We narrowed to 322 results for: phy

1. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

   Type

   Blog Post

   ...special category, as has been the ‘labor-of-love’ philosophy of academic science to date. Advocacy opportunities...

2. Plasmids 101: FLEx Vectors

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   Blog Post

   ...Double-floxed Orientation) and could be used to study the physiology or behavior of certain cell populations.  Acknowledgement...

3. Illuminating Epigenetics with A FRET Based Biosensor

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   Blog Post

   ...becomes. This prevents the cellular machinery from physically interacting with the genes in the compacted region...

4. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   Blog Post

   ...B-thalassemia, Sickle Cells Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used...

5. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

   Type

   Blog Post

   ...but close together in terms of where they are physically located. DNA is then sheared into pieces small...

6. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

   Type

   Blog Post

   ... being a promising approach for treatment or prophylaxis of COVID-19. Researchers identified nanobodies...

7. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   Blog Post

   ... from fluorescent jellyfish, bioluminescent phytoplankton, and colorful corals, to name a few. What’s ...

8. 10 Basic tips for mammalian cell culture

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   Blog Post

   ...disregulation on HeLa cells." Journal of applied phycology27.6 (2015): 2321-2332. 3. Contamination, Ileana ...

9. Transferable Skills: Negotiation

   Type

   Blog Post

   ...purchases do we prioritize in a lab with both electrophysiologists and molecular biologists? What is their...

10. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    ... ddPCR- and qPCR-based titering assays measure physical titers, meaning the concentration of viral particles...

11. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post

    ...cells participating in development or other physiopathological events to which the chosen promoter responds...

12. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post

    ...control (NTC) should always be included. While the physical titer generated by qPCR is useful, I test a few...

13. Experimenting with New Careers while in Grad School

    Type

    Blog Post

    ...erlenmeyer flask with gold glitter spray paint to make a trophy for the winner. We promoted the contest for about...

14. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post

    ...Medium Medium Medium Difficulty Easy Easy Easy Physical transfection   Electroporation / Nucleofection...

15. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    ...in plants because of the auto-fluorescence of chlorophyll, so we looked at newer fluorescent proteins that...

16. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...applications in imaging living cells and tissues”  Physiol. Rev. 90:1103. Wu et. al. (2011) “Modern fluorescent...

17. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    ...with the BioBricks Foundation to distribute the physical DNA samples, for when that's the preferred mode...

18. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post

    ...Provost’s course recently used a combination of phylogenetic analysis, structural prediction, and bench work...

19. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    ... we have used quantitative PCR to evaluate the physical titer of our preps. We often repeat this assay...

20. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular Biology....