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TypeBlog Post...plot. In this post, I’ll walk you through how to interpret the antibody readout on a flow plot. Note that...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...
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Improved Plasmid Maps Powered by SnapGene
TypeBlog Post... and sequence displays are now much easier to interpret and analyze at a glance. For a quick look at just...“Analyze Sequence” button. The maps hosted here retain all the features shown in the static maps discussed... -
How Addgene Creates Content
TypeBlog Post...quickly." Final design (right). Immediate and immense regret of the graphic designer not shown. However... and knowledge changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog Post...website resources that best match a user’s query, and return the matching resources in an order that makes sense...expose as filters and designed the search engine to return only the filters that are relevant to a specific... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...cellular transduction events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors to... -
RNA Extraction Without A Kit
TypeBlog Post... and RNA is retained in the top, aqueous layer, while DNA and other proteins are retained in the interphase... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...fiction story that doesn’t require any detailed retention of information or formulation of new knowledge...mental bandwidth. Jargon Learning, using, and retaining new vocabulary takes time and effort (otherwise... -
What's New in CRISPR - November 2021
TypeBlog Post...for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...demonstrate SEND as a modular system for packaging, secretion, and delivery of specific RNAs, such as Cre mRNA... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog Post...plan for a career outside academia (that would be pretty much all scientists), a LinkedIn profile is absolutely...rules of good resume writing. Include examples of concrete accomplishments in both the technical and “plus... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post...growth factor-β signaling, possibly inducing the secretion of growth promoting proteins, such as human growth...microenvironments! Take myoepithelial cells for instance. They retard tumor formation by inhibiting cell growth, invasion... -
Plasmids 101: The protein expression toolbox
TypeBlog Post...integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and have...to permanently induce expression? Check out our Retro expression vector and Lenti expression vector. ... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog Post...variable gene expression can make data hard to interpret or can interfere with the precision needed in ...only two fold. Moreover, gene expression levels returned to the baseline after 3 hours with the stabilized... -
Antibodies 101: Fab Fragments
TypeBlog Post...few commonly available different fragments that retain antigen binding (Figure 2): F(ab')2 - These fragments...fragments are produced by proteolytic digestion that retains an antibody’s disulfide bonds within the hinge ... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...gender analysis and 2) provides case studies with concrete examples of how gender analysis leads to discovery...design. But eyes have been opened—and we cannot return to a world that ignores gender. Many thanks to... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...Technology has been “working on new experimental and theoretical methods to push the scale of genetic engineering...paper, there actually were not many hurdles. It was pretty straightforward surprisingly. So I think a nice... -
Fluorescent Protein Guide: Activity Regulation
TypeCollection...More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics... -
Michael Davidson Fluorescent Protein Collection
TypeCollection...More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The lab of... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post... domain can edit the short single-stranded DNA stretch that is accessible. Image from Gaudelli et al.,... editors to correct mutations in stem cells and return the corrected cells back to the patients. For example... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...quantification of various intermediate steps, a sizing electrophoretic gel, and qPCR quantification of the final libraries...assembly. Most of the time, these plasmids are returned as one or two partial assemblies. If we obtain... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog Post...than pre-designed sets, so identical searches can return multiple answers. You might want to take advantage...fragments, or defined split regions. SplitSet will then return the highest-fidelity overhang set it can find for...