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  1. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

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    Blog Post
    ...forward. Here are a few out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring...what’s a scientist to do with no live audience in sight? You could do a talk virtually for your peer mentoring...university or use your current contacts to ask who they might suggest you speak with. See this post that’s all...

  2. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post
    ...their secondary structure, palindromic nature, and high GC content. This instability can cause partial loss...through ITRs due to their secondary structure and high GC content, but some companies now offer Sanger ...example, CAG is a strong promoter that drives very high, ubiquitous expression, while the human synapsin...

  3. Summer SciComm: Science Storytelling

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    Blog Post
    ...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...science storytelling. You can introduce your results right at the beginning of your communication, use conflict...storytelling options, and they all can be effective in the right circumstances.   However, in the midst of all these...

  5. Meet the New Addgene Board Members!

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    Blog Post
    ...Baradaran says.  Baradaran’s path to science began in high school. “I had a great chemistry teacher in 10th...medicines can have on human health. As a teenager in high school, I knew that this is what I wanted to do,...ClonTracer Barcoding Library, a library that “enables the high-resolution tracking of more than 1 million cancer...

  6. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    Blog Post
    ...and it is under copyright, you will need to obtain written approval from the copyright owner and will ...treatment conditions like drugs (Chemogenetics) or light (Optogenetics) to activate receptors? If you’re ...

  7. Fluorescent Biosensors for Measuring Autophagic Flux

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    Blog Post
    ...Keima accumulates in autolysosomes, this ratio is high. When untargeted, Keima can be used to measure bulk...autophagy-related microtubule-associated proteins 1A/1B light chain 3B, or LC3. LC3 is a commonly used marker ...oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources on Addgene.org...

  8. 10 Great Guest Posts We're Thankful For

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    Blog Post
    ...’d like to show our gratitude on the blog by highlighting some of the great posts written by Addgene depositors...to activate neurons and neuronal pathways using light and chemicals respectively. Didem Goz Aytruk from...

  10. Learning Technical Skills at a Lab Central Workshop

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    Blog Post
    ...but thought we could learn a little bit more about high throughput DNA prepping by trying out NEB’s new ...generating a wide range of insert sizes and also produced high yields of quality libraries from small amounts of...summarize my experience in my first blog post! I highly recommend doing the same if you ever go to a workshop...

  11. Visualizing Translation at the Single Molecule Level

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    Blog Post
    ...determine genome-wide translational frequency. Although highly useful, this technique requires extensive sample... nucleus to bind cognate untranslated mRNAs. The high specificity and resolution of this system are achieved...SunTag Catch up on Your GFP Background Choose the Brightest Fluorescent Protein Resources on Addgene.org ...

  13. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    Blog Post
    ...viral samples. Read on to learn how aqueous samples might be affected by dry ice, and specifically how dry... removing the caps, incubating the tubes in an upright position for 30 seconds, and then recapping the...to prevent any pH change, as we still observed a slight change in pH (from 7.4 to 6.8). It’s possible that...

  14. With Our New Viral Service, We're Taking CRISPR Further!

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    Blog Post
    ...as virus? “Like all things in science, getting a high titer Cas9 lentivirus prep takes a bit of time and...lentivirus, we are providing lentiviral preps containing highly requested gRNAs including those targeting EGFP,...library has retained gRNA representation, produce high titer lentivirus from the library, ensure gRNA representation...

  15. Four Factors that Differentiate the Stem Cell Field

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    Blog Post
    ...Director of Biology, Lianna Swanson, suggested that I might be able to attend the 10 Years of iPSCs Symposium...or tertiary, or non-existant). On the very first night of the iPSC symposium, a panel of thought leaders...Resources on the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach...

  16. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post
    ...this context, but also specifically study it. They might be researching enhancer elements that control the...research describe the production of plasmids that might be useful for your work. The links below direct ...techniques, the posts below may steer you in the right direction! CAPTURE-ing Chromatin Interactions: Using...

  17. Screening for Successful Genome Editing with Digital PCR

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    Blog Post
    ...of mutant target (as low as 20 pg or 0.02%) in a high background of wild type targets. Comparatively, ...M. A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells. PLoS...PubMed Central PMCID: PMC4735761. 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas...

  19. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...complicated? Well, while the techniques are straightforward, the systems – the cells growing and producing...And on top of that you need to worry about getting high enough expression of your protein of interest. If...by using Addgene’s Blue Flame award to identify highly requested GCE plasmids. But they’d also love scientist...

  20. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post
    ...variant that efficiently infects neurons, directs high-level transgene expression, and has retrograde functionality...genetic tool delivered by the AAV.   AAVrg requires high titers for efficient retrograde transport. For recombinase-dependent... at the injection site (Fischer, KB. 2019). The higher the titer, the more likely you are to see spurious...

  22. 7 Tips to Secure a STEAM Internship This Summer

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    Blog Post
    ...their former interns to fill entry-level positions right out of college. Prior to March 2020, internships... a nationwide database of  STEAM internships for high school, college, and graduate students. In order...interview, inspect your technology, background, and lighting for a smooth performance. Most importantly, make...

  24. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...narrow down search results by adding more terms.  Weighting We determined the relative importance of different...given resource, and designed the search engine to weight search results differently according to where a...matches of a name in the depositor field will rank higher than matches of the same name in the publication...

  25. Plasmids 101: Blue-white Screening

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    Blog Post
    ...). As shown on the representative plate to the right, colonies with an insert-containing plasmid have... plates at 4C for few hours after the initial overnight incubation increases pigment precipitation, enhances...colonies. Take care in making your plates: X-gal is light and temperature sensitive and needs to be added ...

  26. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...interested in the same things you are on a deep level. Right away you have something in common. Namely, the scientific... field will know about grants and projects that might help your work Tenure can depend on your national...some of my best studies were designed over late night poker games at Gordon Conferences). You don’t want...

  27. Human Germline Editing Using CRISPR

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    Blog Post
    ...zygotes at 18 hr post fertilization. They observed higher targeting efficiency than in iPSCs, but with the...scientists proceed with caution and with proper oversight from regulatory and ethics committees comprised... Check out Our New CRISPR Topic Page Read about High Fidelity CRISPR Variants Target Multiple Genes at...

  28. Antibodies 101: Conjugation

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    Blog Post
    ...antibodies that produce detectable signals like light or color. In this post, we’ll go over some of the...chemiluminescent reaction. Chemiluminescence, which is light produced by a chemical reaction, can be detected...Whatever the conjugate, the molecules must somehow be tightly linked to your antibody. This chemical coupling...

  29. Plasmids 101: The protein expression toolbox

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    Blog Post
    ...working with an endogenous locus. The most straightforward way to turn off expression permanently is to...- this section is for you! Many of the tools highlighted in this section can be used to turn expression...

  30. Summer SciComm Series: Cognitive Load

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    ... read an 11-page Cell paper - and sometimes, it might actually take me less time to read the book.  Intuitively...science communication, but the more you use, the higher cognitive load you are asking your audience to ...like the professor who once decided that to teach high school students PCR, they first had to understand...

  31. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    Blog Post
    ...have less complementarity to the gRNA, they have a higher propensity to reform double helices and decrease...measurements to determine which portions of Cas9 might be important for nuclease specificity. They found...PMC4176945. 4. Kleinstiver, Benjamin P., et al. "High-fidelity CRISPR–Cas9 nucleases with no detectable...

  32. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post
    ..., TdTomato was seen only in cells with GFP, at a high activation frequency of 56-93%. In the converse ...Cre-DOG lacks the toxicity that can be seen with high levels of transcription activation domains. Both...constructing split variants should be relatively straightforward, but it is also possible to create split versions...

  33. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post
    ...chronic myeloid leukemia. Bhang says the work highlighted the importance of developing more potent ABL1...clonal dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine 21.5 (2015...

  34. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...launched an online career coaching program that might make her coaching more affordable for you. There...transitioning a scientist from academia to industry, you might want to consult the Cheeky Scientist. Their motto...Successful Industry Professionals”. This larger shop might appeal to some although most scientist coaches can...

  35. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post
    ...undergraduate research questions. The idea is straightforward. Instructors first select a multi-gene pathway...O'Connor (left with Lab Director Doug Warner on the right) has taught courses in biochemistry, molecular cell...Addgene Videos to Learn about Basic Lab Techniques High School Students Explore Careers at Addgene Resources...

  36. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...understanding of cell biology. We hope that these high-quality, highly specific plasmids, as well as the instructions...flooding the cell with a plasmid containing regions of high homology to the gene with the cut, we trick the ...

  37. Progress Towards a PAM-Free CRISPR

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    Blog Post
    ...lucky" and find a great gRNA with a proximal PAM right where you need it, the odds of this occurring are...reliability. Notably, the SpRY variant didn’t display higher off-target effects than SpCas9, a pleasant surprise...stranded cleavage and base editing applications, with high editing capacity in both contexts for NRN PAMs and...

  38. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post
    ...endogenous retrovirus Rless glycoprotein (BaEVRless) had high rates of transduction for most cell types they tested...existing CRISPR delivery technologies, Nanoblades had higher rates for editing efficiency and lower rates of...zygotes, which results in albinism in mice. Of the eight mice born from Tyr Nanoblade treated zygotes, five...

  39. Mouse Modeling, Part 1: Genetically Engineered Mice

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    Blog Post
    .... In the first blog post in this series, I’ll highlight terminology you should be familiar with before...the use of long single-stranded DNA have led to a higher efficiency of homologous recombination for precise...

  40. Viral Vectors 101: Calcium Sensors

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    Blog Post
    ...ER lumen reaches the sub-millimolar range, much higher than in the cytosol. Thus, for the ER you would...subsequent change in fluorescence only occurs at high calcium concentrations. This will reduce noise and...simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased intracellular calcium...

  41. Viral Vectors 101: Chemogenetics

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    Blog Post
    ...recombinase systems. Picking Ligands Picking the right ligand is also important for building a chemogenetic...pharmacologically selective effector molecules (PSEMs). Higher potency means lower drug doses, which decreases... CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard H, Fishman GI, Conklin BR (1999) Conditional...

  42. CRISPR in the Clinic

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    ...host) as well as the introduction of the cancer-fighting component, CAR. Clinical trials are underway to...allele specifically. Early-stage trials showed a high success rate in clearing misfolded protein levels...Regarding effectiveness, if the editing frequency isn’t high enough to make a physiological difference, then ...

  43. Tips for Technical Support Calls

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    Blog Post
    ...company. This might seem like a no-brainer, but it happens more frequently than you might think. The Addgene... crucial information about a product is printed right on the tube itself! Is the answer to your question...

  44. Cloning Mammalian Cells with the Agarose Method

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    Blog Post
    ...colonies by (carefully) holding plates up to the light, where they appear as opaque dots (colonies should...the agarose onto the recipient plate at just the right temperature. If the agarose solution is too hot,...depending on the level of endogenous expression. We highly recommend the use of an electronic pipettor with...

  45. Truncated gRNAs for Regulating Gene Expression

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    Blog Post
    ... of interest thus represents a straightforward means of exerting tight control over Cas9 nuclease activity...different genetic loci within a single cell. One might imagine transfecting cells with both the nuclease-positive...

  46. Polymerase Chain Reaction Overview and Applications

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    Blog Post
    ...primers.  Extension- The temperature is then raised slightly to Taq polymerase’s ideal temperature (70-75oC...which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion droplet technology...those stated above. Multiplex PCR is also used in high throughput SNP genotyping, gene deletion analysis...

  47. 3D Printing Meets CRISPR Cas9

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    Blog Post
    ...something silly like “See there, arginine 53 really is right there, at the end of helix 3…just like we said it...are useful. While a physical model of a protein might represent one aspect of the protein very well, it...while and you will wake up in the middle of the night dreaming about the conformational gymnastics that...

  48. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post
    ... Sometimes people don’t know how to start. They might want to use our promoters and terminators, but then...coding sequence to the MoClo system. It’s quite straightforward really and we’ll be producing an instructional...chances of doing something amazing will be equally high as for those who go to work in cancer biology. ...

  49. Tips for Using BLAST to Verify Plasmids

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    Blog Post
    ...to make the most of your time and sequence! You might also enjoy seeing how our quality control process...alignment results. The default setting is “Optimize for Highly similar sequences (megablast)”, which is very fast...nucleotide sequences, proteins often have repeated or highly homologous regions, which by default are ignored...

  50. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post
    ...a collection of promises to not assert property rights for particular engineered genetic sequences; but...metabolite in a cell. The only way to do that at scale right now is to grow up a bunch of cells, sacrifice them...chemicals, to make more medicine. The parts we have right now are well adapted to work in prokaryotic bacteria...
Showing: 651 - 700 of 986 results