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We narrowed to 276 results for: guide rna expression plasmid

Showing: 241 - 260 of 276 results

  1. Tips for arabidopsis transformation

    Type
    Blog Post
    ...plant plasmid resources at Addgene  Choosing an expression system Choosing an appropriate expression vector....org Find plant plasmids Visit our plant resource page Deposit arabidopsis plasmids with Addgene ...., 2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the Dinneny...this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium tumeficans - a...options, but here are a couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway...for visual selection of transformants based on expression of RFP in the seed (Emami, Yee and Dinneny, 2013...culture you are growing definitely contains your plasmid. Grow lots of Agrobacterium: The more agrobacterium...

  2. Kazuhiro Oka Lentiviral Vectors

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    Collection
    ... may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and Resources Addgene Blog: ...: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors...reporter plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing...-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1...pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre under ...pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1-Fon-copGFP 72260 Expresses copGFP from...

  3. CRISPR References and Information

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    Collection
    ...Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR... CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression: pAC2 , ...129 KB Sontheimer gRNA design and cloning All-in-one plasmid containing expression cassette for NmCas9 and...597 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330)... for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription...endogenous proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available for CREB1, ATF1, GABPA, and RAD21... generation; gRNA design; HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180...

  4. Worm Expression Resources

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    Collection
    ... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...'s worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous...backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described below...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the...

  5. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    Blog Post
    ...their seminal work on micro RNAs in C. elegans. The C. elegans work on small RNAs informed and drove work ...modular toolkits build single plasmid targeting vectors that encode the gRNA transcript and the repair template...step as simple as heat-shocking to induce the expression of a Cre recombinase contained within the knock-in... C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives It is interesting to...using modified Cas9 proteins to regulate gene expression (CRISPRi/CRISPRa), visualize specific genomic...Addgene's CRISPR Resources Learn about gRNA design and gRNA optimization for your CRISPR experiment ...depending on perspective – to work with inefficient sgRNAs in my initial direct screening efforts, which were...

  6. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post
    ...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged... whatever molecule you choose (Figure 1). Just express the fusion protein and add a specific ligand! A...better detect dim structures or low levels of expression with the same imaging workflows you already use...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...Dyeing Through Chemistry Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands GCE4All...10.1038/s41467-023-39655-3 Wilhelm, J., Kühn, S., Tarnawski, M., Gotthard, G., Tünnermann, J., Tänzer, T.,...

  7. Lentivirus Plasmids

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    Collection
    ...lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti...

  8. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...Designing the donor plasmid Figure 2: Donor plasmid design strategy. A donor plasmid contains 1 kb homology...mutations in crRNA target sites found within the homology areas of the plasmid to prevent the plasmid from being... the tag. Created with BioRender.com. Plasmids, plasmids everywhere To find out more about gene edited...CRISPR Tagging Plasmids Browse the Fluorescent Protein Collection Check out the CRISPR Guide Pages .../Cas9 (Cas9 protein and the crRNA-tracrRNA complex) and the donor plasmid into the cells we use electroporation...Further, the Yamamoto PITCh system plasmids provides an alternative to HDR as the double-strand break ...expensive for many labs. We’ve created and tested plasmids that use CRISPR/Cas9 to endogenously tag a wide...

  9. TALEN Plasmids and Kits

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    Collection
    ...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built... For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting...Maria-Elena Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors...pTAL4. The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including...

  10. Antibodies 101: Reading a Flow Plot

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    Blog Post
    ...population. It can be used to measure protein expression, identify rare cells, or even sort out single...using the location of the cell populations as a guide  (Figure 3.) You can see that in our dot plot, we... Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to Antibodies...PLOS ONE, 12, e0180664. https://doi.org/10.1371/journal.pone.0180664 Boix, F., Llorente, S., Eguía, J., Gonzalez-Martinez...infection in liver and kidney recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500...PloS One, 10, e0137550. https://doi.org/10.1371/journal.pone.0137550 More resources on the Addgene blog Conventional...

  11. Plasmid Collections

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    Collection
    ...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 161,255 plasmids . We've...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Mammalian RNAi Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...Curated Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion... Expression Kits Kits are groups of plasmids that have been packaged together because they are intended...

  12. Viral Vectors 101: Virus Safety

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    Blog Post
    ...transfer plasmid), the envelope (Env) gene on a separate plasmid (envelope plasmid), and the polymerase ...different plasmids. For a retroviral system this looks like the genetic cargo in one plasmid (the transfer...Gag structural protein on yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection...Addgene viral services Addgene Biosafety Guide Addgene Viral Plasmids Which Type of Virus Should I Use? ... transfection of all three plasmids into a producer cell line, such as HEK-293T, production of viral particles... and Pol genes are not encoded in the transfer plasmid, the component that physically integrates into ... retroviral packaging components onto distinct plasmids for safety.    Viral cargo-specific safety...

  13. Rinehart Lab Phosphoprotein Reagents

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    Collection
    ...SepOTSγ Plasmid 68285 SepOTSδ Plasmid 68286 SepOTSϵ Plasmid 68287 SepOTSζ Plasmid 68288 SepOTSη Plasmid...SepOTSθ Plasmid 68290 SepOTSι Plasmid 68291 SepOTSκ Plasmid 52054 SepOTSµ ( aka. B40 OTS) Plasmid 68294 ...68294 SepOTSν Plasmid 68295 GFP E17TAG Plasmid 68296 GFP S2TAG Plasmid 68297 GFP Q157TAG Plasmid 68298 GFP...Amp) Plasmid 68300 MBP-MEK1 Plasmid 68301 MBP-MEK1 S218TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68305...pKD-SepRS-EFSep) Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 68283 SepOTSβ Plasmid 68284 SepOTSγ... 68306 C321.ΔA.Δserb.Amp S Plasmid 68292 SepOTSλ Plasmid 68307 SupD Plasmid 34623 SepOTSα SepRS/EF-Sep...( 188537 ) is a ROP minus plasmid and is not compatible with other plasmids containing high copy ColE1...

  14. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...advantage of DNA sequencing to turn mAb clones into expression constructs to be conveniently modified and engineered...atlas will enable scientists to have an intuitive guide for checking their staining and neuroanatomy without...Biol 13(6): e1002165. https://doi.org/10.1371/journal.pbio.1002165 More resources on the Addgene blog Antibodies...Antibodies 101: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ...

  15. Deep Dive: qPCR

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    Blog Post
    ...quantification of mRNA, which can be used as a proxy measure for differences in protein expression. (If you're...you can assume that qPCR can accurately detect expression differences of twofold or greater with good statistical...shown to have as much as sixfold variation in expression levels reported (Biassoni, 2014), so you’ll want...Housekeeping Genes as Reference for qPCR Gene Expression Analysis During iPS Reprogramming Process. Sci...; Higgins PJ; Crawford DR. Control selection for RNA quantitation. Biotechniques. 2000 Aug;29(2):332-7...Germain H. Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable...blood glucose-regulated and can up-regulate the expression of GAPDH. Biochem Biophys Res Commun. 2001 Mar...

  16. Antibody Plasmid Collection

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    Collection
    ...our Antibody Guide ! Cloning and Expressing Antibodies The following table highlights plasmid collections... Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies, nanobodies...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant...partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made...created by cloning antibody components into plasmids and expressing these in bacteria, mammalian cells, yeast...collections that can be used to create and express plasmid-based antibodies. Description Article PI One-step assembly... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...

  17. Antibody Production

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    Collection
    ...mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding... low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...chains. Antibodies are expressed for 7 days and then purified from the supernatant using Protein A or Protein...tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue Atlas (Link opens...retained. If the target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody), the target...target antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments were...species-specific HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the...

  18. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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    Blog Post
    ...depositing plasmids pre-publication Find molecular biology protocols Find eBooks on plasmids, CRISPR, viral...changes in root-to-shoot ratio in Arabidopsis, (2) expression patterns in plants with enhanced sodium accumulation...scientific journal. The peer-review is a service that we, as scientists, provide for journal editors to...been peer-reviewing journal submissions since 2015 for a wide variety of journals. Since then, I developed...the submitted paper in addition to the journal-specific guidelines.  Peer-review step 1: The first read ...whether work is suitable for publication in their journal. The early peer-review attempts took place at the...submission, then for the submission to the subsequent journal.  As a postdoctoral researcher studying plant development...

  19. Which Fluorescence Microscopy Technique is Best for Me?

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    Blog Post
    ...Resources Online Microscopy Primer on the Molecular Expressions Website Other major microscopy manufacturers ...PubMed PMID: 12624904. 5. Power RM, Huisken J. A guide to light-sheet fluorescence microscopy for multiscale... Find Empty Fluorescent Protein Backbones Find Plasmids for Optogenetics ...photon microscopes. Total Internal Reflection Microscopy (TIRF) Total Internal Reflection Microscopy (TIRF... are the main focus of the experiment, a Total Internal Reflection Fluorescence (TIRF) microscope can ...2015). PubMed PMID: 25998828. 4. Axelrod D. Total internal reflection fluorescence microscopy in cell biology...

  20. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...PMCID: PMC3810607. 2. Bajar, Bryce T., et al. "A Guide to Fluorescent Protein FRET Pairs." Sensors 16.9... create fusions with a gene of interest. These plasmids will be most useful for constructing intermolecular...including fluorescent proteins, has led to the alternative acronym Fluorescence Resonance Energy Transfer... endogenous state that you want to measure (overexpressing a gene or protein after transfection). Future...stress sensor for specific proteins in situ." FEBS journal 275.12 (2008): 3072-3087. PubMed PMID: 18479457...Biosensors and Intravital FRET Microscopy." Biophysical Journal 111.6 (2016): 1103-1111. PubMed PMID: 27475975....
Showing: 241 - 260 of 276 results