ... a stochastic search algorithm rather than pre-designed sets, so identical searches can return multiple...

7. Viral Vectors 101: Virus Safety

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   Blog Post

   ...that are built into how something functions, were designed for this purpose. One such control is dividing...

8. Antibodies 101: Choosing the Right Isotype

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   Blog Post

   ...your application, of course!) When the stars are aligned and the lab gods are smiling down at you, you may...

9. Hot Plasmids and Viral Preps - March 2021

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   Blog Post

   ...for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab produce different levels of dCas9 expression...

10. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post

    ...promoters with the help of epigenetic analysis and designed using stretches of DNA that were transcriptionally...

11. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post

    ...Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014...

12. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post

    ... try amplifying the viral vector with bacteria designed to minimize rearrangements such as NEB Stable ...

13. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post

    ...to examine Cpf1 off-target editing. First, they designed gRNAs with single and double mismatches throughout...

14. Plasmids 101: FLEx Vectors

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    Blog Post

    ...switches? FLEx (or “flip-excision”) switches were designed as a genetic tool for researchers to conditionally...

15. Plasmids 101: Terminators and PolyA signals

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    Blog Post

    ...Plasmids designed to express genes in a given host cell type are generally broken down into two broad...

16. Tips for Titering Your Lentiviral Preps

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    Blog Post

    ...virus will not only ensure that your infection is designed in the best possible way, but it may also save...

17. Gaining Leadership Skills Volunteering at a Professional Organization

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    Blog Post

    ... the overall voice may not always be perfectly aligned with my personal viewpoint but, as a leader in ...

18. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    Blog Post

    ...resolved. If there are any forms that need to be signed, or manuscripts that need to be reviewed, bring...

19. Protocol - Bacterial Transformation

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    Protocol

    ...Because of this, nearly all plasmids (even those designed for mammalian cell expression) carry both a bacterial...cause the cells to take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture to induce...

20. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ...amazing project. You’ve written your protocols, designed your experiments, and prepared your reagents. ...