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  1. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...antibodies within certain classification groups called isotypes.  Why does isotype matter? Isotype matters when...secondary. If you were to change one of the antibody’s isotypes, however, you could then use 2 different secondaries...

  3. New and Upcoming Viral Vectors - December 2019

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    Blog Post
    ...expression in target cells. While the various AAV serotypes can be used to achieve different patterns of expression... to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV inventory for the...

  4. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...deliver a genes of interest to a variety of cell types in-vivo. These are widely used by the optogenetics...revolutionized how researchers make knockouts and other types of genome edits, but, even with CRISPR, it isn’t...

  5. Using Addgene's New Viral Service for Your Research

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    Blog Post
    ...re also distributing AAV preps with particular serotypes that can be used to deliver fluorescent proteins...future users. We’re excited to be distributing new types of materials. At the time of publication we’ve already...

  6. Another Pathway into Cells: iTOP

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    Blog Post
    ... et al. tested iTOP in a series of primary cell types using Cre and various loxP reporters. In both mouse...primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal and gut...

  7. New and Upcoming Viral Vectors - June 2019

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    Blog Post
    ...comparing the transduction patterns of different AAV serotypes. To help make this easier for scientists, we now...testing. These samples are available in various serotypes of two popular control AAV encoding GFP. Check...

  8. Improving Transduction Efficiency with Exosome AAVs

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    Blog Post
    ...improved transduction profiles in different AAV serotypes both in vitro and in vivo when compared to the...blood-brain-barrier. In summary, exo-AAVs seem to target cell types with a similar specifically as conventionally produced...

  9. R Bodies: Membrane-Rupturing Microscopic Tools

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    Blog Post
    ...Postdoctoral Research Fellow with Pamela Silver.  Most types of biological motion (whether endocytosis, vesicle...explanation of R-bodies, check out this video. Most types of biological motion (whether endocytosis, vesicle...

  10. Synthetic Photobiology: Optogenetics for E. coli

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    Blog Post
    ...sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so off-target...systems ensures that they can be used with many types of proteins, including Cas9 and dCas9-transcription...

  11. Management for Scientists: Delegating is Key

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    Blog Post
    ...Connie Cepko to learn about her management style. Recipes for delegation success Remember that there are ...authority, responsibility and accountability? What types of issues do you want to know about right away? ...

  12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post
    ... packaging, and transfer) are supplied by three types of plasmids, which are cotransfected into the 293T...flexibility to pseudotype γ-retrovirus using different envelopes to modify tropism. Briefly, different envelope ...

  13. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...relatively low retrograde transport efficiency in most serotypes. Some viruses (e.g., rabies) naturally exhibit... be transduced equally well by AAV-retrograde serotypes (Skorput, 2022, Albaugh, 2020, Tervo, 2016).  ...

  14. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...Tools blog can help you learn about the different types of viruses and pick one for your experiment. Research... of transduction in difficult-to-transfect cell types ranging from primary to embryonic stem cells. Viral...

  15. How Addgene Creates Content

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    Blog Post
    ... creating and sharing knowledge, related to the types of materials we help distribute and the research...Then we format, do a final review to catch those pesky typos, and hit publish. Making content is an iterative...

  17. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ...and gamma (Gγ). G proteins are divided into four subtypes, based on their Gα subunit: Gαs, Gαi/o, Gαq/11...GPCRs show a propensity to associate with certain subtypes, although most are able to signal through more...

  18. A Deep Dive into BioBuilder

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    Blog Post
    ... with all the new biotechnology.” These are the types of inquiries that routinely hit the inbox at the...ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant housing...

  19. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post
    ...expressing plasmid. Envelope proteins determine which types of cells a virus can infect. The VSV-G envelope ...enables this virus to infect many different cell types so it is useful in a wide range of applications....
Showing: 241 - 260 of 513 results