We narrowed to 531 results for: sequence

...transcriptional activator that binds to UAS enhancer sequences found in DNA. It then recruits transcription machinery... genes and siRNA encoded downstream of the UAS sequence are only expressed when Gal4 is expressed. Gal4...multiple genes under the control of UAS enhancer sequences. For example, if you discovered five new genes...

.... Piecing together multiple PCR products using sequence homology is also common. Overlap extension PCR... to splice together two PCR amplicons based on sequence homology, and the subsequent product is then digested...fragment ends have secondary structure or too much sequence similarity. REPLACR-mutagenesis: Letting bacteria...

...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...

...transformation, including regulatory elements, coding sequences, basic CRISPR parts, and parts for modular geminiviral...

...expression vectors for insertion of custom gRNA target sequences....

...Each isoform is uniquely barcoded with a 24 bp sequence, to simplify identification during screening. ...

... strand at each replication fork. Termination sequences present in the chromosomal DNA ensure that the...pSC101 itself. RepA binds to directly repeated sequences called iterons located near the ori and directly...ColIb-P9 plasmid, is complementary to part of the sequence forming this pseudoknot, so its binding prevents...replication may differ in the specialized proteins or sequences necessary for initiation or the mechanisms that...

...platform is compatible with enrichment, single-cell sequencing, and optical pooled screens in human cells. Addgene... improve the specificity of ssAAV, a targeting sequence of miR124T was inserted into the vector to silence...alone or in combination with miR124T targeting sequences, provides a simple yet valuable tool for microglia... REVeRT to functionally reconstituting coding sequences for a range of applications, including CRISPR ...

...inducible lambda-red gene, and an IPTG-inducible sgRNA sequence targeting the pMB1 replicon on pTargetF (Jiang... this system, you’d first introduce your sgRNA sequence into the pTargetF construct and then introduce... process begins with the addition of the sgRNA sequence in pEcgRNA. Next both plasmids are transformed...pTargetF, we replaced the original 20 bp specific sequences on pTargetF with the ccdB gene containing BsaI...

...longer stretches of sequence could be used. However, a practical limitation is sequencing depth and cost. ...variants with optimal function. High-throughput DNA sequencing is then used to measure the frequency of each...more codons you test in one library, the more sequencing reads you will need. See Fowler et al for more...

...phosphate backbone modifications The recognition sequence for CRISPR-Cas9 targeting is led by a ~20 bp gRNA...CRISPR RNA (crRNA). In addition to the 20 bp guide sequence, the crRNA also contains a ~20 bp repeat region...which is photocaged and unable to engage a target sequence. Upon a brief light exposure (several seconds)...

...in your organism or cell type? You can use DNA sequencing or other molecular cloning techniques to determine...resistance gene followed by the target site (the same sequence you’d like to mutate in the genome) and an out-of-frame.... A gRNA that targets your desired gene (whose sequence is also found in the reporter gene). A control...

...reconstituted in vivo to form a protein identical in sequence to the unmodified enzyme. Each half of the base... cell. Intein splitting removes any exogenous sequences and regenerates a native peptide bond at the split...markers, transformation acceptor vectors, and sequences for genomic integration by homology directed repair...

...working on lab operations software for next-gen sequencing (NGS), and high-throughput screening. While some...in the US to learn where the gaps are in their sequencing workflows, any problems they’re having with our...region. She is particularly interested in DNA sequencing tech, and the intersection of technology, software...

...: shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...viral vector for your gene of interest – an shRNA sequence specific to your gene’s mRNA or a sgRNA targeting...

... a protospacer flanking sequence, so you can target virtually any RNA sequence! In bacteria, Cas13d-mediated...The Cas13d proteins they identified have little sequence similarity to previously identified Cas13a-c orthologs...

...These vectors contain an Autonomously Replicating Sequence (ARS) derived from the yeast chromosome. As the...part of an ARS along with part of a centromere sequence (CEN). These vectors replicate as though they ...DNA (apparently independent of a known specific sequence) dictate the replication of these vectors. S. ...

...immunoprecipitation (ChIP) to allow purification of any genomic sequence specified by a particular gRNA. In the enChIP ...

...using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive genomic locus...

...These plasmids are created by your colleagues, sequence-verified, and widely cited in publications. All...