...focused on translational research is encouraging. Patients are key to the research, but they are also the...observations regarding what makes this group of researchers special: 1. Stem cell scientists care deeply ...Listen to Our Podcast Interview with Stem Cell Researcher Darrell Kotton 2. Stem cell scientists care about... on spinal cord injuries; Jessica Young shared research on stem cells and Alzheimer's Disease; and Kevin...the reason this research is happening. Stem cells scientists have certainly not lost site of that. 3. Stem...acquisition is not a rate-limiting step in stem cell research. People told me they couldn't have done their ... to helping the stem cell community with their research, I think I can speak for Addgene when I say that...

...Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers: Cynthia Arokiaraj...work with Addgenie writers to help share their research, tools, and knowledge. Guest authors The Codon... Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew...NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase...Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv YanevCellosaurus: A Cell...ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers: Atharva Sahasrabudhe; Polina Aniveeka   A...blog this year! Your contributions have helped researchers around the globe find and share knowledge, resources...

...statement: Accelerate research and discovery by improving access to useful research materials and information...post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were...examples of technologies that can accelerate your research. The creation of NanoLuc® Luciferase from a rather... or above 600 nm wavelengths should be used. Researchers often use bioluminescence to overcome these issues...brightness of NLuc has caught the attention of researchers wanting to perform whole-animal imaging, however...leads to spurious activation of the FRET sensor. Researchers have sought to alleviate many of these challenges...also activate the optogenetic accuators. The researchers took a FRET reporter and adapted it into a BRET...

...Novartis researcher, Carrie Bhang. The ClonTracer Library, deposited by Carrie Bhang, a research investigator...was actually really research oriented [and] somewhat similar to an academic research environment” with ...Oncology, is an exciting new tool that allows researchers to individually label millions of mammalian cells...because she wanted first-hand experience in industry research. When she came to Novartis, she was happily surprised...easily be missed. The ClonTracer library allows a researcher to label individual members of a starting population...she wanted to make it as easy as possible for researchers to use this tool in their own work. She says,...resource to the Addgene community! Carrie Bhang is a research investigator in In Vivo Pharmacology at Novartis...

...various light-controlled CRISPR tools available to researchers - some readily found at Addgene. Shining light...position to activate gene transcription. The researchers tested a variety of combinations to optimize ...pattern during blue light exposure (470nm), the researchers showed that expression of the human ASCL1 gene...cells to induce expression of human IL1RN, the researchers saw an 11-fold increase in mRNA levels after ...cycled on-off-on. Using a slit photomask, the researchers also demonstrated the ability to spatially control...-mediated CRISPR labeling to study chromatin architecture (Hoffman et al., 2020). Fluorescent dCas9 proteins...can potentially alter the genome and chromatin architecture, it is useful to regulate the time in which ...

...Addgene to accelerate their research. Read on to learn how these researchers plan to use plasmids from ...Genomics Institute to provide plasmid grants to researchers working on rare diseases through the BeHEARD ...from Addgene to advance their research on diseases of glycosylation, arginine mutagenesis, and neural development...She leads the Glycoimmunology group based at the Research Unit on Applied Molecular Biosciences (UCIBIO)... (APCDG) and an international platform for CDG research, named the CDG&Allies – Professionals and Patient... Colorado, USA. The primary focus of his lab’s research is the mechanisms by which proteins assemble into... a professor at the University of Buffalo. His research focuses on understanding the structural determinants...

...increasingly popular in fundamental and applied research since their first use in the early 90’s to genetically...inefficient at infecting non-dividing cells and so researchers set out to create a better, safer system. Unlike...than the first one and can be used routinely in a research laboratory. The risk of generating RCL is low ...technique, there’s always a small chance that a researcher could infect him or herself with the oncogenic... generation lentiviral vectors can be used for research and for clinical purposes, however improving the...safety of these vectors is still an active area of research due to the possibility that mutation or recombination...lentiviruses with human tropism can still infect the researcher and insert into her/his genome - this is a biosafety...

...10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage...plasmids every quarter. The “hot plasmids” from last March were quite popular and represent the diversity of...academic job interview including defining your research vision, organizing your vision into a chalk talk...many model organisms people are using for their research. In this blog post, we’ve covered five model organisms...yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics... technique in molecular biology and scientific research. Get an overview of these methods in this blog...fantastic year and happy reading. Best wishes on your research in 2020! Additional resources on the Addgene ...

...Addgene.org Find plasmids for your research Find cancer research plasmids and resources ...represents a considerable barrier to furthering research and developing effective treatments for the disease...work, and how these plasmids can help cancer researchers studying an assortment of genes. Using patient...Anderson Cancer Center patient databases, this research team identified 1,049 perturbations in several...functional annotation of thousands of mutations The researchers used the collection to screen individual perturbations... platform is an incredible boost to the cancer research community, but what else are these plasmids useful...Some of the most heavily-studied genes in cancer research, like PIK3CA (Plasmid 116771: pHAGE-PIK3CA) and...

...  Easily search for plasmids (and more!) The main goal for the homepage was to improve search and browse...redesign, the new search bar is now front and center, with a clear call to action - Search! Along with this...It became clear to us that we needed to improve search and navigation, while also updating brand messaging...Community, sharing, and fun Along with improving search and navigation, we also wanted to emphasize the...the Addgene blog Learn more about Addgene's new search tool Read more about other features of Addgene'...website Resources on Addgene.org Try out the new search on Addgene's homepage ...

...in full-length publications. As early career researchers, we wondered how we could make the insights gained...around reading through entire papers, perhaps you search google images for specific experimental results...These data are valuable but remain hidden from research papers as they are not included in traditional...study with multiple patients was published, Zika researcher Christa Osuna collated Zika case studies, making... the NIH and Wellcome Trust now accept interim research products (pre-registration, preprints, data, software...micropublications) in grant proposals. ReFigures are one such research product which can convince reviewers of your expertise...recommend sharing ReFigures on your Linkedin and ResearchGate profile. To see how to create ReFigures in a...

...mission: Accelerate research and discovery by improving access to useful research materials and information...which we promote our mission and work to enable researchers around the world. We take this responsibility... by generating revenue through distribution of research materials. Our small shipping and handling fees...materials shared through Addgene are being used for research purposes and not for a profit motive. We build... we will always look for new ways to serve the research community. Questions? Feel free to reach out to...science and Addgene in our mission to accelerate research with easier sharing and collaboration. Joanne ...Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview with Addgene Board Member...

...your favorite search term, i.e. “biology + masters.” For lab-based roles, look for “research associate,”...have masters by using the search term “master’s degree.” Use the LinkedIn search filters to limit results...postings  Look at master’s-level job postings on a job search website like Indeed. Do the job descriptions sound...manuscript and grant preparation, and lead your own research project.  3. Do you prefer being a generalist?...variety of projects and in different fields of research is one of the best parts of my career with a master...master’s. While it's possible to change fields of research with a PhD, a master’s is less specialized so ...

...mechanism of incorporation is not well understood, researchers have been using AAV vectors for gene editing ...iPSCs from ~0.2% to ~1.5%. In more recent work, researchers have combined AAVs with CRISPR systems. Ohmori...an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods to deliver CRISPR components...delivery method frees up space in the AAV allowing a researcher to fill it with the repair template. Bok and ...hematopoietic stem cells respectively. Future researchers could apply this technique to efficiently deliver... continue to use and develop AAVs for your own research and encourage to deposit any new AAV tools you...Cancer Center, Georgetown School of Medicine. His research currently focuses on determining the mechanisms...

...Plasmids from:  March (Q1) | June (Q2) | September 2016 (Q3) | December 2016 (Q3) MARCH 2016Enhancer activity... much literature searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore... states. Alexander Stark and colleagues at the Research Institute of Molecular Pathology have developed... library     CRISPR pooled libraries enable researchers to screen the entire genome for genes that regulate...newly available PRESTO-TANGO kit which allows researchers to interrogate the druggable human GPCR-ome using...utility of this platform for drug discovery. Researchers can use plasmids from the PRESTO-TANGO kit directly...-disciplinary Integration of Design Automation Research lab Moduclar Cloning (CIDAR MoClo) Parts Kit. ...

...database is to browse or search information in more powerful ways, and the advanced search page simplifies the...contributed by guest blogger Talley Lambert, a Research Associate at Harvard Medical School. The need ... most indispensable tools in modern biological research.  Any microscopist will tell you that selection...has been very useful over the years, and when researchers come to us for advice on choosing a protein, ...particular FP; this can be done directly using the search bar on the homepage (and at the top of all pages...the process of building advanced multi-parametric search queries.  Looking for a bright, monomeric FP published...guest blogger Talley Lambert! Talley Lambert is a Research Associate at Harvard Medical School. He manages...

... results helps other researchers using these plasmids and will give your research more visibility.    ...By Will Arnold and Shreya Vedantam Accelerating research by improving the availability of materials is ...to help academic and industrial labs accelerate research and to help find a faster solution to the COVID...coli and S. cerevisiae expression vectors in late March. The first set of 383 constructs went online just...samples, the rest will be put into a status we call “Archived.” This status allows us to store plasmids and ... is indeed global. Helping scientists speed up research is what we’re here for and we’re glad to be able...Ginkgo Bioworks  Find COVID-19 plasmids for your research Browse the Ginkgo Bioworks COVID-19 Plasmid Collection...

...A 2017 survey found that many researchers feel they lack formal training in a variety of transferable...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... into play while puzzling through your broader research questions and designing future experiments. Being...While I was in graduate school and beginning to research non-academic careers, I always felt that descriptions...when you’re faced with yet another hurdle in your research journey.  What are ‘problem solving skills’? As... about the world. You might do some background research to better understand it and come up with several... of your skill set later on. If you are new to research and the lab, you may not be able to carry out ...

...represents a unique funding model in nuclear receptor research, and has generated a set of comprehensive datasets... questions in nuclear receptor and coregulator research. Our Q-PCR datasets, for example, document the... today.  The sheer abundance of these critical research tools is a double-edged sword, however, and has...burden associated with identifying plasmids in the research literature and acquiring them in a timely manner...to the problem of sharing plasmids, providing researchers with a one-stop shop for plasmids and vectors...vectors, and making them available to all researchers for a reasonable fee. We at NURSA recognize the importance...importance of this service to the NR research community and, accordingly, the new version of the NURSA website...

...off-target cleavage events. Unbiased techniques search the whole genome for off-target sites irrespective... in concert, these approaches can provide the researcher with a reasonable level of certainly that the...sgRNA. This is a good starting point for most researchers as it provides a list of putative off-target ...can later sequence for mutations. One method a researcher can use to test predicted off-target sites following...to perform these validations as translational researchers begin to use CRISPR as a therapy. Low frequency... effects may generate irreproducible data in a research setting, but these events could have disastrous...off-target prediction algorithms, their genome-wide search criteria are not exhaustive. Mismatch tolerance...