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...drum. They have a wide variety of possible genome types that can be either double stranded (ds) or single...them far safer to work with than virions. The four types of viral vectors (which many researchers will informally...

...antibodies within certain classification groups called isotypes.  Why does isotype matter? Isotype matters when...secondary. If you were to change one of the antibody’s isotypes, however, you could then use 2 different secondaries...

...expression in target cells. While the various AAV serotypes can be used to achieve different patterns of expression... to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV inventory for the...

... et al. tested iTOP in a series of primary cell types using Cre and various loxP reporters. In both mouse...primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal and gut...

...comparing the transduction patterns of different AAV serotypes. To help make this easier for scientists, we now...testing. These samples are available in various serotypes of two popular control AAV encoding GFP. Check...

...improved transduction profiles in different AAV serotypes both in vitro and in vivo when compared to the...blood-brain-barrier. In summary, exo-AAVs seem to target cell types with a similar specifically as conventionally produced...

...Postdoctoral Research Fellow with Pamela Silver.  Most types of biological motion (whether endocytosis, vesicle...explanation of R-bodies, check out this video. Most types of biological motion (whether endocytosis, vesicle...

...sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so off-target...systems ensures that they can be used with many types of proteins, including Cas9 and dCas9-transcription...

...Connie Cepko to learn about her management style. Recipes for delegation success Remember that there are ...authority, responsibility and accountability? What types of issues do you want to know about right away? ...

...spanning 20 years of research. DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.... 2015 Jan 15;29(2):197-211. DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes...

...and gamma (Gγ). G proteins are divided into four subtypes, based on their Gα subunit: Gαs, Gαi/o, Gαq/11...GPCRs show a propensity to associate with certain subtypes, although most are able to signal through more...

...expressing plasmid. Envelope proteins determine which types of cells a virus can infect. The VSV-G envelope ...enables this virus to infect many different cell types so it is useful in a wide range of applications....

...completely ineffective or results in low penetrant phenotypes that are hard to study. To overcome this, most...RNAi vector that leads to significantly stronger phenotypes than standard RNAi protocols without the need...

... creating and sharing knowledge, related to the types of materials we help distribute and the research...Then we format, do a final review to catch those pesky typos, and hit publish. Making content is an iterative...

...experiences, but the increased binding can mask the epitopes antibodies recognize. It is therefore best to ...for one that has been validated in multiple assay types. Remember that ChIP uses native proteins (so antibodies...

... packaging, and transfer) are supplied by three types of plasmids, which are cotransfected into the 293T...flexibility to pseudotype γ-retrovirus using different envelopes to modify tropism. Briefly, different envelope ...

...relatively low retrograde transport efficiency in most serotypes. Some viruses (e.g., rabies) naturally exhibit... be transduced equally well by AAV-retrograde serotypes (Skorput, 2022, Albaugh, 2020, Tervo, 2016).  ...

...Tools blog can help you learn about the different types of viruses and pick one for your experiment. Research... of transduction in difficult-to-transfect cell types ranging from primary to embryonic stem cells. Viral...

... with all the new biotechnology.” These are the types of inquiries that routinely hit the inbox at the...ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant housing...

...member. But, at Addgene, we use seven different types of “plates” (Fig. 1). When you have to tell an engineer... barcoded (this is pretty Addgene specific) The types or purposes of each given container, when applicable...