We narrowed to 266 results for: ogn

...work, but part of good experimental design is recognizing that we’re biased and taking steps to prevent...

...wash, add dye-conjugated secondary antibody that recognizes the primary, wash again, then image. But here...

...had used plasmids from this company before and recognised the logo, but didn’t know much else. During the...

...also honored IMP Mentors & Mentees with awards recognizing mentee Lucía Ramos (University of Valencia, Spain...

...Charpentier for their pioneering CRISPR/Cas9 work (now recognized by the 2020 Nobel Prize in Chemistry), and Victor...

..., Orchidée, et al. "A dynamic map of antigen recognition by CD4 T cells at the site of Leishmania major...

...the target marker. Looking at Table 1, we can recognize a couple of fluorophores from Figure 1. For example...

...epitopes such that immune complexes will no longer recognize them. Sometimes, users will combine methods and...

...analyzing the neural circuits of behavior. Trends in cognitive sciences. 2011;15(12):592-600. doi: 10.1016/j....

...by creating BE variants with other Cas9s that recognize different PAMs. Their computational tool for predicting...

...resolution? The prime editing heteroduplex is likely recognized as an MMR intermediate, and the MutSα–MutLα complex...

...requirements: (1) that you have international recognition for your outstanding achievements in your specific...

...terminal repeat sequences, which the PB transposase recognizes. Following reprogramming, transgenes can be removed...

...This paCas9-1 light-inducible system was able to recognize the same PAM and had similar targeting specificity...

... the repair template, and the locus cannot be recognized by the spacer-derived crRNA. However, if recombineering...

...cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase. Nature Biotechnology. ...

...capable of recognizing and binding to epitopes on antigens, they are also capable of recognizing and binding...source secondary recognizes], e.g. , “goat anti-rabbit.” Choose a secondary that recognizes your primary ...proteins, naturally produced by the immune system to recognize and bind foreign bodies. Antibodies can be used...proteins of interest. The protein that an antibody recognizes is called an antigen, while the specific binding... probability that an antibody will be able to recognize and bind to any given antigen. A mature B cell...specificity in antibody-based methods. Antibodies can recognize and bind other antibodies using epitopes present...important to choose a monoclonal antibody that will recognize an available epitope on your protein of interest...

...regions, as it recognizes TTTV (V = A, C, or G) PAM sites. Engineered versions of Cas12a recognize different... mutations in multiple domains to expand PAM recognition, increase specificity, and decrease off-target...Faecalibaculum rodentium ) has increased fidelity, and recognizes NNTA, a PAM sequence often found in plants, expanding...mechanisms, including blocking crRNA loading or PAM recognition, inhibiting Cas9’s endonuclease domain, or inducing...EnCas12a, an engineered version of AsCas12a that recognizes TYCV and TATV PAM sites. The DETECTR platform...using Cas13 Cas13 is a type VI CRISPR enzyme that recognizes ssRNA. Type VI enzymes, which include Cas13a ...crRNA precursors to mature crRNAs. Upon ssRNA recognition by the crRNA, the target RNA is degraded. In ...

...requires an associated sigma factor for promoter recognition and binding. On the other hand, the process in...binding to the promoter region. Each sigma factor recognizes different core promoter sequences. Figure 2. ...eukaryotes. Operons consist of a promoter (which is recognized by the RNA polymerase), an operator (a segment...

...distance away from the recognition sequence. Cutting distal to their recognition site allows for the creation...the insert and plasmid in such a way that all recognition sequences are removed from the final product,...